Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform
Engineering enzymes to utilize the noncanonical redox cofactors such as nicotinamide mononucleotide (NMN + ) is challenging. Here, the authors report a growth-based selection platform for NMN + -reducing enzyme engineering and show its application in developing a phosphite dehydrogenase with improve...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32727-w |
| _version_ | 1811281159696416768 |
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| author | Linyue Zhang Edward King William B. Black Christian M. Heckmann Allison Wolder Youtian Cui Francis Nicklen Justin B. Siegel Ray Luo Caroline E. Paul Han Li |
| author_facet | Linyue Zhang Edward King William B. Black Christian M. Heckmann Allison Wolder Youtian Cui Francis Nicklen Justin B. Siegel Ray Luo Caroline E. Paul Han Li |
| author_sort | Linyue Zhang |
| collection | DOAJ |
| description | Engineering enzymes to utilize the noncanonical redox cofactors such as nicotinamide mononucleotide (NMN + ) is challenging. Here, the authors report a growth-based selection platform for NMN + -reducing enzyme engineering and show its application in developing a phosphite dehydrogenase with improved catalytic efficiency. |
| first_indexed | 2024-04-13T01:27:43Z |
| format | Article |
| id | doaj.art-75d6e8408c81418bb081b0cb20fbd5ad |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T01:27:43Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-75d6e8408c81418bb081b0cb20fbd5ad2022-12-22T03:08:35ZengNature PortfolioNature Communications2041-17232022-08-0113111210.1038/s41467-022-32727-wDirected evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platformLinyue Zhang0Edward King1William B. Black2Christian M. Heckmann3Allison Wolder4Youtian Cui5Francis Nicklen6Justin B. Siegel7Ray Luo8Caroline E. Paul9Han Li10Department of Chemical and Biomolecular Engineering, University of California IrvineDepartment of Molecular Biology and Biochemistry, University of California IrvineDepartment of Chemical and Biomolecular Engineering, University of California IrvineBiocatalysis, Department of Biotechnology, Delft University of TechnologyBiocatalysis, Department of Biotechnology, Delft University of TechnologyDepartment of Chemistry, University of California, Davis, One Shields AvenueDepartment of Chemical and Biomolecular Engineering, University of California IrvineDepartment of Chemistry, University of California, Davis, One Shields AvenueDepartment of Chemical and Biomolecular Engineering, University of California IrvineBiocatalysis, Department of Biotechnology, Delft University of TechnologyDepartment of Chemical and Biomolecular Engineering, University of California IrvineEngineering enzymes to utilize the noncanonical redox cofactors such as nicotinamide mononucleotide (NMN + ) is challenging. Here, the authors report a growth-based selection platform for NMN + -reducing enzyme engineering and show its application in developing a phosphite dehydrogenase with improved catalytic efficiency.https://doi.org/10.1038/s41467-022-32727-w |
| spellingShingle | Linyue Zhang Edward King William B. Black Christian M. Heckmann Allison Wolder Youtian Cui Francis Nicklen Justin B. Siegel Ray Luo Caroline E. Paul Han Li Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform Nature Communications |
| title | Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform |
| title_full | Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform |
| title_fullStr | Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform |
| title_full_unstemmed | Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform |
| title_short | Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform |
| title_sort | directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform |
| url | https://doi.org/10.1038/s41467-022-32727-w |
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