A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells
Background: Hyperactivated airway mucosa cells overproduce mucin and cause severe breathing complications. Here, we aimed to identify the effects of saponins derived from Panax ginseng on inflammation and mucin overproduction. Methods: NCI–H292 cells were pre-incubated with 16 saponins derived from...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845322000859 |
| _version_ | 1797961209161449472 |
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| author | Hyojin Heo Yumin Kim Byungsun Cha Sofia Brito Haneul Kim Hyunjin Kim Bassiratou M. Fatombi So Young Jung So Min Lee Lei Lei Sang Hun Lee Geon-woo Park Byeong-Mun Kwak Bum-Ho Bin Ji-Hwan Park Mi-Gi Lee |
| author_facet | Hyojin Heo Yumin Kim Byungsun Cha Sofia Brito Haneul Kim Hyunjin Kim Bassiratou M. Fatombi So Young Jung So Min Lee Lei Lei Sang Hun Lee Geon-woo Park Byeong-Mun Kwak Bum-Ho Bin Ji-Hwan Park Mi-Gi Lee |
| author_sort | Hyojin Heo |
| collection | DOAJ |
| description | Background: Hyperactivated airway mucosa cells overproduce mucin and cause severe breathing complications. Here, we aimed to identify the effects of saponins derived from Panax ginseng on inflammation and mucin overproduction. Methods: NCI–H292 cells were pre-incubated with 16 saponins derived from P. ginseng, and mucin overproduction was induced by treatment with phorbol 12-myristate 13-acetate (PMA). Mucin protein MUC5AC was quantified by enzyme-linked immunosorbent assay, and mRNA levels were analyzed using quantitative polymerase chain reaction (qPCR). Moreover, we performed a transcriptome analysis of PMA-treated NCI–H292 cells in the absence or presence of Rg5, and differential gene expression was confirmed using qPCR. Phosphorylation levels of signaling molecules, and the abundance of lipid droplets, were measured by western blotting, flow cytometry, and confocal microscopy. Results: Ginsenoside Rg5 effectively reduced MUC5AC secretion and decreased MUC5AC mRNA levels. A systematic functional network analysis revealed that Rg5 upregulated cholesterol and glycerolipid metabolism, resulting in the production of lipid droplets to clear reactive oxygen species (ROS), and modulated the mitogen-activated protein kinase and nuclear factor (NF)-κB signaling pathways to regulate inflammatory responses. Rg5 induced the accumulation of lipid droplets and decreased cellular ROS levels, and N-acetyl-l-cysteine, a ROS inhibitor, reduced MUC5AC secretion via Rg5. Furthermore, Rg5 hampered the phosphorylation of extracellular signal-regulated kinase and p38 proteins, affecting the NF-κB signaling pathway and pro-inflammatory responses. Conclusion: Rg5 alleviated inflammatory responses by reducing mucin secretion and promoting lipid droplet-mediated ROS clearance. Therefore, Rg5 may have potential as a therapeutic agent to alleviate respiratory disorders caused by hyperactivation of mucosa cells. |
| first_indexed | 2024-04-11T00:55:30Z |
| format | Article |
| id | doaj.art-760128e1ee124f09a721699afafaad5e |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-11T00:55:30Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-760128e1ee124f09a721699afafaad5e2023-01-05T04:31:32ZengElsevierJournal of Ginseng Research1226-84532023-01-0147197105A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cellsHyojin Heo0Yumin Kim1Byungsun Cha2Sofia Brito3Haneul Kim4Hyunjin Kim5Bassiratou M. Fatombi6So Young Jung7So Min Lee8Lei Lei9Sang Hun Lee10Geon-woo Park11Byeong-Mun Kwak12Bum-Ho Bin13Ji-Hwan Park14Mi-Gi Lee15Department of Applied Biotechnology, Ajou University, Suwon, Republic of KoreaKorea Bioinformation Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of Korea; Department of Biomedical Science and Engineering, Gwangju Institute of Science & Technology, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Applied Biotechnology, Ajou University, Suwon, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Applied Biotechnology, Ajou University, Suwon, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Biological Sciences, Ajou University, Suwon, Republic of KoreaDepartment of Applied Biotechnology, Ajou University, Suwon, Republic of Korea; Korea Bioinformation Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of