A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity
While electrospun chitosan membranes modified to retain nanofibrous morphology have shown promise for use in guided bone regeneration applications in in vitro and in vivo studies, their mechanical tear strengths are lower than commercial collagen membranes. Elastin, a natural component of the extrac...
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MDPI AG
2021-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/3/169 |
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| author | Hengjie Su Tomoko Fujiwara Joel D. Bumgardner |
| author_facet | Hengjie Su Tomoko Fujiwara Joel D. Bumgardner |
| author_sort | Hengjie Su |
| collection | DOAJ |
| description | While electrospun chitosan membranes modified to retain nanofibrous morphology have shown promise for use in guided bone regeneration applications in in vitro and in vivo studies, their mechanical tear strengths are lower than commercial collagen membranes. Elastin, a natural component of the extracellular matrix, is a protein with extensive elastic property. This work examined the incorporation of elastin into electrospun chitosan membranes to improve their mechanical tear strengths and to further mimic the native extracellular composition for guided bone regeneration (GBR) applications. In this work, hydrolyzed elastin (ES12, Elastin Products Company, USA) was added to a chitosan spinning solution from 0 to 4 wt% of chitosan. The chitosan–elastin (CE) membranes were examined for fiber morphology using SEM, hydrophobicity using water contact angle measurements, the mechanical tear strength under simulated surgical tacking, and compositions using Fourier-transform infrared spectroscopy (FTIR) and post-spinning protein extraction. In vitro experiments were conducted to evaluate the degradation in a lysozyme solution based on the mass loss and growth of fibroblastic cells. Chitosan membranes with elastin showed significantly thicker fiber diameters, lower water contact angles, up to 33% faster degradation rates, and up to seven times higher mechanical strengths than the chitosan membrane. The FTIR spectra showed stronger amide peaks at 1535 cm<sup>−1</sup> and 1655 cm<sup>−1</sup> in membranes with higher concentrated elastin, indicating the incorporation of elastin into electrospun fibers. The bicinchoninic acid (BCA) assay demonstrated an increase in protein concentration in proportion to the amount of elastin added to the CE membranes. In addition, all the CE membranes showed in vitro biocompatibility with the fibroblasts. |
| first_indexed | 2024-03-10T13:02:07Z |
| format | Article |
| id | doaj.art-75ed9e39dec34aa4862786a43ebdfe4a |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T13:02:07Z |
| publishDate | 2021-03-01 |
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| series | Marine Drugs |
| spelling | doaj.art-75ed9e39dec34aa4862786a43ebdfe4a2023-11-21T11:27:34ZengMDPI AGMarine Drugs1660-33972021-03-0119316910.3390/md19030169A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and BioactivityHengjie Su0Tomoko Fujiwara1Joel D. Bumgardner2Department of Biomedical Engineering, UT-UofM Joint Graduate Program in Biomedical Engineering, The University of Memphis, Engineering Technology Bldg #330, Memphis, TN 38152, USADepartment of Chemistry, The University of Memphis, Smith Hall #409, Memphis, TN 38152, USADepartment of Biomedical Engineering, UT-UofM Joint Graduate Program in Biomedical Engineering, The University of Memphis, Engineering Technology Bldg #330, Memphis, TN 38152, USAWhile electrospun chitosan membranes modified to retain nanofibrous morphology have shown promise for use in guided bone regeneration applications in in vitro and in vivo studies, their mechanical tear strengths are lower than commercial collagen membranes. Elastin, a natural component of the extracellular matrix, is a protein with extensive elastic property. This work examined the incorporation of elastin into electrospun chitosan membranes to improve their mechanical tear strengths and to further mimic the native extracellular composition for guided bone regeneration (GBR) applications. In this work, hydrolyzed elastin (ES12, Elastin Products Company, USA) was added to a chitosan spinning solution from 0 to 4 wt% of chitosan. The chitosan–elastin (CE) membranes were examined for fiber morphology using SEM, hydrophobicity using water contact angle measurements, the mechanical tear strength under simulated surgical tacking, and compositions using Fourier-transform infrared spectroscopy (FTIR) and post-spinning protein extraction. In vitro experiments were conducted to evaluate the degradation in a lysozyme solution based on the mass loss and growth of fibroblastic cells. Chitosan membranes with elastin showed significantly thicker fiber diameters, lower water contact angles, up to 33% faster degradation rates, and up to seven times higher mechanical strengths than the chitosan membrane. The FTIR spectra showed stronger amide peaks at 1535 cm<sup>−1</sup> and 1655 cm<sup>−1</sup> in membranes with higher concentrated elastin, indicating the incorporation of elastin into electrospun fibers. The bicinchoninic acid (BCA) assay demonstrated an increase in protein concentration in proportion to the amount of elastin added to the CE membranes. In addition, all the CE membranes showed in vitro biocompatibility with the fibroblasts.https://www.mdpi.com/1660-3397/19/3/169electrospinningchitosanelastinmechanical strength |
| spellingShingle | Hengjie Su Tomoko Fujiwara Joel D. Bumgardner A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity Marine Drugs electrospinning chitosan elastin mechanical strength |
| title | A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity |
| title_full | A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity |
| title_fullStr | A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity |
| title_full_unstemmed | A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity |
| title_short | A Study of Combining Elastin in the Chitosan Electrospinning to Increase the Mechanical Strength and Bioactivity |
| title_sort | study of combining elastin in the chitosan electrospinning to increase the mechanical strength and bioactivity |
| topic | electrospinning chitosan elastin mechanical strength |
| url | https://www.mdpi.com/1660-3397/19/3/169 |
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