Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy
Abstract Background Lipocalin-2 (LCN2) is a novel adipokine with potential roles in obesity, insulin resistance, and inflammation. This study aims to assess the concentrations of LCN2 and vascular endothelial growth factor (VEGF) expressed in the vitreous humors of patients with proliferative diabet...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12886-020-01462-5 |
| _version_ | 1819105904748920832 |
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| author | Hui Wang Hui Lou Yongrong Li Fengtao Ji Wei Chen Qianyi Lu Guoxu Xu |
| author_facet | Hui Wang Hui Lou Yongrong Li Fengtao Ji Wei Chen Qianyi Lu Guoxu Xu |
| author_sort | Hui Wang |
| collection | DOAJ |
| description | Abstract Background Lipocalin-2 (LCN2) is a novel adipokine with potential roles in obesity, insulin resistance, and inflammation. This study aims to assess the concentrations of LCN2 and vascular endothelial growth factor (VEGF) expressed in the vitreous humors of patients with proliferative diabetic retinopathy (PDR). Methods The concentrations of LCN2 and VEGF were measured from the vitreous of 67 patients undergoing vitrectomy (20 controls and 47 PDR) via enzyme-linked immunosorbent assay (ELISA). Patients with non-ocular pathology that could elevate the LCN2 level in the vitreous were excluded. PDR activity and a history of panretinal photocoagulation were used for further grouping analysis. Results The vitreous concentration of LCN2 was statistically significantly higher in the PDR group compared to the control group (63,522 (30,009) pg/ml versus 1663 (1191) pg/ml, respectively; P < 0.001). VEGF level was also significantly higher in the PDR group than in the control group (1038 (1326) pg/ml versus 9 pg/ml, respectively; P < 0.001). The mean vitreous LCN2 and VEGF levels in active PDR patients were significantly higher than that of the inactive PDR patients. The mean LCN2 concentration in vitreous humor was significantly lower in the 28 PDR patients with a history of complete PRP (37,304 (16,651) pg/mL) in comparison with 19 PDR patients without preperformed panretinal photocoagulation or with preperformed incomplete panretinal photocoagulation (79,796 (24,391) pg/mL). A significant correlation between the vitreous LCN2 level and VEGF level was found in patients with PDR (R = 0.34; P = 0.019). Conclusions This report shows a significant increase of LCN2 in the vitreous fluid of patients with PDR and present a significant correlation between LCN2 and VEGF, suggesting LCN2 might be involved in the pathogenesis of PDR. |
| first_indexed | 2024-12-22T02:29:40Z |
| format | Article |
| id | doaj.art-75e757aa8eed420481bfddfbbc03521c |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-22T02:29:40Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-75e757aa8eed420481bfddfbbc03521c2022-12-21T18:41:55ZengBMCBMC Ophthalmology1471-24152020-06-012011710.1186/s12886-020-01462-5Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathyHui Wang0Hui Lou1Yongrong Li2Fengtao Ji3Wei Chen4Qianyi Lu5Guoxu Xu6Department of Ophthalmology, The Second Affiliated Hospital of Soochow UniversityDepartment of Ophthalmology, The Second Affiliated Hospital of Soochow UniversityDepartment of Ophthalmology, The Second People’s Hospital of HefeiDepartment of Ophthalmology, The Second People’s Hospital of HefeiDepartment of Ophthalmology, Affiliated Hospital of Nantong UniversityDepartment of Ophthalmology, The First Affiliated Hospital of Soochow UniversityDepartment of Ophthalmology, The Second Affiliated Hospital of Soochow UniversityAbstract Background Lipocalin-2 (LCN2) is a novel adipokine with potential roles in obesity, insulin resistance, and inflammation. This study aims to assess the concentrations of LCN2 and vascular endothelial growth factor (VEGF) expressed in the vitreous humors of patients with proliferative diabetic retinopathy (PDR). Methods The concentrations of LCN2 and VEGF were measured from the vitreous of 67 patients undergoing vitrectomy (20 controls and 47 PDR) via enzyme-linked immunosorbent assay (ELISA). Patients with non-ocular pathology that could elevate the LCN2 level in the vitreous were excluded. PDR activity and a history of panretinal photocoagulation were used for further grouping analysis. Results The vitreous concentration of LCN2 was statistically significantly higher in the PDR group compared to the control group (63,522 (30,009) pg/ml versus 1663 (1191) pg/ml, respectively; P < 0.001). VEGF level was also significantly higher in the PDR group than in the control group (1038 (1326) pg/ml versus 9 pg/ml, respectively; P < 0.001). The mean vitreous LCN2 and VEGF levels in active PDR patients were significantly higher than that of the inactive PDR patients. The mean LCN2 concentration in vitreous humor was significantly lower in the 28 PDR patients with a history of complete PRP (37,304 (16,651) pg/mL) in comparison with 19 PDR patients without preperformed panretinal photocoagulation or with preperformed incomplete panretinal photocoagulation (79,796 (24,391) pg/mL). A significant correlation between the vitreous LCN2 level and VEGF level was found in patients with PDR (R = 0.34; P = 0.019). Conclusions This report shows a significant increase of LCN2 in the vitreous fluid of patients with PDR and present a significant correlation between LCN2 and VEGF, suggesting LCN2 might be involved in the pathogenesis of PDR.http://link.springer.com/article/10.1186/s12886-020-01462-5Diabetic retinopathyVascular endothelial growth factorLipocalin-2 |
| spellingShingle | Hui Wang Hui Lou Yongrong Li Fengtao Ji Wei Chen Qianyi Lu Guoxu Xu Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy BMC Ophthalmology Diabetic retinopathy Vascular endothelial growth factor Lipocalin-2 |
| title | Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy |
| title_full | Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy |
| title_fullStr | Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy |
| title_full_unstemmed | Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy |
| title_short | Elevated vitreous Lipocalin-2 levels of patients with proliferative diabetic retinopathy |
| title_sort | elevated vitreous lipocalin 2 levels of patients with proliferative diabetic retinopathy |
| topic | Diabetic retinopathy Vascular endothelial growth factor Lipocalin-2 |
| url | http://link.springer.com/article/10.1186/s12886-020-01462-5 |
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