| Summary: | The objectives of this work were to elucidate alterations in the compaction of sperm chromatin during storage in the sperm storage tubules of fowl hens, to observe the storage time, and to estimate the variation in the amount of stored spermatozoa in the cranial, medium and caudal sections of the uterovaginal junction over a period of 23 days. For the analysis of the spermatozoa during storage in the sperm storage tubules of hens, 48 thirty-six-week-old meat chickens of the Cobb Avian 48 strain were used. Samples from the uterovaginal junction of the hens were collected for 23 days after the mating with the roosters, and six hens were euthanized every four days for the production of histological microscope slides. Spermatozoa were found throughout the area of the sperm storage tubules. Most of them were gathered in the medium section of the uterovaginal junction. There was no meaningful statistical difference in the amount of sperm in the sperm storage tubules over the days of the experiment. A greater amount of sperm cells were observed up to the 23rd day of assessment. Therefore, the storage of spermatozoa in hens lasts at least 23 days.
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