Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i>
<i>Acinetobacter baumannii</i> is a well-known nosocomial pathogen that can survive in different environments through the use of intricate networks to regulate gene expression. Two-component systems (TCS) form an important part of such regulatory networks, and in this study, we describe...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12606 |
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| author | Hung-Yu Shu Yu-Wen Huang Ping-Yi Tsai Kun-Sheng Hsieh Guang-Huey Lin |
| author_facet | Hung-Yu Shu Yu-Wen Huang Ping-Yi Tsai Kun-Sheng Hsieh Guang-Huey Lin |
| author_sort | Hung-Yu Shu |
| collection | DOAJ |
| description | <i>Acinetobacter baumannii</i> is a well-known nosocomial pathogen that can survive in different environments through the use of intricate networks to regulate gene expression. Two-component systems (TCS) form an important part of such regulatory networks, and in this study, we describe the identification and characterization of a novel EmaSR TCS in <i>A. baumannii</i>. We constructed a Tn<i>5</i>-tagged mutagenesis library, from which an <i>emaS</i> sensor kinase gene and <i>emaR</i> response regulator gene were identified. We found that <i>emaS</i>/<i>emaR</i> single-mutants and double-mutants were unable to replicate in M9 medium with 1% ethanol as the single carbon source. Motility and biofilm formation were negatively affected in double-mutants, and transcriptomic analysis showed that mutation of <i>emaSR</i> dysregulated genes required for carbon metabolism. In addition, <i>emaS</i>/<i>emaR</i> single-mutants and double-mutants were unable to survive in acetic acid- and sodium acetate-containing medium, indicating that the EmaSR TCS is also important for acetate metabolism. Furthermore, virulence against <i>Galleria mellonella</i> was diminished in <i>emaS</i>/<i>emaR</i> single- and double-mutants. Taken together, these results show that this novel EmaSR TCS is involved in the regulation of <i>A. baumannii</i> ethanol metabolism and acetate metabolism, with important implications on motility, biofilm formation, and virulence if mutated. Further research on the underlying mechanisms is warranted. |
| first_indexed | 2024-03-09T20:04:50Z |
| format | Article |
| id | doaj.art-75fff84a8a614ffc82eb3f71240fecf4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:04:50Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-75fff84a8a614ffc82eb3f71240fecf42023-11-24T00:34:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201260610.3390/ijms232012606Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i>Hung-Yu Shu0Yu-Wen Huang1Ping-Yi Tsai2Kun-Sheng Hsieh3Guang-Huey Lin4Department of Bioscience Technology, Chang Jung Christian University, Tainan 711301, TaiwanDepartment of Life Sciences, School of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment of Microbiology and Immunology, School of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment of Microbiology and Immunology, School of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment of Microbiology and Immunology, School of Medicine, Tzu Chi University, Hualien 970374, Taiwan<i>Acinetobacter baumannii</i> is a well-known nosocomial pathogen that can survive in different environments through the use of intricate networks to regulate gene expression. Two-component systems (TCS) form an important part of such regulatory networks, and in this study, we describe the identification and characterization of a novel EmaSR TCS in <i>A. baumannii</i>. We constructed a Tn<i>5</i>-tagged mutagenesis library, from which an <i>emaS</i> sensor kinase gene and <i>emaR</i> response regulator gene were identified. We found that <i>emaS</i>/<i>emaR</i> single-mutants and double-mutants were unable to replicate in M9 medium with 1% ethanol as the single carbon source. Motility and biofilm formation were negatively affected in double-mutants, and transcriptomic analysis showed that mutation of <i>emaSR</i> dysregulated genes required for carbon metabolism. In addition, <i>emaS</i>/<i>emaR</i> single-mutants and double-mutants were unable to survive in acetic acid- and sodium acetate-containing medium, indicating that the EmaSR TCS is also important for acetate metabolism. Furthermore, virulence against <i>Galleria mellonella</i> was diminished in <i>emaS</i>/<i>emaR</i> single- and double-mutants. Taken together, these results show that this novel EmaSR TCS is involved in the regulation of <i>A. baumannii</i> ethanol metabolism and acetate metabolism, with important implications on motility, biofilm formation, and virulence if mutated. Further research on the underlying mechanisms is warranted.https://www.mdpi.com/1422-0067/23/20/12606<i>Acinetobacter baumannii</i>two-component systemalcohol metabolismacetate metabolismbacterial motilitybiofilm formation |
| spellingShingle | Hung-Yu Shu Yu-Wen Huang Ping-Yi Tsai Kun-Sheng Hsieh Guang-Huey Lin Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i> International Journal of Molecular Sciences <i>Acinetobacter baumannii</i> two-component system alcohol metabolism acetate metabolism bacterial motility biofilm formation |
| title | Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i> |
| title_full | Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i> |
| title_fullStr | Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i> |
| title_short | Role of EmaSR in Ethanol Metabolism by <i>Acinetobacter baumannii</i> |
| title_sort | role of emasr in ethanol metabolism by i acinetobacter baumannii i |
| topic | <i>Acinetobacter baumannii</i> two-component system alcohol metabolism acetate metabolism bacterial motility biofilm formation |
| url | https://www.mdpi.com/1422-0067/23/20/12606 |
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