Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance
AIM: To evaluate the relationship between gene polymorphism (BclI, ER22/23EK, N363S) and the occurrence, progression and sensitivity to glucocorticoid of lacrimal gland benign lymphoepithelial lesion (LGBLEL). METHODS: Clinical peripheral blood samples of 52 LGBLEL patients and 10 normal volunteers...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2023-11-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/en_publish/2023/11/20231102.pdf |
| _version_ | 1797650554230407168 |
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| author | Xu-Juan Zhang Peng-Xiang Zhao Ming-Shen Ma Hao Wu Rui Liu Hui Wang Meng-Yu Liu Fei Xie Xue-Mei Ma |
| author_facet | Xu-Juan Zhang Peng-Xiang Zhao Ming-Shen Ma Hao Wu Rui Liu Hui Wang Meng-Yu Liu Fei Xie Xue-Mei Ma |
| author_sort | Xu-Juan Zhang |
| collection | DOAJ |
| description | AIM: To evaluate the relationship between gene polymorphism (BclI, ER22/23EK, N363S) and the occurrence, progression and sensitivity to glucocorticoid of lacrimal gland benign lymphoepithelial lesion (LGBLEL). METHODS: Clinical peripheral blood samples of 52 LGBLEL patients and 10 normal volunteers were collected for DNA extraction and polymerase chain reaction sequencing to analyze single nucleotide polymorphism (SNP) genotypes. The lacrimal tissues of LGBLEL were surgically removed and made into paraffin sections for subsequent hematoxylin-eosin (HE) and Masson staining analysis. The duration of disease and hormone use of LGBLEL patients from diagnosis to surgery were also analyzed. The Meta-analysis follows PRISMA guidelines to conducted a systematic review of human studies investigating the relationship between the NR3C1 BclI polymorphism and glucocorticoids (GCs) sensitivity. RESULTS: There was no association between ER22/23EK or N363S and the occurrence of LGBLEL or GCs sensitivity (P>0.05); BclI GC genotype was closely related to GCs resistance (P=0.03) as is the minor allele C (P=0.0017). The HE staining and Masson staining showed that the GC genotype of BclI remarkably slowed down the disease progression and reduced fibrosis (P<0.05), especially for GCs-dependent patients (P<0.0001). Meta-analysis showed that BclI was not significantly associated with GCs responsiveness. CONCLUSION: The LGBLEL patients who carry the NR3C1 BclI allele C may be more sensitive to GCs and associated with lower fibrosis and slower disease progression. The results may guide the clinical treatment strategy for the LGBLEL patients. |
| first_indexed | 2024-03-11T16:02:02Z |
| format | Article |
| id | doaj.art-75e6a73c87684ea5a20a3c9fa0c7b4c7 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-03-11T16:02:02Z |
| publishDate | 2023-11-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-75e6a73c87684ea5a20a3c9fa0c7b4c72023-10-25T06:50:11ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982023-11-0116111734174510.18240/ijo.2023.11.0220231102Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistanceXu-Juan Zhang0Peng-Xiang Zhao1Ming-Shen Ma2Hao Wu3Rui Liu4Hui Wang5Meng-Yu Liu6Fei Xie7Xue-Mei Ma8Peng-Xiang Zhao and Xue-Mei Ma. Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China. zpx@bjut.edu.cn; xmma@bjut.edu.cnFaculty of Environment and Life, Beijing University of Technology, Beijing 100124, China; Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, ChinaBeijing Tongren Hospital, Capital Medical University, Beijing 100730, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing 100124, China; Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, ChinaBeijing Tongren Hospital, Capital Medical University, Beijing 100730, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing 100124, China; Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, China; State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Department of General Dentistry and Emergency, School of Stomatology, Air Force Medical UniversityFaculty of Environment and Life, Beijing University of Technology, Beijing 100124, China; Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing 100124, China; Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing 100124, China; Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, ChinaAIM: To evaluate the relationship between gene polymorphism (BclI, ER22/23EK, N363S) and the occurrence, progression and sensitivity to glucocorticoid of lacrimal gland benign lymphoepithelial lesion (LGBLEL). METHODS: Clinical peripheral blood samples of 52 LGBLEL patients and 10 normal volunteers were collected for DNA extraction and polymerase chain reaction sequencing to analyze single nucleotide polymorphism (SNP) genotypes. The lacrimal tissues of LGBLEL were surgically removed and made into paraffin sections for subsequent hematoxylin-eosin (HE) and Masson staining analysis. The duration of disease and hormone use of LGBLEL patients from diagnosis to surgery were also analyzed. The Meta-analysis follows PRISMA guidelines to conducted a systematic review of human studies investigating the relationship between the NR3C1 BclI polymorphism and glucocorticoids (GCs) sensitivity. RESULTS: There was no association between ER22/23EK or N363S and the occurrence of LGBLEL or GCs sensitivity (P>0.05); BclI GC genotype was closely related to GCs resistance (P=0.03) as is the minor allele C (P=0.0017). The HE staining and Masson staining showed that the GC genotype of BclI remarkably slowed down the disease progression and reduced fibrosis (P<0.05), especially for GCs-dependent patients (P<0.0001). Meta-analysis showed that BclI was not significantly associated with GCs responsiveness. CONCLUSION: The LGBLEL patients who carry the NR3C1 BclI allele C may be more sensitive to GCs and associated with lower fibrosis and slower disease progression. The results may guide the clinical treatment strategy for the LGBLEL patients.http://ies.ijo.cn/en_publish/2023/11/20231102.pdflacrimal gland benign lymphoepithelial lesionbclisingle nucleotide polymorphismsglucocorticoids resistancefibrosis |
| spellingShingle | Xu-Juan Zhang Peng-Xiang Zhao Ming-Shen Ma Hao Wu Rui Liu Hui Wang Meng-Yu Liu Fei Xie Xue-Mei Ma Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance International Journal of Ophthalmology lacrimal gland benign lymphoepithelial lesion bcli single nucleotide polymorphisms glucocorticoids resistance fibrosis |
| title | Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance |
| title_full | Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance |
| title_fullStr | Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance |
| title_full_unstemmed | Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance |
| title_short | Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance |
| title_sort | association analysis of bcli with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance |
| topic | lacrimal gland benign lymphoepithelial lesion bcli single nucleotide polymorphisms glucocorticoids resistance fibrosis |
| url | http://ies.ijo.cn/en_publish/2023/11/20231102.pdf |
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