Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles
Bacterial extracellular vesicles (EVs) perform various biological functions, including those that are critical to microbes. Determination of EVs composition allows for a deep understanding of their role in the bacterial community and communication among them. <i>Cutibacterium acnes</i>,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5797 |
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| author | Anna Chudzik Paweł Migdał Mariola Paściak |
| author_facet | Anna Chudzik Paweł Migdał Mariola Paściak |
| author_sort | Anna Chudzik |
| collection | DOAJ |
| description | Bacterial extracellular vesicles (EVs) perform various biological functions, including those that are critical to microbes. Determination of EVs composition allows for a deep understanding of their role in the bacterial community and communication among them. <i>Cutibacterium acnes</i>, formerly <i>Propionibacterium</i><i>acnes</i>, are commensal bacteria responsible for various infections, e.g., prosthesis, sarcoidosis, soft-tissue infections, and the most known but still controversial—acnes lesion. In <i>C. acnes</i>, three major phylotypes represented variable disease associations. Herein, for the first time, we present a comparative analysis of EVs obtained from three <i>C. acnes</i> phylotypes (IA1, IB, and II) to demonstrate the existence of differences in their protein and lipid composition. In the following work, the morphological analysis of EVs was performed, and the SDS-PAGE protein profile and the lipid profile were presented using the TLC and MALDI-TOF MS methods. This study allowed us to show major differences between the protein and lipid composition of <i>C. acnes</i> EVs. This is a clear indication that EVs released by different phylotypes of the one species are not identical to each other in terms of composition and should be separately analyzed each time to obtain reliable results. |
| first_indexed | 2024-03-10T03:42:33Z |
| format | Article |
| id | doaj.art-75efae4f75184f2fb8a01f5e21488397 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:42:33Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-75efae4f75184f2fb8a01f5e214883972023-11-23T11:28:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310579710.3390/ijms23105797Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular VesiclesAnna Chudzik0Paweł Migdał1Mariola Paściak2Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, PolandBacterial extracellular vesicles (EVs) perform various biological functions, including those that are critical to microbes. Determination of EVs composition allows for a deep understanding of their role in the bacterial community and communication among them. <i>Cutibacterium acnes</i>, formerly <i>Propionibacterium</i><i>acnes</i>, are commensal bacteria responsible for various infections, e.g., prosthesis, sarcoidosis, soft-tissue infections, and the most known but still controversial—acnes lesion. In <i>C. acnes</i>, three major phylotypes represented variable disease associations. Herein, for the first time, we present a comparative analysis of EVs obtained from three <i>C. acnes</i> phylotypes (IA1, IB, and II) to demonstrate the existence of differences in their protein and lipid composition. In the following work, the morphological analysis of EVs was performed, and the SDS-PAGE protein profile and the lipid profile were presented using the TLC and MALDI-TOF MS methods. This study allowed us to show major differences between the protein and lipid composition of <i>C. acnes</i> EVs. This is a clear indication that EVs released by different phylotypes of the one species are not identical to each other in terms of composition and should be separately analyzed each time to obtain reliable results.https://www.mdpi.com/1422-0067/23/10/5797extracellular vesiclesmicrovesicles<i>Cutibacterium acnes</i>lipid analysisprotein analysisSDS-PAGE |
| spellingShingle | Anna Chudzik Paweł Migdał Mariola Paściak Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles International Journal of Molecular Sciences extracellular vesicles microvesicles <i>Cutibacterium acnes</i> lipid analysis protein analysis SDS-PAGE |
| title | Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles |
| title_full | Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles |
| title_fullStr | Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles |
| title_full_unstemmed | Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles |
| title_short | Different <i>Cutibacterium acnes</i> Phylotypes Release Distinct Extracellular Vesicles |
| title_sort | different i cutibacterium acnes i phylotypes release distinct extracellular vesicles |
| topic | extracellular vesicles microvesicles <i>Cutibacterium acnes</i> lipid analysis protein analysis SDS-PAGE |
| url | https://www.mdpi.com/1422-0067/23/10/5797 |
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