Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease
The review summarizes the literature data on the studies of viral antigens and antibodies, specific to Ebola virus, suitable for the development of a test-system for immune-diagnostics of the disease caused by them. Represented are the results of investigations on the obtainment of hybridoma monoclo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2015-09-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/247 |
| _version_ | 1797220876039487488 |
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| author | E. I. Kazachinskaya Yu. V. Nikonorova V. B. Loktev |
| author_facet | E. I. Kazachinskaya Yu. V. Nikonorova V. B. Loktev |
| author_sort | E. I. Kazachinskaya |
| collection | DOAJ |
| description | The review summarizes the literature data on the studies of viral antigens and antibodies, specific to Ebola virus, suitable for the development of a test-system for immune-diagnostics of the disease caused by them. Represented are the results of investigations on the obtainment of hybridoma monoclonal antibodies (MCA), specific to structural viral proteins NP, VP40, and VP35; recombinant proteins NP, VP40, and VP35 in prokaryotic system of E. coli; as well as the possibility to utilize MCA panels for EV viral and recombinant proteins’ detection in ELISA. |
| first_indexed | 2024-03-08T13:43:28Z |
| format | Article |
| id | doaj.art-75fcc04e7ea943fd99900e284181eca0 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:30Z |
| publishDate | 2015-09-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-75fcc04e7ea943fd99900e284181eca02024-04-05T16:47:11ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2015-09-0103586410.21055/0370-1069-2015-3-58-64247Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus DiseaseE. I. Kazachinskaya0Yu. V. Nikonorova1V. B. Loktev2State Research Centre of Virology and Biotechnology “Vector”; Novosibirsk State UniversityState Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”; Institute of Cytology and Genetics of the RAS Siberian Branch; Novosibirsk State UniversityThe review summarizes the literature data on the studies of viral antigens and antibodies, specific to Ebola virus, suitable for the development of a test-system for immune-diagnostics of the disease caused by them. Represented are the results of investigations on the obtainment of hybridoma monoclonal antibodies (MCA), specific to structural viral proteins NP, VP40, and VP35; recombinant proteins NP, VP40, and VP35 in prokaryotic system of E. coli; as well as the possibility to utilize MCA panels for EV viral and recombinant proteins’ detection in ELISA.https://journal.microbe.ru/jour/article/view/247вирус эболарекомбинантные белкимоноклональные антителаиммунодиагностикаebola virusrecombinant proteinsmonoclonal antibodiesimmune-diagnostics |
| spellingShingle | E. I. Kazachinskaya Yu. V. Nikonorova V. B. Loktev Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease Проблемы особо опасных инфекций вирус эбола рекомбинантные белки моноклональные антитела иммунодиагностика ebola virus recombinant proteins monoclonal antibodies immune-diagnostics |
| title | Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease |
| title_full | Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease |
| title_fullStr | Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease |
| title_full_unstemmed | Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease |
| title_short | Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease |
| title_sort | monoclonal antibodies and recombinant proteins for immune diagnostics of ebola virus disease |
| topic | вирус эбола рекомбинантные белки моноклональные антитела иммунодиагностика ebola virus recombinant proteins monoclonal antibodies immune-diagnostics |
| url | https://journal.microbe.ru/jour/article/view/247 |
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