The Inhibition Effect of Paliurus Spina-Christi Methanolic Extract on Trichophyton Mentagrophytes Growth and Tri M4 Gene
Back ground: Paliurus spina-christi methanolic extract is rich of phenolic compounds as well as other biological active compounds (sugars ,esters, fatty acids and alkaloids) . Aim:To asses that Paliurus spina-christi a good anti dermatophytic activity (Trichophyton mentagrophytes) . Mate...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of medicine/ University of Diyala
2019-11-01
|
| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | http://djm.uodiyala.edu.iq/index.php/djm/article/view/416 |
| Summary: | Back ground: Paliurus spina-christi methanolic extract is rich of phenolic compounds as well as other biological active compounds (sugars ,esters, fatty acids and alkaloids) .
Aim:To asses that Paliurus spina-christi a good anti dermatophytic activity (Trichophyton mentagrophytes) .
Materials and methods: five different concentrations of methanolic extract of P. spina-christi against T. mentagrophytes followed by the RNA extraction of Ttichophyton mentagrophytes grown in media containing P. spina-christi methanolic extract followed by complementary DNA synthesis to examine the expression of Tri m4 gene .
Results: Statistical analysis revealed that at concentrations(25 and 50)µg/ml the differences between means of the growth diameters are non-significant (P≥ 0.05) as compared with the control while at concentrations (75,100,125)µg/ml the effect of methanolic extract on diameter growth was statistically significant (P≤0.05). Maximum growth inhibition was at 125µg/ml while minimum inhibition was at concentration 25 µg/ml .The percentage of inhibition was statistically significant especially at concentration 125 µg/ml it recorded 82%. The expression of Tri m4 gene was identified in presence of extract .
Conclusion :Methanolic extract of P. spina-christi didn’t affect the expression of Tri m4 gene.
|
|---|---|
| ISSN: | 2219-9764 2617-8982 |