Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat
Aim: The histomophometric study of Fructus Psoralea (FP) on ethanol induced neurodegeneration of hippocampus was investigated in a rat by using micrometry. Material and Methods: The study was conducted on thirty healthy female adult wistar albino rats with regular 4-day estrus cycles. The exper...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2013-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/3217/8-%206596_E(N%20)_PF1(%20_%20)_F(H)_PF1(PUH)_PFA(H)_E(N)_PFA2(PP).pdf |
| Summary: | Aim: The histomophometric study of Fructus Psoralea (FP)
on ethanol induced neurodegeneration of hippocampus was
investigated in a rat by using micrometry.
Material and Methods: The study was conducted on thirty
healthy female adult wistar albino rats with regular 4-day estrus
cycles. The experiment was carried out for a period of 2–4
months. The rats were divided into- SHAM operated control
group (Group I) and experimental groups - overiectomised vehicle
control rat (Group II), OVX and orally treated with FP(Group III)
OVX and induced with ethanol (Group IV), OVX rats induced
with ethnaol and orally treated with FP (Group V). ANOVA tool
was used to test the mean positive behavioural activity of all the
groups. The diameter, packing density and the total number of
neurons were calculated from toluidine blue stained histological
section by using micrometry. The statistical package SPSS
(17.0 VERSION) was used. Statistical significance was defined
as p < 0.05. Data was expressed as mean ± SEM.
Discussion: In animal studies, ethanol was found to significantly
inhibit neuronal activity in the CA1 and CA3 pyramidal cell layers
of the hippocampus. FP increase the number of cholinergic
neurons in the basal forebrain which in turn leads to increased
function of hippocampus, a structure heavily implicated in
behavioural activity and memory consolidation.
Conclusion: People with extensive hippocampal damage
may experience amnesia, learning and memory disabilities.
Hence the herb FP may be used as an adjuvant to treat the
above neurological disorders. |
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| ISSN: | 2249-782X 0973-709X |