Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia
Abstract Grona styracifolia is a photophilous legume that contains abundant flavonoids with multiple pharmacological activities, which is used to cure urethral and biliary calculus in China for thousands of years. The authentication of the rate-limiting enzymes involved in the flavonoids biosynthesi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-06-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04290-z |
| _version_ | 1797811548215836672 |
|---|---|
| author | Chunzhu Xie Ting Zhan Jinqin Huang Jun Lan Lingling Shen Hongbin Wang Xiasheng Zheng |
| author_facet | Chunzhu Xie Ting Zhan Jinqin Huang Jun Lan Lingling Shen Hongbin Wang Xiasheng Zheng |
| author_sort | Chunzhu Xie |
| collection | DOAJ |
| description | Abstract Grona styracifolia is a photophilous legume that contains abundant flavonoids with multiple pharmacological activities, which is used to cure urethral and biliary calculus in China for thousands of years. The authentication of the rate-limiting enzymes involved in the flavonoids biosynthesis pathway enabled a better understanding of the molecular aspect of quality formation and modulation of this medicinal herb. In this study, the chemical distribution characteristics and content of flavonoids in different tissues of Grona styracifolia were analyzed using ultraperormance liquid chromatography coupled with Q-TOF mass spectrometry and showed that active flavonoids were primarily synthesized and stored in the leaves. Subsequently, RNA sequencing (RNA-seq)-based transcriptome profiling of the different tissues revealed that the flavonoids biosynthesis in the leaves was the most active. Meanwhile, 27 full-length transcripts inferred encoding vital enzymes involved in the flavonoids biosynthesis were preliminarily excavated. Finally, four CHSs, four CHIs, and one FNSII were successfully characterized by heterologous expression, which involved in three rate-limiting steps of the flavonoid biosynthetic pathway. In conclusion, these results laid a foundation for further investigation of the molecular mechanism of the biosynthesis and modulation of active flavonoids in Grona styracifolia. |
| first_indexed | 2024-03-13T07:25:23Z |
| format | Article |
| id | doaj.art-75ce1f17a27744bbbda82f76d1828384 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-13T07:25:23Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-75ce1f17a27744bbbda82f76d18283842023-06-04T11:24:54ZengBMCBMC Plant Biology1471-22292023-06-0123111310.1186/s12870-023-04290-zFunctional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifoliaChunzhu Xie0Ting Zhan1Jinqin Huang2Jun Lan3Lingling Shen4Hongbin Wang5Xiasheng Zheng6Institute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineInstitute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineInstitute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineInstitute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineGuangzhou Analytical Applications Center, Shimadzu (China) Co., LTDInstitute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineInstitute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineAbstract Grona styracifolia is a photophilous legume that contains abundant flavonoids with multiple pharmacological activities, which is used to cure urethral and biliary calculus in China for thousands of years. The authentication of the rate-limiting enzymes involved in the flavonoids biosynthesis pathway enabled a better understanding of the molecular aspect of quality formation and modulation of this medicinal herb. In this study, the chemical distribution characteristics and content of flavonoids in different tissues of Grona styracifolia were analyzed using ultraperormance liquid chromatography coupled with Q-TOF mass spectrometry and showed that active flavonoids were primarily synthesized and stored in the leaves. Subsequently, RNA sequencing (RNA-seq)-based transcriptome profiling of the different tissues revealed that the flavonoids biosynthesis in the leaves was the most active. Meanwhile, 27 full-length transcripts inferred encoding vital enzymes involved in the flavonoids biosynthesis were preliminarily excavated. Finally, four CHSs, four CHIs, and one FNSII were successfully characterized by heterologous expression, which involved in three rate-limiting steps of the flavonoid biosynthetic pathway. In conclusion, these results laid a foundation for further investigation of the molecular mechanism of the biosynthesis and modulation of active flavonoids in Grona styracifolia.https://doi.org/10.1186/s12870-023-04290-zGrona styracifoliaActive flavonoidsBiosynthetic pathway |
| spellingShingle | Chunzhu Xie Ting Zhan Jinqin Huang Jun Lan Lingling Shen Hongbin Wang Xiasheng Zheng Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia BMC Plant Biology Grona styracifolia Active flavonoids Biosynthetic pathway |
| title | Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia |
| title_full | Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia |
| title_fullStr | Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia |
| title_full_unstemmed | Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia |
| title_short | Functional characterization of nine critical genes encoding rate-limiting enzymes in the flavonoid biosynthesis of the medicinal herb Grona styracifolia |
| title_sort | functional characterization of nine critical genes encoding rate limiting enzymes in the flavonoid biosynthesis of the medicinal herb grona styracifolia |
| topic | Grona styracifolia Active flavonoids Biosynthetic pathway |
| url | https://doi.org/10.1186/s12870-023-04290-z |
| work_keys_str_mv | AT chunzhuxie functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia AT tingzhan functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia AT jinqinhuang functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia AT junlan functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia AT linglingshen functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia AT hongbinwang functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia AT xiashengzheng functionalcharacterizationofninecriticalgenesencodingratelimitingenzymesintheflavonoidbiosynthesisofthemedicinalherbgronastyracifolia |