Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity
Bioactive compounds extracted from marine organisms showed several biological activities. The present study is an extension of our earlier studies where we assessed the antiproliferative and pro-apoptotic activities of ethanol, methylene chloride, ethyl acetate, acetone, and chloroform crude extract...
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2022-01-01
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| author | Hussein A. El-Naggar Mansour A. E. Bashar Islam Rady Mohammad S. El-Wetidy Waleed B. Suleiman Fatimah O. Al-Otibi Sara A. Al-Rashed Lamiaa M. Abd El-Maoula El-Sayed S. Salem Enas M. H. Attia Sayed Bakry |
| author_facet | Hussein A. El-Naggar Mansour A. E. Bashar Islam Rady Mohammad S. El-Wetidy Waleed B. Suleiman Fatimah O. Al-Otibi Sara A. Al-Rashed Lamiaa M. Abd El-Maoula El-Sayed S. Salem Enas M. H. Attia Sayed Bakry |
| author_sort | Hussein A. El-Naggar |
| collection | DOAJ |
| description | Bioactive compounds extracted from marine organisms showed several biological activities. The present study is an extension of our earlier studies where we assessed the antiproliferative and pro-apoptotic activities of ethanol, methylene chloride, ethyl acetate, acetone, and chloroform crude extracts of sponges: <i>Negombata magnifica</i> (<i>Nm</i>E) and <i>Callyspongia siphonella</i> (<i>Cs</i>E) against cancer cells. Herein, we are extending our previous findings on both sponge species depending on an alternative methanol extraction method with more advanced molecular biochemical insights as additional proof for anticancer and antimicrobial activity of <i>N. magnifica</i> and <i>C. siphonella</i>. Therefore, sponge specimens were collected during winter 2020 from the Dahab region at the Gulf of Aqaba. Each sponge was macerated with methanol to obtain the crude extracts; <i>Nm</i>E and <i>Cs</i>E. GC–MS analysis presented a total of 117 chemical compounds; 37 bioactive, 11 represented previously as constituents for a natural organism, and 69 had no biological activities. <i>Nm</i>E dose-dependently inhibited the growth of HepG2, MCF-7, and Caco-2 carcinoma cell lines compared to <i>Cs</i>E, which unfortunately has no antiproliferative activity against the same cancer cells. <i>Nm</i>E was found to induce G0/G1 cell cycle arrest in HepG2 cells with its inhibition for CDK6, Cyclins D1, and E1 in HepG2, MCF-7, and Caco-2 cells. <i>Nm</i>E also activated ROS production in HepG2 cells and induced apoptosis in HepG2, MCF-7, and Caco-2 cells via an increase in pro-apoptotic protein Bax, caspase-3, and cleavage PARP, and a decrease in anti-apoptotic protein BCL2. Unlike its anticancer potential, <i>Cs</i>E exhibited clear superior results as an antimicrobial agent with a wider range against six microbial strains, whereas <i>Nm</i>E showed a positive antibacterial activity against only two strains. |
| first_indexed | 2024-03-10T00:13:08Z |
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| last_indexed | 2024-03-10T00:13:08Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-75d7edd987ee473f8cd67e774b8d7cce2023-11-23T15:57:02ZengMDPI AGApplied Sciences2076-34172022-01-01123140010.3390/app12031400Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial ActivityHussein A. El-Naggar0Mansour A. E. Bashar1Islam Rady2Mohammad S. El-Wetidy3Waleed B. Suleiman4Fatimah O. Al-Otibi5Sara A. Al-Rashed6Lamiaa M. Abd El-Maoula7El-Sayed S. Salem8Enas M. H. Attia9Sayed Bakry10Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptZoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptZoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptCollege of Medicine Research Center, King Saud University, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaNutrition and Food Science Department, Faculty of Home Economics, Al-Azhar University, Tanta 31732, EgyptEnvironmental Egyptian Affairs Agency, Red Sea Protectorates Sector, Hurghada 84511, EgyptPhysics Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11823, EgyptZoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptBioactive compounds extracted from marine organisms showed several biological activities. The present study is an extension of our earlier studies where we assessed the antiproliferative and pro-apoptotic activities of ethanol, methylene chloride, ethyl acetate, acetone, and chloroform crude extracts of sponges: <i>Negombata magnifica</i> (<i>Nm</i>E) and <i>Callyspongia siphonella</i> (<i>Cs</i>E) against cancer cells. Herein, we are extending our previous findings on both sponge species depending on an alternative methanol extraction method with more advanced molecular biochemical insights as additional proof for anticancer and antimicrobial activity of <i>N. magnifica</i> and <i>C. siphonella</i>. Therefore, sponge specimens were collected during winter 2020 from the Dahab region at the Gulf of Aqaba. Each sponge was macerated with methanol to obtain the crude extracts; <i>Nm</i>E and <i>Cs</i>E. GC–MS analysis presented a total of 117 chemical compounds; 37 bioactive, 11 represented previously as constituents for a natural organism, and 69 had no biological activities. <i>Nm</i>E dose-dependently inhibited the growth of HepG2, MCF-7, and Caco-2 carcinoma cell lines compared to <i>Cs</i>E, which unfortunately has no antiproliferative activity against the same cancer cells. <i>Nm</i>E was found to induce G0/G1 cell cycle arrest in HepG2 cells with its inhibition for CDK6, Cyclins D1, and E1 in HepG2, MCF-7, and Caco-2 cells. <i>Nm</i>E also activated ROS production in HepG2 cells and induced apoptosis in HepG2, MCF-7, and Caco-2 cells via an increase in pro-apoptotic protein Bax, caspase-3, and cleavage PARP, and a decrease in anti-apoptotic protein BCL2. Unlike its anticancer potential, <i>Cs</i>E exhibited clear superior results as an antimicrobial agent with a wider range against six microbial strains, whereas <i>Nm</i>E showed a positive antibacterial activity against only two strains.https://www.mdpi.com/2076-3417/12/3/1400sponges<i>Negombata magnifica</i><i>Callyspongia siphonella</i>Red Seaanticancerantimicrobial |
| spellingShingle | Hussein A. El-Naggar Mansour A. E. Bashar Islam Rady Mohammad S. El-Wetidy Waleed B. Suleiman Fatimah O. Al-Otibi Sara A. Al-Rashed Lamiaa M. Abd El-Maoula El-Sayed S. Salem Enas M. H. Attia Sayed Bakry Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity Applied Sciences sponges <i>Negombata magnifica</i> <i>Callyspongia siphonella</i> Red Sea anticancer antimicrobial |
| title | Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity |
| title_full | Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity |
| title_fullStr | Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity |
| title_full_unstemmed | Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity |
| title_short | Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity |
| title_sort | two red sea sponge extracts i negombata magnifica i and i callyspongia siphonella i induced anticancer and antimicrobial activity |
| topic | sponges <i>Negombata magnifica</i> <i>Callyspongia siphonella</i> Red Sea anticancer antimicrobial |
| url | https://www.mdpi.com/2076-3417/12/3/1400 |
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