KoreaDepartment of Meridian and Acupoint, College of Korean Medicine, Semyung University, Chungbuk, Republic of Korea; Corresponding author. Department of Meridian and Acupoint, College of Korean Medicine, Semyung University, Chungbuk, 27136, Republic of Korea.Department of Applied Biotechnology, Ajou University, Suwon, Republic of Korea; Department of Biological Sciences, Ajou University, Suwon, Republic of Korea; Corresponding author. Department of Biological Sciences, Ajou University, Suwon, 16499, Republic of Korea.Korea Bioinformation Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of Korea; Corresponding author. Korea Bioinformation Center, Korea Research Institute of Bioscience & Biotechnology, Republic of Korea.Bio-Center, Gyeonggi-do Business and Science Accelerator, Suwon, Republic of Korea; Corresponding author. Bio-Center, Gyeonggi-do Business and Science Accelerator, Suwon, 16229, Republic of Korea.Background: Hyperactivated airway mucosa cells overproduce mucin and cause severe breathing complications. Here, we aimed to identify the effects of saponins derived from Panax ginseng on inflammation and mucin overproduction. Methods: NCI–H292 cells were pre-incubated with 16 saponins derived from P. ginseng, and mucin overproduction was induced by treatment with phorbol 12-myristate 13-acetate (PMA). Mucin protein MUC5AC was quantified by enzyme-linked immunosorbent assay, and mRNA levels were analyzed using quantitative polymerase chain reaction (qPCR). Moreover, we performed a transcriptome analysis of PMA-treated NCI–H292 cells in the absence or presence of Rg5, and differential gene expression was confirmed using qPCR. Phosphorylation levels of signaling molecules, and the abundance of lipid droplets, were measured by western blotting, flow cytometry, and confocal microscopy. Results: Ginsenoside Rg5 effectively reduced MUC5AC secretion and decreased MUC5AC mRNA levels. A systematic functional network analysis revealed that Rg5 upregulated cholesterol and glycerolipid metabolism, resulting in the production of lipid droplets to clear reactive oxygen species (ROS), and modulated the mitogen-activated protein kinase and nuclear factor (NF)-κB signaling pathways to regulate inflammatory responses. Rg5 induced the accumulation of lipid droplets and decreased cellular ROS levels, and N-acetyl-l-cysteine, a ROS inhibitor, reduced MUC5AC secretion via Rg5. Furthermore, Rg5 hampered the phosphorylation of extracellular signal-regulated kinase and p38 proteins, affecting the NF-κB signaling pathway and pro-inflammatory responses. Conclusion: Rg5 alleviated inflammatory responses by reducing mucin secretion and promoting lipid droplet-mediated ROS clearance. Therefore, Rg5 may have potential as a therapeutic agent to alleviate respiratory disorders caused by hyperactivation of mucosa cells.http://www.sciencedirect.com/science/article/pii/S1226845322000859Airway mucosa inflammationGinsenoside Rg5Mucin overproductionLipid dropletReactive oxygen species |
| spellingShingle | Hyojin Heo Yumin Kim Byungsun Cha Sofia Brito Haneul Kim Hyunjin Kim Bassiratou M. Fatombi So Young Jung So Min Lee Lei Lei Sang Hun Lee Geon-woo Park Byeong-Mun Kwak Bum-Ho Bin Ji-Hwan Park Mi-Gi Lee A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells Journal of Ginseng Research Airway mucosa inflammation Ginsenoside Rg5 Mucin overproduction Lipid droplet Reactive oxygen species |
| title | A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells |
| title_full | A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells |
| title_fullStr | A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells |
| title_full_unstemmed | A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells |
| title_short | A systematic exploration of ginsenoside Rg5 reveals anti-inflammatory functions in airway mucosa cells |
| title_sort | systematic exploration of ginsenoside rg5 reveals anti inflammatory functions in airway mucosa cells |
| topic | Airway mucosa inflammation Ginsenoside Rg5 Mucin overproduction Lipid droplet Reactive oxygen species |
| url | http://www.sciencedirect.com/science/article/pii/S1226845322000859 |
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