Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress
Peanut (<i>Arachis hypogaea</i> L.) is a globally cultivated crop of significant economic and nutritional importance. The role of gibberellic-acid-stimulated Arabidopsis (GASA) family genes is well established in plant growth, development, and biotic and abiotic stress responses. However...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/17117 |
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| author | Rizwana Begum Syed Nabi Myoung Hee Lee Kwang-Soo Cho Rupesh Tayade Sungup Kim Jung-In Kim Min-Young Kim Eunsoo Lee Jungeun Lee Sang-Woo Kim Eunyoung Oh |
| author_facet | Rizwana Begum Syed Nabi Myoung Hee Lee Kwang-Soo Cho Rupesh Tayade Sungup Kim Jung-In Kim Min-Young Kim Eunsoo Lee Jungeun Lee Sang-Woo Kim Eunyoung Oh |
| author_sort | Rizwana Begum Syed Nabi |
| collection | DOAJ |
| description | Peanut (<i>Arachis hypogaea</i> L.) is a globally cultivated crop of significant economic and nutritional importance. The role of gibberellic-acid-stimulated Arabidopsis (GASA) family genes is well established in plant growth, development, and biotic and abiotic stress responses. However, there is a gap in understanding the function of GASA proteins in cultivated peanuts, particularly in response to abiotic stresses such as drought and salinity. Thus, we conducted comprehensive in silico analyses to identify and verify the existence of 40 GASA genes (termed <i>AhGASA</i>) in cultivated peanuts. Subsequently, we conducted biological experiments and performed expression analyses of selected <i>AhGASA</i> genes to elucidate their potential regulatory roles in response to drought and salinity. Phylogenetic analysis revealed that <i>AhGASA</i> genes could be categorized into four distinct subfamilies. Under normal growth conditions, selected <i>AhGASA</i> genes exhibited varying expressions in young peanut seedling leaves, stems, and roots tissues. Notably, our findings indicate that certain <i>AhGASA</i> genes were downregulated under drought stress but upregulated under salt stress. These results suggest that specific <i>AhGASA</i> genes are involved in the regulation of salt or drought stress. Further functional characterization of the upregulated genes under both drought and salt stress will be essential to confirm their regulatory roles in this context. Overall, our findings provide compelling evidence of the involvement of <i>AhGASA</i> genes in the mechanisms of stress tolerance in cultivated peanuts. This study enhances our understanding of the functions of <i>AhGASA</i> genes in response to abiotic stress and lays the groundwork for future investigations into the molecular characterization of <i>AhGASA</i> genes. |
| first_indexed | 2024-03-09T01:48:57Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:48:57Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-7607c306ee7e44e1b7ec878eeda8ae482023-12-08T15:18:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124231711710.3390/ijms242317117Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic StressRizwana Begum Syed Nabi0Myoung Hee Lee1Kwang-Soo Cho2Rupesh Tayade3Sungup Kim4Jung-In Kim5Min-Young Kim6Eunsoo Lee7Jungeun Lee8Sang-Woo Kim9Eunyoung Oh10Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Republic of KoreaPeanut (<i>Arachis hypogaea</i> L.) is a globally cultivated crop of significant economic and nutritional importance. The role of gibberellic-acid-stimulated Arabidopsis (GASA) family genes is well established in plant growth, development, and biotic and abiotic stress responses. However, there is a gap in understanding the function of GASA proteins in cultivated peanuts, particularly in response to abiotic stresses such as drought and salinity. Thus, we conducted comprehensive in silico analyses to identify and verify the existence of 40 GASA genes (termed <i>AhGASA</i>) in cultivated peanuts. Subsequently, we conducted biological experiments and performed expression analyses of selected <i>AhGASA</i> genes to elucidate their potential regulatory roles in response to drought and salinity. Phylogenetic analysis revealed that <i>AhGASA</i> genes could be categorized into four distinct subfamilies. Under normal growth conditions, selected <i>AhGASA</i> genes exhibited varying expressions in young peanut seedling leaves, stems, and roots tissues. Notably, our findings indicate that certain <i>AhGASA</i> genes were downregulated under drought stress but upregulated under salt stress. These results suggest that specific <i>AhGASA</i> genes are involved in the regulation of salt or drought stress. Further functional characterization of the upregulated genes under both drought and salt stress will be essential to confirm their regulatory roles in this context. Overall, our findings provide compelling evidence of the involvement of <i>AhGASA</i> genes in the mechanisms of stress tolerance in cultivated peanuts. This study enhances our understanding of the functions of <i>AhGASA</i> genes in response to abiotic stress and lays the groundwork for future investigations into the molecular characterization of <i>AhGASA</i> genes.https://www.mdpi.com/1422-0067/24/23/17117GASApeanutgibberellindroughtsalt stressphylogenetic analysis |
| spellingShingle | Rizwana Begum Syed Nabi Myoung Hee Lee Kwang-Soo Cho Rupesh Tayade Sungup Kim Jung-In Kim Min-Young Kim Eunsoo Lee Jungeun Lee Sang-Woo Kim Eunyoung Oh Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress International Journal of Molecular Sciences GASA peanut gibberellin drought salt stress phylogenetic analysis |
| title | Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress |
| title_full | Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress |
| title_fullStr | Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress |
| title_full_unstemmed | Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress |
| title_short | Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (<i>Arachis hypogaea</i> L.) under Abiotic Stress |
| title_sort | genome wide identification and comprehensive analysis of the gasa gene family in peanuts i arachis hypogaea i l under abiotic stress |
| topic | GASA peanut gibberellin drought salt stress phylogenetic analysis |
| url | https://www.mdpi.com/1422-0067/24/23/17117 |
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