PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES
Bacteria of the genus Komagataeibacter are described to be the most noteworthy for having several of its species being efficient and strong cellulose producers. The 16S ribosomal RNA (rRNA) gene analysis is often used for the identification and taxonomic classification of these bacteria species. In...
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| Format: | Article |
| Language: | English |
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Universiti Teknologi MARA, Negeri Sembilan
2021-04-01
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| Series: | Journal of Academia |
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| Online Access: | https://myjms.mohe.gov.my/index.php/joa/article/view/11095 |
| _version_ | 1827278522600652800 |
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| author | Nur Aisyah Atikah Alizan Sarah S. Zakaria |
| author_facet | Nur Aisyah Atikah Alizan Sarah S. Zakaria |
| author_sort | Nur Aisyah Atikah Alizan |
| collection | DOAJ |
| description | Bacteria of the genus Komagataeibacter are described to be the most noteworthy for having several of its species being efficient and strong cellulose producers. The 16S ribosomal RNA (rRNA) gene analysis is often used for the identification and taxonomic classification of these bacteria species. In order to observe the phylogenetic relationship among Komagataeibacter sp., twelve sequences of the 16S rRNA gene with three sequences each for species namely Komagataeibacter europaeus, Komagataeibacter hansenii, Komagataeibacter intermedius and Komagataeibacter xylinus were retrieved from NCBI GenBank database. The sequences were aligned and analysed using PAUP, OrthoANI and BLAST, followed by the phylogenetic tree construction using a Maximum Likelihood method. The parsimony character diagnostic analysis showed very few numbers of parsimony-informative characters present in the aligned sequences which is only 1.5% of the total characters. The inferred phylogenetic relationships demonstrated the unexpected positioning of K. xylinus (GQ240638: Gluconacetobacter xylinus strain) and K. xylinus (KC11853: G. xylinus strain) into the clades of K. europaeus and K. hansenii respectively. The also very low bootstrap values of the branch points linking the K. europaeus species indicated low support for the produced topologies. The findings of this study indicate that more phylogenies information can be attained by increasing the taxon sampling. In addition, more robust molecular data are needed to infer the phylogenetic relationships between the Komagataeibacter species more accurately. |
| first_indexed | 2024-04-13T21:25:04Z |
| format | Article |
| id | doaj.art-75e2fff76c2b4a898a84aa1116f852c0 |
| institution | Directory Open Access Journal |
| issn | 2289-6368 |
| language | English |
| last_indexed | 2024-04-24T08:01:37Z |
| publishDate | 2021-04-01 |
| publisher | Universiti Teknologi MARA, Negeri Sembilan |
| record_format | Article |
| series | Journal of Academia |
| spelling | doaj.art-75e2fff76c2b4a898a84aa1116f852c02024-04-17T16:36:03ZengUniversiti Teknologi MARA, Negeri SembilanJournal of Academia2289-63682021-04-019197105PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCESNur Aisyah Atikah Alizan0Sarah S. Zakaria1Universiti Teknologi MARAUniversiti Teknologi MARABacteria of the genus Komagataeibacter are described to be the most noteworthy for having several of its species being efficient and strong cellulose producers. The 16S ribosomal RNA (rRNA) gene analysis is often used for the identification and taxonomic classification of these bacteria species. In order to observe the phylogenetic relationship among Komagataeibacter sp., twelve sequences of the 16S rRNA gene with three sequences each for species namely Komagataeibacter europaeus, Komagataeibacter hansenii, Komagataeibacter intermedius and Komagataeibacter xylinus were retrieved from NCBI GenBank database. The sequences were aligned and analysed using PAUP, OrthoANI and BLAST, followed by the phylogenetic tree construction using a Maximum Likelihood method. The parsimony character diagnostic analysis showed very few numbers of parsimony-informative characters present in the aligned sequences which is only 1.5% of the total characters. The inferred phylogenetic relationships demonstrated the unexpected positioning of K. xylinus (GQ240638: Gluconacetobacter xylinus strain) and K. xylinus (KC11853: G. xylinus strain) into the clades of K. europaeus and K. hansenii respectively. The also very low bootstrap values of the branch points linking the K. europaeus species indicated low support for the produced topologies. The findings of this study indicate that more phylogenies information can be attained by increasing the taxon sampling. In addition, more robust molecular data are needed to infer the phylogenetic relationships between the Komagataeibacter species more accurately.https://myjms.mohe.gov.my/index.php/joa/article/view/11095phylogenetics16s rrnaacetobacterbacterial cellulosekomagataeibacter |
| spellingShingle | Nur Aisyah Atikah Alizan Sarah S. Zakaria PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES Journal of Academia phylogenetics 16s rrna acetobacter bacterial cellulose komagataeibacter |
| title | PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES |
| title_full | PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES |
| title_fullStr | PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES |
| title_full_unstemmed | PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES |
| title_short | PHYLOGENETIC ANALYSIS OF Komagataeibacter sp.: A CELLULOSE-PRODUCER BACTERIA BASED ON 16S rRNA GENE SEQUENCES |
| title_sort | phylogenetic analysis of komagataeibacter sp a cellulose producer bacteria based on 16s rrna gene sequences |
| topic | phylogenetics 16s rrna acetobacter bacterial cellulose komagataeibacter |
| url | https://myjms.mohe.gov.my/index.php/joa/article/view/11095 |
| work_keys_str_mv | AT nuraisyahatikahalizan phylogeneticanalysisofkomagataeibacterspacelluloseproducerbacteriabasedon16srrnagenesequences AT sarahszakaria phylogeneticanalysisofkomagataeibacterspacelluloseproducerbacteriabasedon16srrnagenesequences |