Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)

We performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long,...

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Main Authors: Kamil Ciborowski, Bartosz Skierkowski, Karolina Żukowska, Katarzyna Krawczyk, Jakub Sawicki
Format: Article
Language:English
Published: Taylor & Francis Group 2022-08-01
Series:Mitochondrial DNA. Part B. Resources
Subjects:
Online Access:http://dx.doi.org/10.1080/23802359.2022.2107458
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author Kamil Ciborowski
Bartosz Skierkowski
Karolina Żukowska
Katarzyna Krawczyk
Jakub Sawicki
author_facet Kamil Ciborowski
Bartosz Skierkowski
Karolina Żukowska
Katarzyna Krawczyk
Jakub Sawicki
author_sort Kamil Ciborowski
collection DOAJ
description We performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long, the large single copy (LSC) region is 80,606 bp long, the small single copy (SSC) region is 19,401 bp long. The nanopore sequencing allowed for the distinction of inverted repeats A and B (IRA, IRB) which were not identical. Guanine cytosine (GC) ratio is 33.7% for the complete chloroplast genome, for LSC and SSC are 31.7% and 30.6%. The phylogeny reconstruction shows that Scapania undulata is the sister to S. ciliata and S. griffithii.
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spelling doaj.art-75cb9937b9484d28bdafbf62a04b03682023-11-03T14:44:04ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592022-08-01781424142610.1080/23802359.2022.21074582107458Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)Kamil Ciborowski0Bartosz Skierkowski1Karolina Żukowska2Katarzyna Krawczyk3Jakub Sawicki4Department of Botany and Nature Protection, University of Warmia and Mazury in OlsztynDepartment of Botany and Nature Protection, University of Warmia and Mazury in OlsztynDepartment of Botany and Nature Protection, University of Warmia and Mazury in OlsztynDepartment of Botany and Nature Protection, University of Warmia and Mazury in OlsztynDepartment of Botany and Nature Protection, University of Warmia and Mazury in OlsztynWe performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long, the large single copy (LSC) region is 80,606 bp long, the small single copy (SSC) region is 19,401 bp long. The nanopore sequencing allowed for the distinction of inverted repeats A and B (IRA, IRB) which were not identical. Guanine cytosine (GC) ratio is 33.7% for the complete chloroplast genome, for LSC and SSC are 31.7% and 30.6%. The phylogeny reconstruction shows that Scapania undulata is the sister to S. ciliata and S. griffithii.http://dx.doi.org/10.1080/23802359.2022.2107458liverwortchloroplast genomescapaniananopore
spellingShingle Kamil Ciborowski
Bartosz Skierkowski
Karolina Żukowska
Katarzyna Krawczyk
Jakub Sawicki
Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
Mitochondrial DNA. Part B. Resources
liverwort
chloroplast genome
scapania
nanopore
title Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_full Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_fullStr Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_full_unstemmed Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_short Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_sort nanopore sequencing of chloroplast genome of scapania undulata l dumort 1835 scapaniaceae jungermanniales
topic liverwort
chloroplast genome
scapania
nanopore
url http://dx.doi.org/10.1080/23802359.2022.2107458
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