Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro
Abstract Background Alcohol consumption is a risk factor for impaired fracture healing, though the mechanism(s) by which this occurs are not well understood. Our laboratory has previously shown that episodic alcohol exposure of rodents negatively affects fracture callus development, callus biomechan...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-018-0800-7 |
| _version_ | 1811187841579876352 |
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| author | Roman M. Natoli Henry Yu Megan Conti-Mica Meislin Pegah Abbasnia Philip Roper Aleksandra Vuchkovska Xianghui Xiao Stuart R. Stock John J. Callaci |
| author_facet | Roman M. Natoli Henry Yu Megan Conti-Mica Meislin Pegah Abbasnia Philip Roper Aleksandra Vuchkovska Xianghui Xiao Stuart R. Stock John J. Callaci |
| author_sort | Roman M. Natoli |
| collection | DOAJ |
| description | Abstract Background Alcohol consumption is a risk factor for impaired fracture healing, though the mechanism(s) by which this occurs are not well understood. Our laboratory has previously shown that episodic alcohol exposure of rodents negatively affects fracture callus development, callus biomechanics, and cellular signaling which regulates stem cell differentiation. Here, we examine whether alcohol alters chemokine expression and/or signaling activity in the mouse fracture callus during early fracture healing. Methods A mouse model for alcohol-impaired tibia fracture healing was utilized. Early fracture callus was examined for alcohol-effects on tissue composition, expression of chemokines involved in MSC migration to the fracture site, and biomechanics. The effects of alcohol on MSC migration and cell adhesion receptors were examined in an in vitro system. Results Mice exposed to alcohol showed decreased evidence of external callus formation, decreased callus-related osteopontin (OPN) expression levels, and decreased biomechanical stiffness. Alcohol exposure decreased rOPN-mediated MSC migration and integrin β1 receptor expression in vitro. Conclusions The effects of alcohol exposure demonstrated here on fracture callus-associated OPN expression, rOPN-mediated MSC migration in vitro, and MSC integrin β1 receptor expression in vitro have not been previously reported. Understanding the effects of alcohol exposure on the early stages of fracture repair may allow timely initiation of treatment to mitigate the long-term complications of delayed healing and/or fracture non-union. |
| first_indexed | 2024-04-11T14:10:19Z |
| format | Article |
| id | doaj.art-75d952de1e3d4a53a0b8946100332d03 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T14:10:19Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-75d952de1e3d4a53a0b8946100332d032022-12-22T04:19:45ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-04-0113111310.1186/s13018-018-0800-7Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitroRoman M. Natoli0Henry Yu1Megan Conti-Mica Meislin2Pegah Abbasnia3Philip Roper4Aleksandra Vuchkovska5Xianghui Xiao6Stuart R. Stock7John J. Callaci8Department of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoDepartment of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoDepartment of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoDepartment of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoDepartment of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoDepartment of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoPresent Address: Argonne National Laboratory Advanced Photon SourcePresent Address: School of Medicine, Northwestern University FeinbergDepartment of Orthopaedic Surgery and Rehabilitation, Stritch School of Medicine, Loyola University ChicagoAbstract Background Alcohol consumption is a risk factor for impaired fracture healing, though the mechanism(s) by which this occurs are not well understood. Our laboratory has previously shown that episodic alcohol exposure of rodents negatively affects fracture callus development, callus biomechanics, and cellular signaling which regulates stem cell differentiation. Here, we examine whether alcohol alters chemokine expression and/or signaling activity in the mouse fracture callus during early fracture healing. Methods A mouse model for alcohol-impaired tibia fracture healing was utilized. Early fracture callus was examined for alcohol-effects on tissue composition, expression of chemokines involved in MSC migration to the fracture site, and biomechanics. The effects of alcohol on MSC migration and cell adhesion receptors were examined in an in vitro system. Results Mice exposed to alcohol showed decreased evidence of external callus formation, decreased callus-related osteopontin (OPN) expression levels, and decreased biomechanical stiffness. Alcohol exposure decreased rOPN-mediated MSC migration and integrin β1 receptor expression in vitro. Conclusions The effects of alcohol exposure demonstrated here on fracture callus-associated OPN expression, rOPN-mediated MSC migration in vitro, and MSC integrin β1 receptor expression in vitro have not been previously reported. Understanding the effects of alcohol exposure on the early stages of fracture repair may allow timely initiation of treatment to mitigate the long-term complications of delayed healing and/or fracture non-union.http://link.springer.com/article/10.1186/s13018-018-0800-7Bone fractureFracture non-unionAlcoholOsteopontinIntegrinMesenchymal stem cell migration |
| spellingShingle | Roman M. Natoli Henry Yu Megan Conti-Mica Meislin Pegah Abbasnia Philip Roper Aleksandra Vuchkovska Xianghui Xiao Stuart R. Stock John J. Callaci Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro Journal of Orthopaedic Surgery and Research Bone fracture Fracture non-union Alcohol Osteopontin Integrin Mesenchymal stem cell migration |
| title | Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro |
| title_full | Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro |
| title_fullStr | Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro |
| title_full_unstemmed | Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro |
| title_short | Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro |
| title_sort | alcohol exposure decreases osteopontin expression during fracture healing and osteopontin mediated mesenchymal stem cell migration in vitro |
| topic | Bone fracture Fracture non-union Alcohol Osteopontin Integrin Mesenchymal stem cell migration |
| url | http://link.springer.com/article/10.1186/s13018-018-0800-7 |
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