Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study
Pedunculate oak (<i>Quercus robur</i> L.) is an economically important forest-forming species in Poland that produces seeds that are sensitive to desiccation; therefore, short-lived seeds are classified as recalcitrant. Such seeds display active metabolism throughout storage. Acorns stor...
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MDPI AG
2022-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/8/756 |
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| author | Agnieszka Szuba Ewa Marzena Kalemba Mikołaj Krzysztof Wawrzyniak Jan Suszka Paweł Chmielarz |
| author_facet | Agnieszka Szuba Ewa Marzena Kalemba Mikołaj Krzysztof Wawrzyniak Jan Suszka Paweł Chmielarz |
| author_sort | Agnieszka Szuba |
| collection | DOAJ |
| description | Pedunculate oak (<i>Quercus robur</i> L.) is an economically important forest-forming species in Poland that produces seeds that are sensitive to desiccation; therefore, short-lived seeds are classified as recalcitrant. Such seeds display active metabolism throughout storage. Acorns stored under controlled conditions (moisture content of 40%, temperature −3 °C) maintain viability for up to 1.5–2 years. Meanwhile, oaks only produce large numbers of seeds every few years during so-called mast years. This results in a scarcity of good-quality seeds for continuous nursery production and restoration. The recalcitrant storage behavior and the requirements of foresters make it necessary to develop a new protocol for longer acorn storage at lower temperatures. Two storage temperatures were tested: −3 °C (currently used in forest practice) and −7 °C. Our results showed that acorns stored for six months exhibited deterioration and reduced germination capacity, as well as reduced seedling performance, particularly when acorns were stored at −7 °C. To elucidate the decrease in quality during storage, an untargeted metabolomics study was performed for the first time and supported with the analysis of carbohydrates and percentages of carbon (C) and nitrogen (N). Embryonic axes were characterized by a lower C:N ratio and higher hydration. A total of 1985 metabolites were detected, and 303 were successfully identified and quantified, revealing 44 known metabolites that displayed significantly up- or downregulated abundance. We demonstrated for the first time that the significant deterioration of seed germination potential, particularly in seeds stored at −7 °C, was accompanied by an increased abundance of phenolic compounds and carbohydrates but also amino acids and phosphorylated monosaccharides, particularly in the embryonic axes. The increased abundance of defense-related metabolites (1,2,4-Benzenetriol; BTO), products of ascorbic acid degradation (threonic and isothreonic acid), as well as antifreezing compounds (sugar alcohols, predominantly threitol), was reported in seed stored at −7 °C. We hypothesize that seed deterioration was caused by freezing stress experienced during six months of storage at −7 °C, a decline in antioxidative potential and the unsuccessful rerouting of the energy-production pathways. Additionally, our data are a good example of the application of high-throughput metabolomic tools in forest management. |
| first_indexed | 2024-03-09T12:57:55Z |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T12:57:55Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-75fc3c8b8f6f443e8e43beedd2a249032023-11-30T21:58:29ZengMDPI AGMetabolites2218-19892022-08-0112875610.3390/metabo12080756Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic StudyAgnieszka Szuba0Ewa Marzena Kalemba1Mikołaj Krzysztof Wawrzyniak2Jan Suszka3Paweł Chmielarz4Polish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62035 Kórnik, PolandPolish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62035 Kórnik, PolandPolish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62035 Kórnik, PolandPolish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62035 Kórnik, PolandPolish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62035 Kórnik, PolandPedunculate oak (<i>Quercus robur</i> L.) is an economically important forest-forming species in Poland that produces seeds that are sensitive to desiccation; therefore, short-lived seeds are classified as recalcitrant. Such seeds display active metabolism throughout storage. Acorns stored under controlled conditions (moisture content of 40%, temperature −3 °C) maintain viability for up to 1.5–2 years. Meanwhile, oaks only produce large numbers of seeds every few years during so-called mast years. This results in a scarcity of good-quality seeds for continuous nursery production and restoration. The recalcitrant storage behavior and the requirements of foresters make it necessary to develop a new protocol for longer acorn storage at lower temperatures. Two storage temperatures were tested: −3 °C (currently used in forest practice) and −7 °C. Our results showed that acorns stored for six months exhibited deterioration and reduced germination capacity, as well as reduced seedling performance, particularly when acorns were stored at −7 °C. To elucidate the decrease in quality during storage, an untargeted metabolomics study was performed for the first time and supported with the analysis of carbohydrates and percentages of carbon (C) and nitrogen (N). Embryonic axes were characterized by a lower C:N ratio and higher hydration. A total of 1985 metabolites were detected, and 303 were successfully identified and quantified, revealing 44 known metabolites that displayed significantly up- or downregulated abundance. We demonstrated for the first time that the significant deterioration of seed germination potential, particularly in seeds stored at −7 °C, was accompanied by an increased abundance of phenolic compounds and carbohydrates but also amino acids and phosphorylated monosaccharides, particularly in the embryonic axes. The increased abundance of defense-related metabolites (1,2,4-Benzenetriol; BTO), products of ascorbic acid degradation (threonic and isothreonic acid), as well as antifreezing compounds (sugar alcohols, predominantly threitol), was reported in seed stored at −7 °C. We hypothesize that seed deterioration was caused by freezing stress experienced during six months of storage at −7 °C, a decline in antioxidative potential and the unsuccessful rerouting of the energy-production pathways. Additionally, our data are a good example of the application of high-throughput metabolomic tools in forest management.https://www.mdpi.com/2218-1989/12/8/756long-term storageacorn damagehigh individual variabilityfreezing stressmetabolic activation |
| spellingShingle | Agnieszka Szuba Ewa Marzena Kalemba Mikołaj Krzysztof Wawrzyniak Jan Suszka Paweł Chmielarz Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study Metabolites long-term storage acorn damage high individual variability freezing stress metabolic activation |
| title | Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study |
| title_full | Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study |
| title_fullStr | Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study |
| title_full_unstemmed | Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study |
| title_short | Deterioration in the Quality of Recalcitrant <i>Quercus robur</i> Seeds during Six Months of Storage at Subzero Temperatures: Ineffective Activation of Prosurvival Mechanisms and Evidence of Freezing Stress from an Untargeted Metabolomic Study |
| title_sort | deterioration in the quality of recalcitrant i quercus robur i seeds during six months of storage at subzero temperatures ineffective activation of prosurvival mechanisms and evidence of freezing stress from an untargeted metabolomic study |
| topic | long-term storage acorn damage high individual variability freezing stress metabolic activation |
| url | https://www.mdpi.com/2218-1989/12/8/756 |
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