Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates
Curcumin (CUR), a polyphenolic substance extracted from plants, has extensive pharmacological activities. However, CUR is difficult to be absorbed in the body due to its poor stability and low solubility. Studies have found that cochleates can be used as a new delivery system to encapsulate bioactiv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/5/710 |
| _version_ | 1797475074160197632 |
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| author | Lijuan Chen Bowen Yue Zhiming Liu Yali Luo Lu Ni Zhiyong Zhou Xuemei Ge |
| author_facet | Lijuan Chen Bowen Yue Zhiming Liu Yali Luo Lu Ni Zhiyong Zhou Xuemei Ge |
| author_sort | Lijuan Chen |
| collection | DOAJ |
| description | Curcumin (CUR), a polyphenolic substance extracted from plants, has extensive pharmacological activities. However, CUR is difficult to be absorbed in the body due to its poor stability and low solubility. Studies have found that cochleates can be used as a new delivery system to encapsulate bioactive agents for the purpose of improving its stability and bioavailability. In this study, thin-film dispersion and trapping methods were used to prepare curcumin-loaded cochleates (CUR-Cochs). Then CUR-Cochs were characterized and the encapsulation efficiency was determined by HPLC. In addition, the freeze-drying process of CUR-Cochs was studied and related characterization was performed. CCK-8 assay was used to detect the cytotoxicity of cochleates carrier. Additionally, H<sub>2</sub>O<sub>2</sub>-induced cellular oxidative damage model were used to evaluate its antioxidant capacity. The results showed that the structure of CUR-Cochs was a spiral cylinder with an average particle size of 463.8 nm and zeta potential of −15.47 mV. The encapsulation efficiency was the highest (83.66 ± 0.8)% with 1:50 CUR-to-lipid mass ratio. In vitro results showed that cochleates had negligible cytotoxicity and owned antioxidant capacity, which provided the possibility for their applications in food and medicine. In general, the method herein might be a promising method to encapsulate CUR for further use as a bioactive agent in functional foods. |
| first_indexed | 2024-03-09T20:39:54Z |
| format | Article |
| id | doaj.art-75d3219cac4d4cb1afdd7fcc4da02150 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T20:39:54Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-75d3219cac4d4cb1afdd7fcc4da021502023-11-23T23:01:18ZengMDPI AGFoods2304-81582022-02-0111571010.3390/foods11050710Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded CochleatesLijuan Chen0Bowen Yue1Zhiming Liu2Yali Luo3Lu Ni4Zhiyong Zhou5Xuemei Ge6Department of Food Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Pharmacy, Medical College of China Three Gorges University, Yichang 443002, ChinaDepartment of Food Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Food Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Pharmacy, Medical College of China Three Gorges University, Yichang 443002, ChinaDepartment of Pharmacy, Medical College of China Three Gorges University, Yichang 443002, ChinaDepartment of Food Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, ChinaCurcumin (CUR), a polyphenolic substance extracted from plants, has extensive pharmacological activities. However, CUR is difficult to be absorbed in the body due to its poor stability and low solubility. Studies have found that cochleates can be used as a new delivery system to encapsulate bioactive agents for the purpose of improving its stability and bioavailability. In this study, thin-film dispersion and trapping methods were used to prepare curcumin-loaded cochleates (CUR-Cochs). Then CUR-Cochs were characterized and the encapsulation efficiency was determined by HPLC. In addition, the freeze-drying process of CUR-Cochs was studied and related characterization was performed. CCK-8 assay was used to detect the cytotoxicity of cochleates carrier. Additionally, H<sub>2</sub>O<sub>2</sub>-induced cellular oxidative damage model were used to evaluate its antioxidant capacity. The results showed that the structure of CUR-Cochs was a spiral cylinder with an average particle size of 463.8 nm and zeta potential of −15.47 mV. The encapsulation efficiency was the highest (83.66 ± 0.8)% with 1:50 CUR-to-lipid mass ratio. In vitro results showed that cochleates had negligible cytotoxicity and owned antioxidant capacity, which provided the possibility for their applications in food and medicine. In general, the method herein might be a promising method to encapsulate CUR for further use as a bioactive agent in functional foods.https://www.mdpi.com/2304-8158/11/5/710curcuminlipidcochleatescharacterizationstability |
| spellingShingle | Lijuan Chen Bowen Yue Zhiming Liu Yali Luo Lu Ni Zhiyong Zhou Xuemei Ge Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates Foods curcumin lipid cochleates characterization stability |
| title | Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates |
| title_full | Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates |
| title_fullStr | Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates |
| title_full_unstemmed | Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates |
| title_short | Study on the Preparation, Characterization, and Stability of Freeze-Dried Curcumin-Loaded Cochleates |
| title_sort | study on the preparation characterization and stability of freeze dried curcumin loaded cochleates |
| topic | curcumin lipid cochleates characterization stability |
| url | https://www.mdpi.com/2304-8158/11/5/710 |
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