Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup>
Astragali Radix total flavonoids (ARTF) is one of the main bioactive components of Astragali Radix (AR), and has many pharmacological effects. However, its metabolism and effective forms remains unclear. The HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> technique was used to screen and tentatively...
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2020-11-01
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author | Li-Jia Liu Hong-Fu Li Feng Xu Hong-Yan Wang Yi-Fan Zhang Guang-Xue Liu Ming-Ying Shang Xuan Wang Shao-Qing Cai |
author_facet | Li-Jia Liu Hong-Fu Li Feng Xu Hong-Yan Wang Yi-Fan Zhang Guang-Xue Liu Ming-Ying Shang Xuan Wang Shao-Qing Cai |
author_sort | Li-Jia Liu |
collection | DOAJ |
description | Astragali Radix total flavonoids (ARTF) is one of the main bioactive components of Astragali Radix (AR), and has many pharmacological effects. However, its metabolism and effective forms remains unclear. The HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> technique was used to screen and tentatively identify the in vivo original constituents and metabolites of ARTF and to clarify their distribution in rats after oral administration. In addition, modern chromatographic methods were used to isolate the main metabolites from rat urine and NMR spectroscopy was used to elucidate their structures. As a result, 170 compounds (23 original constituents and 147 metabolites) were tentatively identified as forms existing in vivo, 13 of which have the same pharmacological effect with ARTF. Among 170 compounds, three were newly detected original constituents in vivo and 89 were new metabolites of ARTF, from which 12 metabolites were regarded as new compounds. Nineteen original constituents and 65 metabolites were detected in 10 organs. Four metabolites were isolated and identified from rat urine, including a new compound (calycoisn-3’-<i>O</i>-glucuronide methyl ester), a firstly-isolated metabolite (astraisoflavan-7-<i>O</i>-glucoside-2’-<i>O</i>-glucuronide), and two known metabolites (daidzein-7-<i>O</i>-sulfate and calycosin-3’-<i>O</i>-glucuronide). The original constituents and metabolites existing in vivo may be material basis for ARTF efficacy, and these findings are helpful for further clarifying the effective forms of ARTF. |
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language | English |
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spelling | doaj.art-75e56c4f0d1d4c2caade6dbf055867732023-11-20T22:31:27ZengMDPI AGMolecules1420-30492020-11-012523556010.3390/molecules25235560Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup>Li-Jia Liu0Hong-Fu Li1Feng Xu2Hong-Yan Wang3Yi-Fan Zhang4Guang-Xue Liu5Ming-Ying Shang6Xuan Wang7Shao-Qing Cai8State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, ChinaAstragali Radix total flavonoids (ARTF) is one of the main bioactive components of Astragali Radix (AR), and has many pharmacological effects. However, its metabolism and effective forms remains unclear. The HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> technique was used to screen and tentatively identify the in vivo original constituents and metabolites of ARTF and to clarify their distribution in rats after oral administration. In addition, modern chromatographic methods were used to isolate the main metabolites from rat urine and NMR spectroscopy was used to elucidate their structures. As a result, 170 compounds (23 original constituents and 147 metabolites) were tentatively identified as forms existing in vivo, 13 of which have the same pharmacological effect with ARTF. Among 170 compounds, three were newly detected original constituents in vivo and 89 were new metabolites of ARTF, from which 12 metabolites were regarded as new compounds. Nineteen original constituents and 65 metabolites were detected in 10 organs. Four metabolites were isolated and identified from rat urine, including a new compound (calycoisn-3’-<i>O</i>-glucuronide methyl ester), a firstly-isolated metabolite (astraisoflavan-7-<i>O</i>-glucoside-2’-<i>O</i>-glucuronide), and two known metabolites (daidzein-7-<i>O</i>-sulfate and calycosin-3’-<i>O</i>-glucuronide). The original constituents and metabolites existing in vivo may be material basis for ARTF efficacy, and these findings are helpful for further clarifying the effective forms of ARTF.https://www.mdpi.com/1420-3049/25/23/5560Astragali RadixflavonoidsmetabolismLC-MSeffective forms |
spellingShingle | Li-Jia Liu Hong-Fu Li Feng Xu Hong-Yan Wang Yi-Fan Zhang Guang-Xue Liu Ming-Ying Shang Xuan Wang Shao-Qing Cai Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> Molecules Astragali Radix flavonoids metabolism LC-MS effective forms |
title | Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> |
title_full | Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> |
title_fullStr | Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> |
title_full_unstemmed | Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> |
title_short | Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MS<sup>n</sup> |
title_sort | exploring the in vivo existence forms 23 original constituents and 147 metabolites of astragali radix total flavonoids and their distributions in rats using hplc dad esi it tof ms sup n sup |
topic | Astragali Radix flavonoids metabolism LC-MS effective forms |
url | https://www.mdpi.com/1420-3049/25/23/5560 |
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