Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat
Aim: The histomophometric study of Fructus Psoralea (FP) on ethanol induced neurodegeneration of hippocampus was investigated in a rat by using micrometry. Material and Methods: The study was conducted on thirty healthy female adult wistar albino rats with regular 4-day estrus cycles. The exper...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2013-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/3217/8-%206596_E(N%20)_PF1(%20_%20)_F(H)_PF1(PUH)_PFA(H)_E(N)_PFA2(PP).pdf |
| _version_ | 1818203374263205888 |
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| author | Sivanandan Ramar Saraswathi P. |
| author_facet | Sivanandan Ramar Saraswathi P. |
| author_sort | Sivanandan Ramar |
| collection | DOAJ |
| description | Aim: The histomophometric study of Fructus Psoralea (FP)
on ethanol induced neurodegeneration of hippocampus was
investigated in a rat by using micrometry.
Material and Methods: The study was conducted on thirty
healthy female adult wistar albino rats with regular 4-day estrus
cycles. The experiment was carried out for a period of 2–4
months. The rats were divided into- SHAM operated control
group (Group I) and experimental groups - overiectomised vehicle
control rat (Group II), OVX and orally treated with FP(Group III)
OVX and induced with ethanol (Group IV), OVX rats induced
with ethnaol and orally treated with FP (Group V). ANOVA tool
was used to test the mean positive behavioural activity of all the
groups. The diameter, packing density and the total number of
neurons were calculated from toluidine blue stained histological
section by using micrometry. The statistical package SPSS
(17.0 VERSION) was used. Statistical significance was defined
as p < 0.05. Data was expressed as mean ± SEM.
Discussion: In animal studies, ethanol was found to significantly
inhibit neuronal activity in the CA1 and CA3 pyramidal cell layers
of the hippocampus. FP increase the number of cholinergic
neurons in the basal forebrain which in turn leads to increased
function of hippocampus, a structure heavily implicated in
behavioural activity and memory consolidation.
Conclusion: People with extensive hippocampal damage
may experience amnesia, learning and memory disabilities.
Hence the herb FP may be used as an adjuvant to treat the
above neurological disorders. |
| first_indexed | 2024-12-12T03:24:20Z |
| format | Article |
| id | doaj.art-75ffb149d3c54edc8a97bb7861254cdd |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T03:24:20Z |
| publishDate | 2013-08-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-75ffb149d3c54edc8a97bb7861254cdd2022-12-22T00:40:05ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2013-08-01781561156410.7860/JCDR/2013/6596.3217Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in RatSivanandan Ramar0Saraswathi P.1Assistant Professor, Department of Anatomy, Madha Medical College and Research Institute, Chennai-600 122, India.Professor, Department of Anatomy, Saveetha University, Chennai-600 077, India.Aim: The histomophometric study of Fructus Psoralea (FP) on ethanol induced neurodegeneration of hippocampus was investigated in a rat by using micrometry. Material and Methods: The study was conducted on thirty healthy female adult wistar albino rats with regular 4-day estrus cycles. The experiment was carried out for a period of 2–4 months. The rats were divided into- SHAM operated control group (Group I) and experimental groups - overiectomised vehicle control rat (Group II), OVX and orally treated with FP(Group III) OVX and induced with ethanol (Group IV), OVX rats induced with ethnaol and orally treated with FP (Group V). ANOVA tool was used to test the mean positive behavioural activity of all the groups. The diameter, packing density and the total number of neurons were calculated from toluidine blue stained histological section by using micrometry. The statistical package SPSS (17.0 VERSION) was used. Statistical significance was defined as p < 0.05. Data was expressed as mean ± SEM. Discussion: In animal studies, ethanol was found to significantly inhibit neuronal activity in the CA1 and CA3 pyramidal cell layers of the hippocampus. FP increase the number of cholinergic neurons in the basal forebrain which in turn leads to increased function of hippocampus, a structure heavily implicated in behavioural activity and memory consolidation. Conclusion: People with extensive hippocampal damage may experience amnesia, learning and memory disabilities. Hence the herb FP may be used as an adjuvant to treat the above neurological disorders.https://jcdr.net/articles/PDF/3217/8-%206596_E(N%20)_PF1(%20_%20)_F(H)_PF1(PUH)_PFA(H)_E(N)_PFA2(PP).pdffructus psoraleae (fp)cornu ammonis (ca)hippocampusamnesia |
| spellingShingle | Sivanandan Ramar Saraswathi P. Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat Journal of Clinical and Diagnostic Research fructus psoraleae (fp) cornu ammonis (ca) hippocampus amnesia |
| title | Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat |
| title_full | Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat |
| title_fullStr | Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat |
| title_full_unstemmed | Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat |
| title_short | Histomorphometric Study of Fructus Psoralea on Ethanol Induced Neurodegeneration of Hippocampus in Rat |
| title_sort | histomorphometric study of fructus psoralea on ethanol induced neurodegeneration of hippocampus in rat |
| topic | fructus psoraleae (fp) cornu ammonis (ca) hippocampus amnesia |
| url | https://jcdr.net/articles/PDF/3217/8-%206596_E(N%20)_PF1(%20_%20)_F(H)_PF1(PUH)_PFA(H)_E(N)_PFA2(PP).pdf |
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