Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity
Deoxynivalenol (DON) is a well-known mycotoxin, responsible for outbreaks of gastrointestinal disorders in Japan. Fusarium graminearum, a parasite of cereal crops, produces this toxin and this is one of the reasons why it is important to understand its metabolism. It is possible to predict the mold&...
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MDPI AG
2018-12-01
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| Series: | Foods |
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| Online Access: | http://www.mdpi.com/2304-8158/8/1/7 |
| _version_ | 1828730759832141824 |
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| author | Edgar Cambaza Shigenobu Koseki Shuso Kawamura |
| author_facet | Edgar Cambaza Shigenobu Koseki Shuso Kawamura |
| author_sort | Edgar Cambaza |
| collection | DOAJ |
| description | Deoxynivalenol (DON) is a well-known mycotoxin, responsible for outbreaks of gastrointestinal disorders in Japan. Fusarium graminearum, a parasite of cereal crops, produces this toxin and this is one of the reasons why it is important to understand its metabolism. It is possible to predict the mold’s color change and the quantity of DON synthesized throughout its lifecycle. Furthermore, aw has been found to affect the amount of DON. This study aimed to analyze the potential of F. graminearum surface color as a predictor of DON concentration at aw = 0.94, 0.97, and 0.99. Thus, 36 specimens were incubated at 25 °C, 12 at each aw. After 4, 8, 12, and 16 days, three specimens from each aw were collected for color analysis and DON quantification. For color analysis, photos were taken and red, green and blue (RGB) channels were measured on ImageJ software. DON was quantified through liquid chromatography (HPLC). Color changes were only observed at aw = 0.99 because at lower aw the molds presented high growth of white mycelium. Yet, DON increased in all cases. It was only possible to relate the colors with DON concentration at aw = 0.99, where they presented inverse proportionality. |
| first_indexed | 2024-04-12T17:35:52Z |
| format | Article |
| id | doaj.art-75cee66ac9044890ad9406d08bf9384d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-12T17:35:52Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-75cee66ac9044890ad9406d08bf9384d2022-12-22T03:22:59ZengMDPI AGFoods2304-81582018-12-0181710.3390/foods8010007foods8010007Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water ActivityEdgar Cambaza0Shigenobu Koseki1Shuso Kawamura2Laboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-0808, JapanLaboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-0808, JapanLaboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-0808, JapanDeoxynivalenol (DON) is a well-known mycotoxin, responsible for outbreaks of gastrointestinal disorders in Japan. Fusarium graminearum, a parasite of cereal crops, produces this toxin and this is one of the reasons why it is important to understand its metabolism. It is possible to predict the mold’s color change and the quantity of DON synthesized throughout its lifecycle. Furthermore, aw has been found to affect the amount of DON. This study aimed to analyze the potential of F. graminearum surface color as a predictor of DON concentration at aw = 0.94, 0.97, and 0.99. Thus, 36 specimens were incubated at 25 °C, 12 at each aw. After 4, 8, 12, and 16 days, three specimens from each aw were collected for color analysis and DON quantification. For color analysis, photos were taken and red, green and blue (RGB) channels were measured on ImageJ software. DON was quantified through liquid chromatography (HPLC). Color changes were only observed at aw = 0.99 because at lower aw the molds presented high growth of white mycelium. Yet, DON increased in all cases. It was only possible to relate the colors with DON concentration at aw = 0.99, where they presented inverse proportionality.http://www.mdpi.com/2304-8158/8/1/7Fusarium graminearumdeoxynivalenolRGBwater activity |
| spellingShingle | Edgar Cambaza Shigenobu Koseki Shuso Kawamura Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity Foods Fusarium graminearum deoxynivalenol RGB water activity |
| title | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
| title_full | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
| title_fullStr | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
| title_full_unstemmed | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
| title_short | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
| title_sort | fusarium graminearum colors and deoxynivalenol synthesis at different water activity |
| topic | Fusarium graminearum deoxynivalenol RGB water activity |
| url | http://www.mdpi.com/2304-8158/8/1/7 |
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