Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran
Background & Aims: Blastocystis sp. is a common parasite of humans with a vast variety of nonhuman hosts and global distribution. Based on molecular methods, distinct subtypes are isolated from different hosts. In this study, we detected human-isolated Blastocystis subtypes in the northwestern o...
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| Format: | Article |
| Language: | English |
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Urmia University of Medical Sciences
2022-11-01
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| Series: | Health Science Monitor |
| Subjects: | |
| Online Access: | http://hsm.umsu.ac.ir/article-1-73-en.pdf |
| _version_ | 1797377422660730880 |
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| author | Mehrdad Rostami Arezoo Bozorgomid Hadi Mirahmadi Saber Raeghi |
| author_facet | Mehrdad Rostami Arezoo Bozorgomid Hadi Mirahmadi Saber Raeghi |
| author_sort | Mehrdad Rostami |
| collection | DOAJ |
| description | Background & Aims: Blastocystis sp. is a common parasite of humans with a vast variety of nonhuman hosts and global distribution. Based on molecular methods, distinct subtypes are isolated from different hosts. In this study, we detected human-isolated Blastocystis subtypes in the northwestern of Iran.
Materials & Methods: A total of 600 stool samples referred to health centers in Urmia and Maragheh cities in the northwest of Iran was randomly collected and examined using direct wet mount, formalin-ether concentration, a modified version of the Ziehl–Neelsen staining technique for the detection of parasitological items and using barcoding method (18S rRNA gene) for the molecular survey.
Results: From 600 samples studied, 496 (82.7%) and 104 (17.3%) were male and female, respectively. Three subtypes, including ST1, ST2 and ST3, were identified from collected samples, and the frequent subtypes were ST3, ST1, and ST2, respectively.
Conclusion: While genetic similarity of Blastocystis isolated from human and animal did not show in this region, the zoonotic cycle of this parasite, probably by fecal contamination, exists in the study area. By identifying Blastocystis subtypes, it is possible to prevent the transmission and related complications. |
| first_indexed | 2024-03-08T19:52:18Z |
| format | Article |
| id | doaj.art-75c775da228e472e9774975029a6db88 |
| institution | Directory Open Access Journal |
| issn | 2980-8723 |
| language | English |
| last_indexed | 2024-03-08T19:52:18Z |
| publishDate | 2022-11-01 |
| publisher | Urmia University of Medical Sciences |
| record_format | Article |
| series | Health Science Monitor |
| spelling | doaj.art-75c775da228e472e9774975029a6db882023-12-24T07:31:42ZengUrmia University of Medical SciencesHealth Science Monitor2980-87232022-11-0112109115Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of IranMehrdad Rostami0Arezoo Bozorgomid1Hadi Mirahmadi2Saber Raeghi3 Department of Hematology and Blood Bank, Mashhad University of Medical Sciences, Mashhad, Iran Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran AND Dept. of Parasitology, Mycology and Entomology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran Background & Aims: Blastocystis sp. is a common parasite of humans with a vast variety of nonhuman hosts and global distribution. Based on molecular methods, distinct subtypes are isolated from different hosts. In this study, we detected human-isolated Blastocystis subtypes in the northwestern of Iran. Materials & Methods: A total of 600 stool samples referred to health centers in Urmia and Maragheh cities in the northwest of Iran was randomly collected and examined using direct wet mount, formalin-ether concentration, a modified version of the Ziehl–Neelsen staining technique for the detection of parasitological items and using barcoding method (18S rRNA gene) for the molecular survey. Results: From 600 samples studied, 496 (82.7%) and 104 (17.3%) were male and female, respectively. Three subtypes, including ST1, ST2 and ST3, were identified from collected samples, and the frequent subtypes were ST3, ST1, and ST2, respectively. Conclusion: While genetic similarity of Blastocystis isolated from human and animal did not show in this region, the zoonotic cycle of this parasite, probably by fecal contamination, exists in the study area. By identifying Blastocystis subtypes, it is possible to prevent the transmission and related complications.http://hsm.umsu.ac.ir/article-1-73-en.pdfblastocystis sp.18s rrnanorthwestern of iran |
| spellingShingle | Mehrdad Rostami Arezoo Bozorgomid Hadi Mirahmadi Saber Raeghi Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran Health Science Monitor blastocystis sp. 18s rrna northwestern of iran |
| title | Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran |
| title_full | Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran |
| title_fullStr | Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran |
| title_full_unstemmed | Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran |
| title_short | Molecular phylogenetic identification of Blastocystis sp. isolated from humans in the Northwest of Iran |
| title_sort | molecular phylogenetic identification of blastocystis sp isolated from humans in the northwest of iran |
| topic | blastocystis sp. 18s rrna northwestern of iran |
| url | http://hsm.umsu.ac.ir/article-1-73-en.pdf |
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