<i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats
Hypertension is accompanied by dysbiosis and a decrease in the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. However, there is no report to examine the role of <i>C. butyricum</i> in blood pressure regulation. We hypothesized that a decrease in the relative abun...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| author | Xianshu Luo Zhuoyu Han Qing Kong Yuming Wang Haijin Mou Xuefeng Duan |
| author_facet | Xianshu Luo Zhuoyu Han Qing Kong Yuming Wang Haijin Mou Xuefeng Duan |
| author_sort | Xianshu Luo |
| collection | DOAJ |
| description | Hypertension is accompanied by dysbiosis and a decrease in the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. However, there is no report to examine the role of <i>C. butyricum</i> in blood pressure regulation. We hypothesized that a decrease in the relative abundance of SCFA-producing bacteria in the gut was the cause of spontaneously hypertensive rats (SHR)-induced hypertension. <i>C. butyricum</i> and captopril were used to treat adult SHR for six weeks. <i>C. butyricum</i> modulated SHR-induced dysbiosis and significantly reduced systolic blood pressure (SBP) in SHR (<i>p</i> < 0.01). A 16S rRNA analysis determined changes in the relative abundance of the mainly SCFA-producing bacteria <i>Akkermansia muciniphila</i>, <i>Lactobacillus amylovorus</i>, and <i>Agthobacter rectalis</i>, which increased significantly. Total SCFAs, and particularly butyrate concentrations, in the SHR cecum and plasma were reduced (<i>p</i> < 0.05), while <i>C. butyricum</i> prevented this effect. Likewise, we supplemented SHR with butyrate for six weeks. We analyzed the flora composition, cecum SCFA concentration, and inflammatory response. The results showed that butyrate prevented SHR-induced hypertension and inflammation, and the decline of cecum SCFA concentrations (<i>p</i> < 0.05). This research revealed that increasing cecum butyrate concentrations by probiotics, or direct butyrate supplementation, prevented the adverse effects of SHR on intestinal flora, vascular, and blood pressure. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-75dc8a30ec4a42eaaf5b6cd1ed10b0e32023-11-17T07:55:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245495510.3390/ijms24054955<i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive RatsXianshu Luo0Zhuoyu Han1Qing Kong2Yuming Wang3Haijin Mou4Xuefeng Duan5College of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaHypertension is accompanied by dysbiosis and a decrease in the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. However, there is no report to examine the role of <i>C. butyricum</i> in blood pressure regulation. We hypothesized that a decrease in the relative abundance of SCFA-producing bacteria in the gut was the cause of spontaneously hypertensive rats (SHR)-induced hypertension. <i>C. butyricum</i> and captopril were used to treat adult SHR for six weeks. <i>C. butyricum</i> modulated SHR-induced dysbiosis and significantly reduced systolic blood pressure (SBP) in SHR (<i>p</i> < 0.01). A 16S rRNA analysis determined changes in the relative abundance of the mainly SCFA-producing bacteria <i>Akkermansia muciniphila</i>, <i>Lactobacillus amylovorus</i>, and <i>Agthobacter rectalis</i>, which increased significantly. Total SCFAs, and particularly butyrate concentrations, in the SHR cecum and plasma were reduced (<i>p</i> < 0.05), while <i>C. butyricum</i> prevented this effect. Likewise, we supplemented SHR with butyrate for six weeks. We analyzed the flora composition, cecum SCFA concentration, and inflammatory response. The results showed that butyrate prevented SHR-induced hypertension and inflammation, and the decline of cecum SCFA concentrations (<i>p</i> < 0.05). This research revealed that increasing cecum butyrate concentrations by probiotics, or direct butyrate supplementation, prevented the adverse effects of SHR on intestinal flora, vascular, and blood pressure.https://www.mdpi.com/1422-0067/24/5/4955<i>Clostridium butyricum</i>butyratehypertensionshort chain fatty aciddysbiosis |
| spellingShingle | Xianshu Luo Zhuoyu Han Qing Kong Yuming Wang Haijin Mou Xuefeng Duan <i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats International Journal of Molecular Sciences <i>Clostridium butyricum</i> butyrate hypertension short chain fatty acid dysbiosis |
| title | <i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats |
| title_full | <i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats |
| title_fullStr | <i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats |
| title_full_unstemmed | <i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats |
| title_short | <i>Clostridium butyricum</i> Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats |
| title_sort | i clostridium butyricum i prevents dysbiosis and the rise in blood pressure in spontaneously hypertensive rats |
| topic | <i>Clostridium butyricum</i> butyrate hypertension short chain fatty acid dysbiosis |
| url | https://www.mdpi.com/1422-0067/24/5/4955 |
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