Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme
A new strategy for the design and construction of molecularly imprinted magnetic fluorescent nanocomposite-based-sensor is proposed. This multifunctional nanocomposite exhibits the necessary optics, magnetism and biocompatibility for use in the selective fluorescence detection of lysozyme. The magne...
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MDPI AG
2021-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/6/1575 |
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| author | Xin Zhang Bo Tang Yansong Li Chengbin Liu Pengfei Jiao Yuping Wei |
| author_facet | Xin Zhang Bo Tang Yansong Li Chengbin Liu Pengfei Jiao Yuping Wei |
| author_sort | Xin Zhang |
| collection | DOAJ |
| description | A new strategy for the design and construction of molecularly imprinted magnetic fluorescent nanocomposite-based-sensor is proposed. This multifunctional nanocomposite exhibits the necessary optics, magnetism and biocompatibility for use in the selective fluorescence detection of lysozyme. The magnetic fluorescent nanocomposites are prepared by combining carboxyl- functionalized Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles with <i>l</i>-cysteine-modified zinc sulfide quantum dots (MNP/QDs). Surface molecular imprinting technology was employed to coat the lysozyme molecularly imprinted polymer (MIP) layer on the MNP/QDs to form a core-shell structure. The molecularly imprinted MNP/QDs (MNP/QD@MIPs) can rapidly separate the target protein and then use fluorescence sensing to detect the protein; this reduces the background interference, and the selectivity and sensitivity of the detection are improved. The molecularly imprinted MNP/QDs sensor presented good linearity over a lysozyme concentration range from 0.2 to 2.0 μM and a detection limit of 4.53 × 10<sup>−3</sup> μM for lysozyme. The imprinting factor of the MNP/QD@MIPs was 4.12, and the selectivity coefficient ranged from 3.19 to 3.85. Furthermore, the MNP/QD@MIPs sensor was applied to detect of lysozyme in human urine and egg white samples with recoveries of 95.40–103.33%. Experimental results showed that the prepared MNP/QD@MIPs has potential for selective magnetic separation and fluorescence sensing of target proteins in biological samples. |
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| id | doaj.art-75d77cc9c21c4896adc6115cec5e0362 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T10:23:32Z |
| publishDate | 2021-06-01 |
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| series | Nanomaterials |
| spelling | doaj.art-75d77cc9c21c4896adc6115cec5e03622023-11-22T00:13:08ZengMDPI AGNanomaterials2079-49912021-06-01116157510.3390/nano11061575Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of LysozymeXin Zhang0Bo Tang1Yansong Li2Chengbin Liu3Pengfei Jiao4Yuping Wei5School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, ChinaKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, ChinaSchool of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, ChinaSchool of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, ChinaSchool of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, ChinaA new strategy for the design and construction of molecularly imprinted magnetic fluorescent nanocomposite-based-sensor is proposed. This multifunctional nanocomposite exhibits the necessary optics, magnetism and biocompatibility for use in the selective fluorescence detection of lysozyme. The magnetic fluorescent nanocomposites are prepared by combining carboxyl- functionalized Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles with <i>l</i>-cysteine-modified zinc sulfide quantum dots (MNP/QDs). Surface molecular imprinting technology was employed to coat the lysozyme molecularly imprinted polymer (MIP) layer on the MNP/QDs to form a core-shell structure. The molecularly imprinted MNP/QDs (MNP/QD@MIPs) can rapidly separate the target protein and then use fluorescence sensing to detect the protein; this reduces the background interference, and the selectivity and sensitivity of the detection are improved. The molecularly imprinted MNP/QDs sensor presented good linearity over a lysozyme concentration range from 0.2 to 2.0 μM and a detection limit of 4.53 × 10<sup>−3</sup> μM for lysozyme. The imprinting factor of the MNP/QD@MIPs was 4.12, and the selectivity coefficient ranged from 3.19 to 3.85. Furthermore, the MNP/QD@MIPs sensor was applied to detect of lysozyme in human urine and egg white samples with recoveries of 95.40–103.33%. Experimental results showed that the prepared MNP/QD@MIPs has potential for selective magnetic separation and fluorescence sensing of target proteins in biological samples.https://www.mdpi.com/2079-4991/11/6/1575molecular imprintingmagnetic nanoparticlesquantum dotsfluorescent sensor |
| spellingShingle | Xin Zhang Bo Tang Yansong Li Chengbin Liu Pengfei Jiao Yuping Wei Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme Nanomaterials molecular imprinting magnetic nanoparticles quantum dots fluorescent sensor |
| title | Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme |
| title_full | Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme |
| title_fullStr | Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme |
| title_full_unstemmed | Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme |
| title_short | Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme |
| title_sort | molecularly imprinted magnetic fluorescent nanocomposite based sensor for selective detection of lysozyme |
| topic | molecular imprinting magnetic nanoparticles quantum dots fluorescent sensor |
| url | https://www.mdpi.com/2079-4991/11/6/1575 |
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