Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae
Abstract Background Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-ba...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | https://doi.org/10.1186/s13068-021-01878-1 |
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| author | Hsiang-Yen Su Hua-Ying Li Cai-Yun Xie Qiang Fei Ke-Ke Cheng |
| author_facet | Hsiang-Yen Su Hua-Ying Li Cai-Yun Xie Qiang Fei Ke-Ke Cheng |
| author_sort | Hsiang-Yen Su |
| collection | DOAJ |
| description | Abstract Background Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-based production processes depending on fossil resources. Therefore, we engineered an Enterobacter cloacae budC and ldhA double-deletion strain (namely, EC∆budC∆ldhA) to redirect carbon fluxes and optimized the culture conditions to co-produce succinic acid and acetoin. Results In this work, E. cloacae was metabolically engineered to enhance its combined succinic acid and acetoin production during fermentation. Strain EC∆budC∆ldhA was constructed by deleting 2,3-butanediol dehydrogenase (budC), which is involved in 2,3-butanediol production, and lactate dehydrogenase (ldhA), which is involved in lactic acid production, from the E. cloacae genome. After redirecting and fine-tuning the E. cloacae metabolic flux, succinic acid and acetoin production was enhanced, and the combined production titers of acetoin and succinic acid from glucose were 17.75 and 2.75 g L−1, respectively. Moreover, to further improve acetoin and succinic acid production, glucose and NaHCO3 modes and times of feeding were optimized during fermentation of the EC∆budC∆ldhA strain. The maximum titers of acetoin and succinic acid were 39.5 and 20.3 g L−1 at 72 h, respectively. Conclusions The engineered strain EC∆budC∆ldhA is useful for the co-production of acetoin and succinic acid and for reducing microbial fermentation costs by combining processes into a single step. |
| first_indexed | 2024-04-13T22:16:30Z |
| format | Article |
| id | doaj.art-75f09b59470e489dae6dc9157dc8b55e |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:16:30Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-75f09b59470e489dae6dc9157dc8b55e2022-12-22T02:27:29ZengBMCBiotechnology for Biofuels1754-68342021-01-0114111110.1186/s13068-021-01878-1Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacaeHsiang-Yen Su0Hua-Ying Li1Cai-Yun Xie2Qiang Fei3Ke-Ke Cheng4Engineering Research Center of Health Food Design & Nutrition Regulation, School of Chemical Engineering and Energy Technology, Dongguan University of TechnologyChina-Latin America Joint Laboratory for Clean Energy and Climate Change, School of Chemical Engineering and Energy Technology, Dongguan University of TechnologyChina-Latin America Joint Laboratory for Clean Energy and Climate Change, School of Chemical Engineering and Energy Technology, Dongguan University of TechnologySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityEngineering Research Center of Health Food Design & Nutrition Regulation, School of Chemical Engineering and Energy Technology, Dongguan University of TechnologyAbstract Background Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-based production processes depending on fossil resources. Therefore, we engineered an Enterobacter cloacae budC and ldhA double-deletion strain (namely, EC∆budC∆ldhA) to redirect carbon fluxes and optimized the culture conditions to co-produce succinic acid and acetoin. Results In this work, E. cloacae was metabolically engineered to enhance its combined succinic acid and acetoin production during fermentation. Strain EC∆budC∆ldhA was constructed by deleting 2,3-butanediol dehydrogenase (budC), which is involved in 2,3-butanediol production, and lactate dehydrogenase (ldhA), which is involved in lactic acid production, from the E. cloacae genome. After redirecting and fine-tuning the E. cloacae metabolic flux, succinic acid and acetoin production was enhanced, and the combined production titers of acetoin and succinic acid from glucose were 17.75 and 2.75 g L−1, respectively. Moreover, to further improve acetoin and succinic acid production, glucose and NaHCO3 modes and times of feeding were optimized during fermentation of the EC∆budC∆ldhA strain. The maximum titers of acetoin and succinic acid were 39.5 and 20.3 g L−1 at 72 h, respectively. Conclusions The engineered strain EC∆budC∆ldhA is useful for the co-production of acetoin and succinic acid and for reducing microbial fermentation costs by combining processes into a single step.https://doi.org/10.1186/s13068-021-01878-1Enterobacter cloacaeMetabolic engineeringCo-productionAcetoinSuccinic acid |
| spellingShingle | Hsiang-Yen Su Hua-Ying Li Cai-Yun Xie Qiang Fei Ke-Ke Cheng Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae Biotechnology for Biofuels Enterobacter cloacae Metabolic engineering Co-production Acetoin Succinic acid |
| title | Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae |
| title_full | Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae |
| title_fullStr | Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae |
| title_full_unstemmed | Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae |
| title_short | Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae |
| title_sort | co production of acetoin and succinic acid by metabolically engineered enterobacter cloacae |
| topic | Enterobacter cloacae Metabolic engineering Co-production Acetoin Succinic acid |
| url | https://doi.org/10.1186/s13068-021-01878-1 |
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