Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion
Abstract Herein, the synthesis of a biocompatible silver nanoparticles (AgNPs), for colorimetric detection of toxic mercury (II) ion (Hg2+), is reported. Phenolic-rich fraction of Asystasia gangetica leaf was extracted and used as a reductant of silver salt, all within the hydrophilic konjac glucoma...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13384-x |
| _version_ | 1828330154560061440 |
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| author | Titilope John Jayeoye Fredrick Nwude Eze Opeyemi Joshua Olatunji Andrew Aondoaver Tyopine |
| author_facet | Titilope John Jayeoye Fredrick Nwude Eze Opeyemi Joshua Olatunji Andrew Aondoaver Tyopine |
| author_sort | Titilope John Jayeoye |
| collection | DOAJ |
| description | Abstract Herein, the synthesis of a biocompatible silver nanoparticles (AgNPs), for colorimetric detection of toxic mercury (II) ion (Hg2+), is reported. Phenolic-rich fraction of Asystasia gangetica leaf was extracted and used as a reductant of silver salt, all within the hydrophilic konjac glucomannan (KgM) solution as stabilizer, at room temperature (RT). The bioactive components of Asystasia gangetica phenolic extract (AGPE), as elucidated with a (UHPLC-MS-QTOF-MS), revealed plethora of phenolic compounds, which can facilitate the reduction of silver salt at ambient conditions. Sparkling yellow colloidal solution of KgM-AgNPs was realized within 1 h, at RT, having a UV–vis maximum at 420 nm. KgM-AgNPs was characterized using UV–vis, Raman and (FTIR), TEM, SEM, EDS, XRD, TGA/DTG. TEM and FESEM images showed that KgM-AgNPs were spherical, with particle size distribution around 10–15 nm from TEM. The KgM-AgNPs biocompatibility was investigated on mouse L929 fibrobroblast and rat erythrocytes, without any harmful damages on the tested cells. In aqueous environment, KgM-AgNPs demonstrated good detection capacity toward Hg2+, in a Hg2+ concentration dependent fashion, within 3 min. Absorbance ratios (A360/A408) was linear with Hg2+ concentrations from 0.010–10.0 to 10.0–60.0 µM, with an estimated (LOD) of 3.25 nM. The probe was applied in lake water sample, with satisfactory accuracy. |
| first_indexed | 2024-04-13T20:35:30Z |
| format | Article |
| id | doaj.art-75fc4c095401476fb955b1d8e8d044ec |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T20:35:30Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-75fc4c095401476fb955b1d8e8d044ec2022-12-22T02:31:03ZengNature PortfolioScientific Reports2045-23222022-06-0112111510.1038/s41598-022-13384-xSynthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ionTitilope John Jayeoye0Fredrick Nwude Eze1Opeyemi Joshua Olatunji2Andrew Aondoaver Tyopine3Department of Chemistry, Faculty of Physical Sciences, Alex-Ekwueme Federal UniversityFaculty of Pharmaceutical Sciences, Prince of Songkhla UniversityTraditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkhla UniversityDepartment of Chemistry, Faculty of Physical Sciences, Alex-Ekwueme Federal UniversityAbstract Herein, the synthesis of a biocompatible silver nanoparticles (AgNPs), for colorimetric detection of toxic mercury (II) ion (Hg2+), is reported. Phenolic-rich fraction of Asystasia gangetica leaf was extracted and used as a reductant of silver salt, all within the hydrophilic konjac glucomannan (KgM) solution as stabilizer, at room temperature (RT). The bioactive components of Asystasia gangetica phenolic extract (AGPE), as elucidated with a (UHPLC-MS-QTOF-MS), revealed plethora of phenolic compounds, which can facilitate the reduction of silver salt at ambient conditions. Sparkling yellow colloidal solution of KgM-AgNPs was realized within 1 h, at RT, having a UV–vis maximum at 420 nm. KgM-AgNPs was characterized using UV–vis, Raman and (FTIR), TEM, SEM, EDS, XRD, TGA/DTG. TEM and FESEM images showed that KgM-AgNPs were spherical, with particle size distribution around 10–15 nm from TEM. The KgM-AgNPs biocompatibility was investigated on mouse L929 fibrobroblast and rat erythrocytes, without any harmful damages on the tested cells. In aqueous environment, KgM-AgNPs demonstrated good detection capacity toward Hg2+, in a Hg2+ concentration dependent fashion, within 3 min. Absorbance ratios (A360/A408) was linear with Hg2+ concentrations from 0.010–10.0 to 10.0–60.0 µM, with an estimated (LOD) of 3.25 nM. The probe was applied in lake water sample, with satisfactory accuracy.https://doi.org/10.1038/s41598-022-13384-x |
| spellingShingle | Titilope John Jayeoye Fredrick Nwude Eze Opeyemi Joshua Olatunji Andrew Aondoaver Tyopine Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion Scientific Reports |
| title | Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion |
| title_full | Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion |
| title_fullStr | Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion |
| title_full_unstemmed | Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion |
| title_short | Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion |
| title_sort | synthesis of biocompatible konjac glucomannan stabilized silver nanoparticles with asystasia gangetica phenolic extract for colorimetric detection of mercury ii ion |
| url | https://doi.org/10.1038/s41598-022-13384-x |
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