Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension
AIM: To observe the protective effect of human umbilical cord mesenchymal stem cells (hucMSCs) on retinal ganglion cells (RGCs) injury in mice with acute ocular hypertension (AOH). METHODS: Fifty-six adult male C57BL/6 mice were randomly divided into four groups: normal group, AOH group, hucMSCs gro...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2021-02-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2021/2/20210203.pdf |
| _version_ | 1818339320314986496 |
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| author | Rui Liu Qi Shi Hong Yang Xiao-Yuan Sha Guo-Cheng Yu Lian Liu Jing-Xiang Zhong |
| author_facet | Rui Liu Qi Shi Hong Yang Xiao-Yuan Sha Guo-Cheng Yu Lian Liu Jing-Xiang Zhong |
| author_sort | Rui Liu |
| collection | DOAJ |
| description | AIM: To observe the protective effect of human umbilical cord mesenchymal stem cells (hucMSCs) on retinal ganglion cells (RGCs) injury in mice with acute ocular hypertension (AOH). METHODS: Fifty-six adult male C57BL/6 mice were randomly divided into four groups: normal group, AOH group, hucMSCs group, normal saline (NS) group. Left eye of mice was induced by 90 mm Hg intraocular pressure for 1h to establish AOH model. hucMSCs 1×105/µL, 1 µL or NS 1 µL was injected into the vitreous body the next day. CM-Dil fluorescent dye was used to label the 3rd generation of hucMSCs, for tracing the cells in the vitreous cavity of mice. Seven days after the model established, hematoxylin-eosin (HE) staining was used to observe the thickness of the inner retina layer in four groups. Numbers and loss rate of RGCs were evaluated by counting Brn-3a positive cells stained by immunofluorescencein. RESULTS: On the 7th day after AOH established, labeled hucMSCs were found in the vitreous cavity. HE staining showed that the thickness of retinal inner layer in AOH group was significantly lower than that in normal group and hucMSCs group (P<0.05), same as that in NS group (P>0.05). Compared with AOH group, the RGCs in normal group was significantly higher; RGCs number increased in hucMSCs group and the loss rate was lower (P<0.05). Injection of NS had no protective effect on RGCs. CONCLUSION: In AOH mouse model, vitreous injection of hucMSCs have shown a protection for RGCs. |
| first_indexed | 2024-12-13T15:25:08Z |
| format | Article |
| id | doaj.art-75d9fe4174b0428a81881d5fbcc3c548 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-13T15:25:08Z |
| publishDate | 2021-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-75d9fe4174b0428a81881d5fbcc3c5482022-12-21T23:40:23ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982021-02-0114219419910.18240/ijo.2021.02.0320210203Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertensionRui Liu0Qi Shi1Hong Yang2Xiao-Yuan Sha3Guo-Cheng Yu4Lian Liu5Jing-Xiang Zhong6Jing-Xiang Zhong and Lian Liu. Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 613 Huangpu Road, Guangzhou 510630, Guangdong Province, China. zjx85221206@126.com; liulianbb@163.comDepartment of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, ChinaAIM: To observe the protective effect of human umbilical cord mesenchymal stem cells (hucMSCs) on retinal ganglion cells (RGCs) injury in mice with acute ocular hypertension (AOH). METHODS: Fifty-six adult male C57BL/6 mice were randomly divided into four groups: normal group, AOH group, hucMSCs group, normal saline (NS) group. Left eye of mice was induced by 90 mm Hg intraocular pressure for 1h to establish AOH model. hucMSCs 1×105/µL, 1 µL or NS 1 µL was injected into the vitreous body the next day. CM-Dil fluorescent dye was used to label the 3rd generation of hucMSCs, for tracing the cells in the vitreous cavity of mice. Seven days after the model established, hematoxylin-eosin (HE) staining was used to observe the thickness of the inner retina layer in four groups. Numbers and loss rate of RGCs were evaluated by counting Brn-3a positive cells stained by immunofluorescencein. RESULTS: On the 7th day after AOH established, labeled hucMSCs were found in the vitreous cavity. HE staining showed that the thickness of retinal inner layer in AOH group was significantly lower than that in normal group and hucMSCs group (P<0.05), same as that in NS group (P>0.05). Compared with AOH group, the RGCs in normal group was significantly higher; RGCs number increased in hucMSCs group and the loss rate was lower (P<0.05). Injection of NS had no protective effect on RGCs. CONCLUSION: In AOH mouse model, vitreous injection of hucMSCs have shown a protection for RGCs.http://ies.ijo.cn/en_publish/2021/2/20210203.pdfhuman umbilical cord mesenchymal stem cellsglaucomaacute ocular hypertensionretinal ganglion cells |
| spellingShingle | Rui Liu Qi Shi Hong Yang Xiao-Yuan Sha Guo-Cheng Yu Lian Liu Jing-Xiang Zhong Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension International Journal of Ophthalmology human umbilical cord mesenchymal stem cells glaucoma acute ocular hypertension retinal ganglion cells |
| title | Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension |
| title_full | Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension |
| title_fullStr | Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension |
| title_full_unstemmed | Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension |
| title_short | Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension |
| title_sort | protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension |
| topic | human umbilical cord mesenchymal stem cells glaucoma acute ocular hypertension retinal ganglion cells |
| url | http://ies.ijo.cn/en_publish/2021/2/20210203.pdf |
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