Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus
The main objective of this research was to determine the proper growth conditions of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus for the production of lactic acid using serum as substract. This serum was obtain from the department of Cesar, Colombia. Lactic acid is the...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Nacional de Colombia
2015-09-01
|
| Series: | Revista Colombiana de Química |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/rcolquim/article/view/55604 |
| _version_ | 1818115288411930624 |
|---|---|
| author | Adriana M. Rojas Liceth P. Montaño Marlon J. Bastidas |
| author_facet | Adriana M. Rojas Liceth P. Montaño Marlon J. Bastidas |
| author_sort | Adriana M. Rojas |
| collection | DOAJ |
| description | The main objective of this research was to determine the proper growth conditions of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus for the production of lactic acid using serum as substract. This serum was obtain from the department of Cesar, Colombia. Lactic acid is the result of the extraction and purification of fermentation broths in which bacteria Lactobacillus delbrueckii subsp bulgaricus and Streptococcus thermophilus are used, which are usually used for the production of yogurt. The substrate was supplemented with yeast extract, ammonium phosphate as a nitrogen source, and calcium carbonate as a neutralizer, in order to optimize the consumption, by the bacteria, of the main carbohydrate present in serum (lactose). During the fermentation (up to 72 h) the inoculums concentration, and temperature were controlled. Purification consisted in esterification, filtration of solids formed during the reaction, and removing of water by evaporation and nitrogen influx. Finally, lactic acid was obtained with 78,0% purity (36.7 g/L), which was characterized by infrared spectroscopy |
| first_indexed | 2024-12-11T04:04:14Z |
| format | Article |
| id | doaj.art-7605d4a3db8d4e73ae44b71b5a017172 |
| institution | Directory Open Access Journal |
| issn | 0120-2804 2357-3791 |
| language | English |
| last_indexed | 2024-12-11T04:04:14Z |
| publishDate | 2015-09-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Química |
| spelling | doaj.art-7605d4a3db8d4e73ae44b71b5a0171722022-12-22T01:21:33ZengUniversidad Nacional de ColombiaRevista Colombiana de Química0120-28042357-37912015-09-0144351010.15446/rev.colomb.quim.v44n3.5560442397Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilusAdriana M. Rojas0Liceth P. Montaño1Marlon J. Bastidas2Grupo Energías Alternativas y Biomasa (GEAB), Universidad Popular del Cesar Sede Hurtado.Grupo Energías Alternativas y Biomasa (GEAB), Universidad Popular del Cesar Sede Hurtado.Grupo Desarrollo de Estudios y Tecnologías Ambientales del Carbono, Universidad de La GuajiraThe main objective of this research was to determine the proper growth conditions of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus for the production of lactic acid using serum as substract. This serum was obtain from the department of Cesar, Colombia. Lactic acid is the result of the extraction and purification of fermentation broths in which bacteria Lactobacillus delbrueckii subsp bulgaricus and Streptococcus thermophilus are used, which are usually used for the production of yogurt. The substrate was supplemented with yeast extract, ammonium phosphate as a nitrogen source, and calcium carbonate as a neutralizer, in order to optimize the consumption, by the bacteria, of the main carbohydrate present in serum (lactose). During the fermentation (up to 72 h) the inoculums concentration, and temperature were controlled. Purification consisted in esterification, filtration of solids formed during the reaction, and removing of water by evaporation and nitrogen influx. Finally, lactic acid was obtained with 78,0% purity (36.7 g/L), which was characterized by infrared spectroscopyhttp://www.revistas.unal.edu.co/index.php/rcolquim/article/view/55604ácido lácticolactosuerobacterias acido lácticasLactobacillus delbrueckiiStreptococcus thermophilus |
| spellingShingle | Adriana M. Rojas Liceth P. Montaño Marlon J. Bastidas Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus Revista Colombiana de Química ácido láctico lactosuero bacterias acido lácticas Lactobacillus delbrueckii Streptococcus thermophilus |
| title | Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus |
| title_full | Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus |
| title_fullStr | Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus |
| title_full_unstemmed | Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus |
| title_short | Production of lactic acid from whey using Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus |
| title_sort | production of lactic acid from whey using lactobacillus delbrueckii subsp bulgaricus and streptococcus thermophilus |
| topic | ácido láctico lactosuero bacterias acido lácticas Lactobacillus delbrueckii Streptococcus thermophilus |
| url | http://www.revistas.unal.edu.co/index.php/rcolquim/article/view/55604 |
| work_keys_str_mv | AT adrianamrojas productionoflacticacidfromwheyusinglactobacillusdelbrueckiisubspbulgaricusandstreptococcusthermophilus AT licethpmontano productionoflacticacidfromwheyusinglactobacillusdelbrueckiisubspbulgaricusandstreptococcusthermophilus AT marlonjbastidas productionoflacticacidfromwheyusinglactobacillusdelbrueckiisubspbulgaricusandstreptococcusthermophilus |