| Summary: | Abstract Brazil’s Midwest is composed of four biomes: the Cerrado, predominant in the region; the Pantanal, the largest wetland in the world; the Amazon, which occupies part of Mato Grosso; and the Atlantic Forest. The objective of this study was to identify the evolution of occupation and use of land in the rural settlements of the Brazilian Midwest depending on the biome of location. A total of 54 settlements distributed in the four biomes of the region were analyzed using direct observation and Landsat images from the years 2004 and 2014. Using the software QGIS 2.8 Wien, the vegetation indices NDVI and NDWI were used to classify agricultural, pasture and forest areas by biome. Native vegetation is declining in most of the analyzed settlements and pastures, for milk production, occupied the largest area. Between 2004 and 2014, pasture areas expanded to the detriment of forests. Although they have the highest percentage of environmental preservation areas, the settlements we analyzed in the Amazon biome do not comply with legislation. Part of the forest in these settlements was transformed into areas of bushy cerrado. However, there was an increase in forests in the settlements of the Atlantic Forest biome.
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