Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia
(1) Background: Amplicon-based 16S rRNA profiling is widely used to study whole communities of prokaryotes in many niches. Here, we comparatively examined the microbial composition of three tick species, <i>Ixodes ricinus</i>, <i>Ixodes persulcatus</i> and <i>Dermacento...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/8/1970 |
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| author | Agne Namina Alisa Kazarina Marija Lazovska Sarmite Akopjana Viktorija Ulanova Agnija Kivrane Lauma Freimane Darja Sadovska Janis Kimsis Antra Bormane Valentina Capligina Renate Ranka |
| author_facet | Agne Namina Alisa Kazarina Marija Lazovska Sarmite Akopjana Viktorija Ulanova Agnija Kivrane Lauma Freimane Darja Sadovska Janis Kimsis Antra Bormane Valentina Capligina Renate Ranka |
| author_sort | Agne Namina |
| collection | DOAJ |
| description | (1) Background: Amplicon-based 16S rRNA profiling is widely used to study whole communities of prokaryotes in many niches. Here, we comparatively examined the microbial composition of three tick species, <i>Ixodes ricinus</i>, <i>Ixodes persulcatus</i> and <i>Dermacentor reticulatus</i>, which were field-collected in Latvia. (2) Methods: Tick DNA samples were used for microbiome analysis targeting bacterial 16S rDNA using next-generation sequencing (NGS). (3) Results: The results showed significant differences in microbial species diversity and composition by tick species and life stage. A close similarity between microbiomes of <i>I. ricinus</i> and <i>I. persulcatus</i> ticks was observed, while the <i>D. reticulatus</i> microbiome composition appeared to be more distinct. Significant differences in alpha and beta microbial diversity were observed between <i>Ixodes</i> tick life stages and sexes, with lower taxa richness indexes obtained for female ticks. The <i>Francisella</i> genus was closely associated with <i>D. reticulatus</i> ticks, while endosymbionts <i>Candidatus Midichlorii</i> and <i>Candidatus Lariskella</i> were associated with <i>I. ricinus</i> and <i>I. persulcatus</i> females, respectively. In <i>I. ricinus</i> females, the endosymbiont load negatively correlated with the presence of the <i>Rickettsia</i> genus. (4) Conclusions: The results of this study revealed important associations between ticks and their microbial community and highlighted the microbiome features of three tick species in Latvia. |
| first_indexed | 2024-03-10T23:43:40Z |
| format | Article |
| id | doaj.art-75cee1474e12484e8e5856582a5f6060 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T23:43:40Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-75cee1474e12484e8e5856582a5f60602023-11-19T02:16:56ZengMDPI AGMicroorganisms2076-26072023-07-01118197010.3390/microorganisms11081970Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in LatviaAgne Namina0Alisa Kazarina1Marija Lazovska2Sarmite Akopjana3Viktorija Ulanova4Agnija Kivrane5Lauma Freimane6Darja Sadovska7Janis Kimsis8Antra Bormane9Valentina Capligina10Renate Ranka11Latvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, LV-1067 Riga, Latvia(1) Background: Amplicon-based 16S rRNA profiling is widely used to study whole communities of prokaryotes in many niches. Here, we comparatively examined the microbial composition of three tick species, <i>Ixodes ricinus</i>, <i>Ixodes persulcatus</i> and <i>Dermacentor reticulatus</i>, which were field-collected in Latvia. (2) Methods: Tick DNA samples were used for microbiome analysis targeting bacterial 16S rDNA using next-generation sequencing (NGS). (3) Results: The results showed significant differences in microbial species diversity and composition by tick species and life stage. A close similarity between microbiomes of <i>I. ricinus</i> and <i>I. persulcatus</i> ticks was observed, while the <i>D. reticulatus</i> microbiome composition appeared to be more distinct. Significant differences in alpha and beta microbial diversity were observed between <i>Ixodes</i> tick life stages and sexes, with lower taxa richness indexes obtained for female ticks. The <i>Francisella</i> genus was closely associated with <i>D. reticulatus</i> ticks, while endosymbionts <i>Candidatus Midichlorii</i> and <i>Candidatus Lariskella</i> were associated with <i>I. ricinus</i> and <i>I. persulcatus</i> females, respectively. In <i>I. ricinus</i> females, the endosymbiont load negatively correlated with the presence of the <i>Rickettsia</i> genus. (4) Conclusions: The results of this study revealed important associations between ticks and their microbial community and highlighted the microbiome features of three tick species in Latvia.https://www.mdpi.com/2076-2607/11/8/1970tick microbiome<i>Ixodes ricinus</i><i>Ixodes persulcatus</i><i>Dermacentor reticulatus</i>Latvia |
| spellingShingle | Agne Namina Alisa Kazarina Marija Lazovska Sarmite Akopjana Viktorija Ulanova Agnija Kivrane Lauma Freimane Darja Sadovska Janis Kimsis Antra Bormane Valentina Capligina Renate Ranka Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia Microorganisms tick microbiome <i>Ixodes ricinus</i> <i>Ixodes persulcatus</i> <i>Dermacentor reticulatus</i> Latvia |
| title | Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia |
| title_full | Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia |
| title_fullStr | Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia |
| title_full_unstemmed | Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia |
| title_short | Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia |
| title_sort | comparative microbiome analysis of three epidemiologically important tick species in latvia |
| topic | tick microbiome <i>Ixodes ricinus</i> <i>Ixodes persulcatus</i> <i>Dermacentor reticulatus</i> Latvia |
| url | https://www.mdpi.com/2076-2607/11/8/1970 |
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