Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry
The success of Next generation sequencing (NGS) technology over the past decades has led to a better understanding of complex microbial communities in such environment. Here, we employed NGS to monitor sulfur oxidizing bacteria (SOB) community from cassava starch industry (BPT), which can oxidize H2...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Energy Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352484721005527 |
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| author | Saowaluck Haosagul Sukunya Oaew Peerada Prommeenate Vanatpornratt Sawasdee Siriorn Boonyawanich Nipon Pisutpaisal |
| author_facet | Saowaluck Haosagul Sukunya Oaew Peerada Prommeenate Vanatpornratt Sawasdee Siriorn Boonyawanich Nipon Pisutpaisal |
| author_sort | Saowaluck Haosagul |
| collection | DOAJ |
| description | The success of Next generation sequencing (NGS) technology over the past decades has led to a better understanding of complex microbial communities in such environment. Here, we employed NGS to monitor sulfur oxidizing bacteria (SOB) community from cassava starch industry (BPT), which can oxidize H2S into sulfate or elemental sulfur under aerobic conditions. Microbial sludge was collected from two sampling points comprising starting sludge (BPT1) and recirculating sludge (BPT2) of the biotrickling filter. gDNA was extracted from BPT1 and BPT2 samples and removed other contaminations by using the GenepHarM Gel/PCR Kit (Geneaid). DNA concentrations and DNA purity were analyzed by using gel electrophoresis and NanoDrop™ 1000 Spectrophotometer, respectively. Analysis of SOB species using NGS technique was based on the hypervariable regions V4 on the 16S rRNA gene. The comparative genomic hybridization (CGH) microarray probes for detection and identification of SOB species-strain that high expression of soxAXBYZ and fccAB genes in the cassava starch industry. For the recirculating sludge (BPT2), the bacterial communities were dominated by Sulfurimonas (13.7%) followed by Synechococcus (9.4%), Hydrogenophaga (6.5%), Methanosaeta (2.4%), and Acidithiobacillus (2.1%), the same dominant genera were also found in the starting sludge (BPT1), but with slightly different abundancy. The heatmap revealed that Acidithiobacillus caldus species (33.9%–49.9%) could play a vital role in eliminating H2S in biogas from the cassava starch industry. The CGH microarray indicated that Thiothrix, Paracoccus, Sulfurimonas, Hydrogenophaga, Magnetospirillum, Rhodoplanes, Syntrophomonas, Pseudomonas, Sulfuricurvum, and Arcobacter were dominant genus, that has a high expression of sulfur oxidation genes in biotrickling filter. |
| first_indexed | 2024-12-17T02:13:26Z |
| format | Article |
| id | doaj.art-75d7f1a1cc5d458abcabf8db2dd44b85 |
| institution | Directory Open Access Journal |
| issn | 2352-4847 |
| language | English |
| last_indexed | 2024-12-17T02:13:26Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Energy Reports |
| spelling | doaj.art-75d7f1a1cc5d458abcabf8db2dd44b852022-12-21T22:07:29ZengElsevierEnergy Reports2352-48472021-11-017677685Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industrySaowaluck Haosagul0Sukunya Oaew1Peerada Prommeenate2Vanatpornratt Sawasdee3Siriorn Boonyawanich4Nipon Pisutpaisal5Department of Agro-Industrial, Food and Environment Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand; Biosensor and Bioelectronics Technology Centre, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, ThailandBiochemical Engineering and System Biology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at King Mongkut’s University of Technology Thonburi, Bang Khun Thian, Bangkok, 10150, ThailandBiochemical Engineering and System Biology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at King Mongkut’s University of Technology Thonburi, Bang Khun Thian, Bangkok, 10150, ThailandProgram in Innovation of Environmental Management, College of Innovative Management, Valaya Alongkorn Rajabhat University Under The Royal Patronage Pathumthani 13180, ThailandDepartment of Agro-Industrial, Food and Environment Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand; Biosensor and Bioelectronics Technology Centre, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand; Corresponding author.Department of Agro-Industrial, Food and Environment Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand; Biosensor and Bioelectronics Technology Centre, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand; Corresponding author at: Department of Agro-Industrial, Food and Environment Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand.The success of Next generation sequencing (NGS) technology over the past decades has led to a better understanding of complex microbial communities in such environment. Here, we employed NGS to monitor sulfur oxidizing bacteria (SOB) community from cassava starch industry (BPT), which can oxidize H2S into sulfate or elemental sulfur under aerobic conditions. Microbial sludge was collected from two sampling points comprising starting sludge (BPT1) and recirculating sludge (BPT2) of the biotrickling filter. gDNA was extracted from BPT1 and BPT2 samples and removed other contaminations by using the GenepHarM Gel/PCR Kit (Geneaid). DNA concentrations and DNA purity were analyzed by using gel electrophoresis and NanoDrop™ 1000 Spectrophotometer, respectively. Analysis of SOB species using NGS technique was based on the hypervariable regions V4 on the 16S rRNA gene. The comparative genomic hybridization (CGH) microarray probes for detection and identification of SOB species-strain that high expression of soxAXBYZ and fccAB genes in the cassava starch industry. For the recirculating sludge (BPT2), the bacterial communities were dominated by Sulfurimonas (13.7%) followed by Synechococcus (9.4%), Hydrogenophaga (6.5%), Methanosaeta (2.4%), and Acidithiobacillus (2.1%), the same dominant genera were also found in the starting sludge (BPT1), but with slightly different abundancy. The heatmap revealed that Acidithiobacillus caldus species (33.9%–49.9%) could play a vital role in eliminating H2S in biogas from the cassava starch industry. The CGH microarray indicated that Thiothrix, Paracoccus, Sulfurimonas, Hydrogenophaga, Magnetospirillum, Rhodoplanes, Syntrophomonas, Pseudomonas, Sulfuricurvum, and Arcobacter were dominant genus, that has a high expression of sulfur oxidation genes in biotrickling filter.http://www.sciencedirect.com/science/article/pii/S2352484721005527Biogas clean-upBiotrickling filterHydrogen sulfideCassava starch industrySulfur oxidizing bacteria16S rRNA gene |
| spellingShingle | Saowaluck Haosagul Sukunya Oaew Peerada Prommeenate Vanatpornratt Sawasdee Siriorn Boonyawanich Nipon Pisutpaisal Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry Energy Reports Biogas clean-up Biotrickling filter Hydrogen sulfide Cassava starch industry Sulfur oxidizing bacteria 16S rRNA gene |
| title | Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry |
| title_full | Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry |
| title_fullStr | Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry |
| title_full_unstemmed | Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry |
| title_short | Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry |
| title_sort | profile of sulfur oxidizing bacteria in full scale biotrickling filter to remove h2s in biogas from in cassava starch industry |
| topic | Biogas clean-up Biotrickling filter Hydrogen sulfide Cassava starch industry Sulfur oxidizing bacteria 16S rRNA gene |
| url | http://www.sciencedirect.com/science/article/pii/S2352484721005527 |
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