Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction
Temporomandibular joint dysfunction (TMD) is a multifactorial condition that impairs human’s health and quality of life. Its etiology is still a challenge due to its complex development and the great number of different conditions it comprises. One of the most common forms of TMD is anterior disc di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/4/601 |
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| author | Andrea Duarte Doetzer Roberto Hirochi Herai Marília Afonso Rabelo Buzalaf Paula Cristina Trevilatto |
| author_facet | Andrea Duarte Doetzer Roberto Hirochi Herai Marília Afonso Rabelo Buzalaf Paula Cristina Trevilatto |
| author_sort | Andrea Duarte Doetzer |
| collection | DOAJ |
| description | Temporomandibular joint dysfunction (TMD) is a multifactorial condition that impairs human’s health and quality of life. Its etiology is still a challenge due to its complex development and the great number of different conditions it comprises. One of the most common forms of TMD is anterior disc displacement without reduction (DDWoR) and other TMDs with distinct origins are condylar hyperplasia (CH) and mandibular dislocation (MD). Thus, the aim of this study is to identify the protein expression profile of synovial fluid and the temporomandibular joint disc of patients diagnosed with DDWoR, CH and MD. Synovial fluid and a fraction of the temporomandibular joint disc were collected from nine patients diagnosed with DDWoR (<i>n</i> = 3), CH (<i>n</i> = 4) and MD (<i>n</i> = 2). Samples were subjected to label-free nLC-MS/MS for proteomic data extraction, and then bioinformatics analysis were conducted for protein identification and functional annotation. The three TMD conditions showed different protein expression profiles, and novel proteins were identified in both synovial fluid and disc sample. TMD is a complex condition and the identification of the proteins expressed in the three different types of TMD may contribute to a better comprehension of how each pathology develops and evolutes, benefitting the patient with a focus–target treatment. |
| first_indexed | 2024-03-10T12:50:23Z |
| format | Article |
| id | doaj.art-75d66fb94efb4604a4b989f1ad24b7f1 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T12:50:23Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-75d66fb94efb4604a4b989f1ad24b7f12023-11-21T13:08:27ZengMDPI AGDiagnostics2075-44182021-03-0111460110.3390/diagnostics11040601Proteomic Expression Profile in Human Temporomandibular Joint DysfunctionAndrea Duarte Doetzer0Roberto Hirochi Herai1Marília Afonso Rabelo Buzalaf2Paula Cristina Trevilatto3Graduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba 80215-901, BrazilGraduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba 80215-901, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, BrazilGraduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba 80215-901, BrazilTemporomandibular joint dysfunction (TMD) is a multifactorial condition that impairs human’s health and quality of life. Its etiology is still a challenge due to its complex development and the great number of different conditions it comprises. One of the most common forms of TMD is anterior disc displacement without reduction (DDWoR) and other TMDs with distinct origins are condylar hyperplasia (CH) and mandibular dislocation (MD). Thus, the aim of this study is to identify the protein expression profile of synovial fluid and the temporomandibular joint disc of patients diagnosed with DDWoR, CH and MD. Synovial fluid and a fraction of the temporomandibular joint disc were collected from nine patients diagnosed with DDWoR (<i>n</i> = 3), CH (<i>n</i> = 4) and MD (<i>n</i> = 2). Samples were subjected to label-free nLC-MS/MS for proteomic data extraction, and then bioinformatics analysis were conducted for protein identification and functional annotation. The three TMD conditions showed different protein expression profiles, and novel proteins were identified in both synovial fluid and disc sample. TMD is a complex condition and the identification of the proteins expressed in the three different types of TMD may contribute to a better comprehension of how each pathology develops and evolutes, benefitting the patient with a focus–target treatment.https://www.mdpi.com/2075-4418/11/4/601temporomandibular jointprotein expressiontemporomandibular joint dysfunction |
| spellingShingle | Andrea Duarte Doetzer Roberto Hirochi Herai Marília Afonso Rabelo Buzalaf Paula Cristina Trevilatto Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction Diagnostics temporomandibular joint protein expression temporomandibular joint dysfunction |
| title | Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction |
| title_full | Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction |
| title_fullStr | Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction |
| title_full_unstemmed | Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction |
| title_short | Proteomic Expression Profile in Human Temporomandibular Joint Dysfunction |
| title_sort | proteomic expression profile in human temporomandibular joint dysfunction |
| topic | temporomandibular joint protein expression temporomandibular joint dysfunction |
| url | https://www.mdpi.com/2075-4418/11/4/601 |
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