Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment
Minocycline hydrochloride (MINO) has been one of the most frequently used antibiotics in the treatment of periodontitis due to its antibacterial activity and osteogenesis effects; however, high levels of MINO administered during the treatment halt the formation of new bone. Therefore, the purpose of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Drug Delivery |
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| Online Access: | http://dx.doi.org/10.1080/10717544.2021.1902020 |
| _version_ | 1819279596646825984 |
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| author | Ting Zhang Yingqian Qiu Jinlin Song Pengfei Zhou Hang Liao Yuting Cheng Xiaohong Wu |
| author_facet | Ting Zhang Yingqian Qiu Jinlin Song Pengfei Zhou Hang Liao Yuting Cheng Xiaohong Wu |
| author_sort | Ting Zhang |
| collection | DOAJ |
| description | Minocycline hydrochloride (MINO) has been one of the most frequently used antibiotics in the treatment of periodontitis due to its antibacterial activity and osteogenesis effects; however, high levels of MINO administered during the treatment halt the formation of new bone. Therefore, the purpose of the present study was to prepare a MINO-microsphere/sucrose acetate isobutyrate (SAIB) hybrid depot to reduce the burst release of MINO and ensure antibacterial and osteogenesis effects of MINO in the treatment of periodontitis. Uniform microspheres, approximately 5 µm size, with a slightly rough surface and different MINO loading (10, 12, and 14%) were prepared, and the microspheres were added into SAIB, after which the burst release significantly decreased from 66.18 to 2.92%, from 71.82 to 3.82%, and from 73.35 to 4.45%, respectively, and the release from all the MINO-microspheres/SAIB hybrid depots lasted for 77 days. In addition, cytotoxicity test showed that the MINO-microsphere with 12% drug loading promoted the proliferation of osteoblasts the most and was subsequently used in vivo experiments. Moreover, in the model of ligatured-induced periodontitis in SD rats, the MINO-microsphere/SAIB hybrid depot not only significantly increased the alveolar bone height and bone volume but also reduced the inflammation of the periodontal tissue. Additionally, it also inhibited the expression of the receptor activator of nuclear factor-kappa B ligand (RANKL) and promoted the expression of osteoprotegerin (OPG).. These results indicated that the MINO-microsphere/SAIB hybrid depot might be promising in the treatment of periodontitis. |
| first_indexed | 2024-12-24T00:30:25Z |
| format | Article |
| id | doaj.art-75d17f9a404240cb87a18fafc4c4acbc |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-24T00:30:25Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-75d17f9a404240cb87a18fafc4c4acbc2022-12-21T17:24:17ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642021-01-0128162063310.1080/10717544.2021.19020201902020Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatmentTing Zhang0Yingqian Qiu1Jinlin Song2Pengfei Zhou3Hang Liao4Yuting Cheng5Xiaohong Wu6Stomatological Hospital of Chongqing Medical UniversityStomatological Hospital of Chongqing Medical UniversityStomatological Hospital of Chongqing Medical UniversityStomatological Hospital of Chongqing Medical UniversityStomatological Hospital of Chongqing Medical UniversityStomatological Hospital of Chongqing Medical UniversityStomatological Hospital of Chongqing Medical UniversityMinocycline hydrochloride (MINO) has been one of the most frequently used antibiotics in the treatment of periodontitis due to its antibacterial activity and osteogenesis effects; however, high levels of MINO administered during the treatment halt the formation of new bone. Therefore, the purpose of the present study was to prepare a MINO-microsphere/sucrose acetate isobutyrate (SAIB) hybrid depot to reduce the burst release of MINO and ensure antibacterial and osteogenesis effects of MINO in the treatment of periodontitis. Uniform microspheres, approximately 5 µm size, with a slightly rough surface and different MINO loading (10, 12, and 14%) were prepared, and the microspheres were added into SAIB, after which the burst release significantly decreased from 66.18 to 2.92%, from 71.82 to 3.82%, and from 73.35 to 4.45%, respectively, and the release from all the MINO-microspheres/SAIB hybrid depots lasted for 77 days. In addition, cytotoxicity test showed that the MINO-microsphere with 12% drug loading promoted the proliferation of osteoblasts the most and was subsequently used in vivo experiments. Moreover, in the model of ligatured-induced periodontitis in SD rats, the MINO-microsphere/SAIB hybrid depot not only significantly increased the alveolar bone height and bone volume but also reduced the inflammation of the periodontal tissue. Additionally, it also inhibited the expression of the receptor activator of nuclear factor-kappa B ligand (RANKL) and promoted the expression of osteoprotegerin (OPG).. These results indicated that the MINO-microsphere/SAIB hybrid depot might be promising in the treatment of periodontitis.http://dx.doi.org/10.1080/10717544.2021.1902020periodontitisminocycline hydrochloridesucrose acetate isobutyratedrug releaseosteogenesis |
| spellingShingle | Ting Zhang Yingqian Qiu Jinlin Song Pengfei Zhou Hang Liao Yuting Cheng Xiaohong Wu Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment Drug Delivery periodontitis minocycline hydrochloride sucrose acetate isobutyrate drug release osteogenesis |
| title | Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment |
| title_full | Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment |
| title_fullStr | Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment |
| title_full_unstemmed | Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment |
| title_short | Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment |
| title_sort | electrosprayed minocycline hydrochloride loaded microsphere saib hybrid depot for periodontitis treatment |
| topic | periodontitis minocycline hydrochloride sucrose acetate isobutyrate drug release osteogenesis |
| url | http://dx.doi.org/10.1080/10717544.2021.1902020 |
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