Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis
The present experiment was conducted to study variation in milk California mastitis test(CMT) white side test(WST) and chloride test, pH test, along with activities of whey enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in relation to staphylococ...
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| Format: | Article |
| Language: | English |
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Mirror of Research in Veterinary Sciences and Animals (MRVSA)
2013-07-01
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| Series: | Mirror of Research in Veterinary Sciences and Animals |
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| Online Access: | http://mrvsa.com/upload/article%202%20Alteration%20of%20some%20enzymatic%20activities%20in%20whey%20of%20ewe%E2%80%99s%20milk%20.%20-%20Copy.pdf |
| _version_ | 1818050460369551360 |
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| author | Mustafa Salah Hassan Afaf AbdulrahmanYousif |
| author_facet | Mustafa Salah Hassan Afaf AbdulrahmanYousif |
| author_sort | Mustafa Salah Hassan |
| collection | DOAJ |
| description | The present experiment was conducted to study variation in milk California mastitis test(CMT) white side test(WST) and chloride test, pH test, along with activities of whey enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in relation to staphylococcal mastitis in lactating ewes. A total of 310 milk samples were collected from the udder halves of 161 dairy ewes at mid period of lactation to determine the percentage of Staphylococcus mastitis. The overall percentage of infection with clinical and subclinical Staphylococcal mastitis was found to be 2.25 % and 12.22% respectively. All samples were subjected to bacteriological examination and the following staphylococcal species were isolated, coagulase negative Staphylococcus (1.29% & 27.8%) and Staphylococcus aureus (27.8% &12.22%) from clinical and subclinical mastitis respectively. The whey samples were divided into three groups: a non-infected group, subclinical infected group and clinical infected group for estimation of enzymes. Activities of LDH, ALP and AST were significantly higher in milk from the subclinical and clinical mastitis groups for S. aureus and coagulase negative Staphylococcus(CNS) (AST:222.09±31.54;194±27.15&271.82±30.50;201.0±49.51;ALP:837.08±63.57; 866.01±215.36& 884.22±26.08 ;807.45± 47.05LDH:332.95±5.67& 289.83±32.95;344.2 ±21.17 ;307.62± 72.77) respectively, than in non-infected group(AST: 38.84±2.71; ALP: 187.91±5.54; LDH: 142.59± 5.67).
In conclusions, the results of the present study showed that the measurement of AST, LDH and ALP activities in milk samples could be used as reliable method and suitable for detection of ovine subclinical mastitis. |
| first_indexed | 2024-12-10T10:53:49Z |
| format | Article |
| id | doaj.art-75d54060c8af4228a0f8a1fc89adb701 |
| institution | Directory Open Access Journal |
| issn | 2520-324X 2520-324X |
| language | English |
| last_indexed | 2024-12-10T10:53:49Z |
| publishDate | 2013-07-01 |
| publisher | Mirror of Research in Veterinary Sciences and Animals (MRVSA) |
| record_format | Article |
| series | Mirror of Research in Veterinary Sciences and Animals |
| spelling | doaj.art-75d54060c8af4228a0f8a1fc89adb7012022-12-22T01:51:56ZengMirror of Research in Veterinary Sciences and Animals (MRVSA)Mirror of Research in Veterinary Sciences and Animals2520-324X2520-324X2013-07-012281510.22428/mrvsa. 2307-8073.2013. 00222.xAlteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitisMustafa Salah Hassan 0Afaf AbdulrahmanYousif1Dept. of Vet Internal and Preventive Medicine / College of Veterinary Medicine/ University of Baghdad, Iraq Dept. of Vet Internal and Preventive Medicine / College of Veterinary Medicine/ University of Baghdad, Iraq The present experiment was conducted to study variation in milk California mastitis test(CMT) white side test(WST) and chloride test, pH test, along with activities of whey enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in relation to staphylococcal mastitis in lactating ewes. A total of 310 milk samples were collected from the udder halves of 161 dairy ewes at mid period of lactation to determine the percentage of Staphylococcus mastitis. The overall percentage of infection with clinical and subclinical Staphylococcal mastitis was found to be 2.25 % and 12.22% respectively. All samples were subjected to bacteriological examination and the following staphylococcal species were isolated, coagulase negative Staphylococcus (1.29% & 27.8%) and Staphylococcus aureus (27.8% &12.22%) from clinical and subclinical mastitis respectively. The whey samples were divided into three groups: a non-infected group, subclinical infected group and clinical infected group for estimation of enzymes. Activities of LDH, ALP and AST were significantly higher in milk from the subclinical and clinical mastitis groups for S. aureus and coagulase negative Staphylococcus(CNS) (AST:222.09±31.54;194±27.15&271.82±30.50;201.0±49.51;ALP:837.08±63.57; 866.01±215.36& 884.22±26.08 ;807.45± 47.05LDH:332.95±5.67& 289.83±32.95;344.2 ±21.17 ;307.62± 72.77) respectively, than in non-infected group(AST: 38.84±2.71; ALP: 187.91±5.54; LDH: 142.59± 5.67). In conclusions, the results of the present study showed that the measurement of AST, LDH and ALP activities in milk samples could be used as reliable method and suitable for detection of ovine subclinical mastitis.http://mrvsa.com/upload/article%202%20Alteration%20of%20some%20enzymatic%20activities%20in%20whey%20of%20ewe%E2%80%99s%20milk%20.%20-%20Copy.pdfLactate dehydrogenaseAlkaline phosphataseSubclinical mastitisclinical mastitis |
| spellingShingle | Mustafa Salah Hassan Afaf AbdulrahmanYousif Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis Mirror of Research in Veterinary Sciences and Animals Lactate dehydrogenase Alkaline phosphatase Subclinical mastitis clinical mastitis |
| title | Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis |
| title_full | Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis |
| title_fullStr | Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis |
| title_full_unstemmed | Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis |
| title_short | Alteration of some enzymatic activities in whey of ewe’s milk Suffered from Staphylococcal mastitis |
| title_sort | alteration of some enzymatic activities in whey of ewe s milk suffered from staphylococcal mastitis |
| topic | Lactate dehydrogenase Alkaline phosphatase Subclinical mastitis clinical mastitis |
| url | http://mrvsa.com/upload/article%202%20Alteration%20of%20some%20enzymatic%20activities%20in%20whey%20of%20ewe%E2%80%99s%20milk%20.%20-%20Copy.pdf |
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