<i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats
Hypertension is accompanied by dysbiosis and a decrease in the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. However, there is no report to examine the role of <i>C. butyricum</i> in blood pressure regulation. We hypothesized that a decrease in the relative abun...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/5/4955 |
| Summary: | Hypertension is accompanied by dysbiosis and a decrease in the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. However, there is no report to examine the role of <i>C. butyricum</i> in blood pressure regulation. We hypothesized that a decrease in the relative abundance of SCFA-producing bacteria in the gut was the cause of spontaneously hypertensive rats (SHR)-induced hypertension. <i>C. butyricum</i> and captopril were used to treat adult SHR for six weeks. <i>C. butyricum</i> modulated SHR-induced dysbiosis and significantly reduced systolic blood pressure (SBP) in SHR (<i>p</i> < 0.01). A 16S rRNA analysis determined changes in the relative abundance of the mainly SCFA-producing bacteria <i>Akkermansia muciniphila</i>, <i>Lactobacillus amylovorus</i>, and <i>Agthobacter rectalis</i>, which increased significantly. Total SCFAs, and particularly butyrate concentrations, in the SHR cecum and plasma were reduced (<i>p</i> < 0.05), while <i>C. butyricum</i> prevented this effect. Likewise, we supplemented SHR with butyrate for six weeks. We analyzed the flora composition, cecum SCFA concentration, and inflammatory response. The results showed that butyrate prevented SHR-induced hypertension and inflammation, and the decline of cecum SCFA concentrations (<i>p</i> < 0.05). This research revealed that increasing cecum butyrate concentrations by probiotics, or direct butyrate supplementation, prevented the adverse effects of SHR on intestinal flora, vascular, and blood pressure. |
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| ISSN: | 1661-6596 1422-0067 |