Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle
The beef cattle rumen is a heterogenous microbial ecosystem that is necessary for the host to digest food and support growth. The importance of the rumen microbiota (RM) is also widely recognized for its critical roles in metabolism and immunity. The level of health is indicated by a dynamic RM dist...
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00251/full |
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| author | Fengmei Chen Guangmin Cheng Yulin Xu Yulin Xu Yulin Xu Yulin Xu Yunzhou Wang Qingxiang Xia Shilin Hu |
| author_facet | Fengmei Chen Guangmin Cheng Yulin Xu Yulin Xu Yulin Xu Yulin Xu Yunzhou Wang Qingxiang Xia Shilin Hu |
| author_sort | Fengmei Chen |
| collection | DOAJ |
| description | The beef cattle rumen is a heterogenous microbial ecosystem that is necessary for the host to digest food and support growth. The importance of the rumen microbiota (RM) is also widely recognized for its critical roles in metabolism and immunity. The level of health is indicated by a dynamic RM distribution. We performed high-throughput sequencing of the bacterial 16S rRNA gene to compare microbial populations between rumens in beef cattle with or without doxycycline treatment to assess dynamic microbiotic shifts following antibiotic administration. The results of the operational taxonomic unit analysis and alpha and beta diversity calculations showed that doxycycline-treated beef cattle had lower species richness and bacterial diversity than those without doxycycline. Bacteroidetes was the predominant phylum in rumen samples without doxycycline, while Proteobacteria was the governing phylum in the presence of doxycycline. On the family level, the top three predominant populations in group qlqlwy (not treated with doxycycline) were Prevotellaceae, Lachnospiraceae, and Ruminococcaceae, compared to Xanthomonadaceae, Prevotellaceae, and Rikenellaceae in group qlhlwy (treated with doxycycline). At the genus level, the top predominant population in group qlqlwy was unidentified_Prevotellaceae. However, in group qlhlwy, the top predominant population was Stenotrophomonas. The results revealed significant RM differences in beef cattle with or without doxycycline. Oral doxycycline may induce RM composition differences, and bacterial richness may also influence corresponding changes that could guide antibiotic use in adult ruminants. This study is the first to assess microbiota distribution in beef cattle rumen after doxycycline administration. |
| first_indexed | 2024-12-11T04:58:17Z |
| format | Article |
| id | doaj.art-75c5597e6e584b6b9abe6fc47e30fc1c |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-11T04:58:17Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-75c5597e6e584b6b9abe6fc47e30fc1c2022-12-22T01:20:13ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-06-01710.3389/fvets.2020.00251505290Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef CattleFengmei Chen0Guangmin Cheng1Yulin Xu2Yulin Xu3Yulin Xu4Yulin Xu5Yunzhou Wang6Qingxiang Xia7Shilin Hu8Shandong Research Center for Technology of Reduction of Antibiotics Administered to Animal and Poultry, Shandong Vocational Animal Science and Veterinary College, Weifang, ChinaShandong Research Center for Technology of Reduction of Antibiotics Administered to Animal and Poultry, Shandong Vocational Animal Science and Veterinary College, Weifang, ChinaComparative Medicine Research Institute, Yangzhou University, Yangzhou, ChinaCollege Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, ChinaShandong Research Center for Technology of Reduction of Antibiotics Administered to Animal and Poultry, Shandong Vocational Animal Science and Veterinary College, Weifang, ChinaShandong Research Center for Technology of Reduction of Antibiotics Administered to Animal and Poultry, Shandong Vocational Animal Science and Veterinary College, Weifang, ChinaShandong Research Center for Technology of Reduction of Antibiotics Administered to Animal and Poultry, Shandong Vocational Animal Science and Veterinary College, Weifang, ChinaThe beef cattle rumen is a heterogenous microbial ecosystem that is necessary for the host to digest food and support growth. The importance of the rumen microbiota (RM) is also widely recognized for its critical roles in metabolism and immunity. The level of health is indicated by a dynamic RM distribution. We performed high-throughput sequencing of the bacterial 16S rRNA gene to compare microbial populations between rumens in beef cattle with or without doxycycline treatment to assess dynamic microbiotic shifts following antibiotic administration. The results of the operational taxonomic unit analysis and alpha and beta diversity calculations showed that doxycycline-treated beef cattle had lower species richness and bacterial diversity than those without doxycycline. Bacteroidetes was the predominant phylum in rumen samples without doxycycline, while Proteobacteria was the governing phylum in the presence of doxycycline. On the family level, the top three predominant populations in group qlqlwy (not treated with doxycycline) were Prevotellaceae, Lachnospiraceae, and Ruminococcaceae, compared to Xanthomonadaceae, Prevotellaceae, and Rikenellaceae in group qlhlwy (treated with doxycycline). At the genus level, the top predominant population in group qlqlwy was unidentified_Prevotellaceae. However, in group qlhlwy, the top predominant population was Stenotrophomonas. The results revealed significant RM differences in beef cattle with or without doxycycline. Oral doxycycline may induce RM composition differences, and bacterial richness may also influence corresponding changes that could guide antibiotic use in adult ruminants. This study is the first to assess microbiota distribution in beef cattle rumen after doxycycline administration.https://www.frontiersin.org/article/10.3389/fvets.2020.00251/fullbeef cattlerumen microbiotadoxycyclineMiSeq sequencingdysbacteriosisoral antibiotics |
| spellingShingle | Fengmei Chen Guangmin Cheng Yulin Xu Yulin Xu Yulin Xu Yulin Xu Yunzhou Wang Qingxiang Xia Shilin Hu Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle Frontiers in Veterinary Science beef cattle rumen microbiota doxycycline MiSeq sequencing dysbacteriosis oral antibiotics |
| title | Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle |
| title_full | Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle |
| title_fullStr | Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle |
| title_full_unstemmed | Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle |
| title_short | Rumen Microbiota Distribution Analyzed by High-Throughput Sequencing After Oral Doxycycline Administration in Beef Cattle |
| title_sort | rumen microbiota distribution analyzed by high throughput sequencing after oral doxycycline administration in beef cattle |
| topic | beef cattle rumen microbiota doxycycline MiSeq sequencing dysbacteriosis oral antibiotics |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.00251/full |
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