Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for reproductive disorders in sows and respiratory problems in pigs, and has a major economic impact. Controlling PRRSV is therefore a priority for the swine industry. Stabilization of a herd, defined as t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Porcine Health Management |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40813-017-0058-1 |
| _version_ | 1828759741380165632 |
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| author | Pauline Berton Valérie Normand Guy-Pierre Martineau Franck Bouchet Arnaud Lebret Agnès Waret-Szkuta |
| author_facet | Pauline Berton Valérie Normand Guy-Pierre Martineau Franck Bouchet Arnaud Lebret Agnès Waret-Szkuta |
| author_sort | Pauline Berton |
| collection | DOAJ |
| description | Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for reproductive disorders in sows and respiratory problems in pigs, and has a major economic impact. Controlling PRRSV is therefore a priority for the swine industry. Stabilization of a herd, defined as the production of PRRSV-negative pigs at weaning from seropositive sows, is a common method of control, and different protocols have been described in the literature to achieve this stabilization. Context and purpose The objective of this study was to evaluate wether the combination of mass vaccination of sows and their piglets with a Genotype I modified live virus (MLV) vaccine, with temporal closure to the introduction of replacement animals and unidirectional pig and human flow can result in the production of PRRSV-negative pigs at weaning. The study took place in French farrow-to-finish farms located in a high-density swine area where the disease concerns over 60% of farms and only closely related strains of genotype I have been reported. Twenty-three 100-to-700 sow farrow-to-finish farms were selected prospectively between 2005 and 2014, regardless of their biosecurity level. Those farms adopted a stabilization protocol characterized by the following standardized measures: vaccination of sows, gilts, and piglets with the Genotype I MLV vaccine PORCILIS®PRRS, temporary herd closure, and strict internal biosecurity measures. Monitoring of herd status was then performed using a combination of 3 diagnostic tools: Real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and Open reading frame (ORF) 5 and ORF7 sequencing. The status of finishing units (either active or inactive, meaning PRRSV-positive or PRRSV-negative, respectively) was not considered in this study. Results and conclusions At the end of the monitoring period, considering the results of all the analyses, clinical signs, and epidemiology, 19 farms were considered stable and 1 remained unstable. In 3 farms it was commonly agreed to extend the number of vaccinated batches of piglets, which enabled them to be considered stable at the end of a second round of monitoring. The combination of vaccination of sows and their piglets with a Genotype I MLV vaccine, together with the closure of the farm and a unidirectional pig and human flow, seems to be effective for farrow–to-finish farms even in high-density swine area, even with French PRRSV strains closely related to one another. This research is the first European study examining such a large number of farms, and increased confidence in the results stems from the added value of using the ORF7 and ORF5 sequencing tool. |
| first_indexed | 2024-12-11T01:02:46Z |
| format | Article |
| id | doaj.art-7607e040c65c4790a3a5faf37fdba753 |
| institution | Directory Open Access Journal |
| issn | 2055-5660 |
| language | English |
| last_indexed | 2024-12-11T01:02:46Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Porcine Health Management |
| spelling | doaj.art-7607e040c65c4790a3a5faf37fdba7532022-12-22T01:26:17ZengBMCPorcine Health Management2055-56602017-05-01311710.1186/s40813-017-0058-1Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine areaPauline Berton0Valérie Normand1Guy-Pierre Martineau2Franck Bouchet3Arnaud Lebret4Agnès Waret-Szkuta5Porc. Spective, Chene Vert Conseil Veterinary GroupPorc. Spective, Chene Vert Conseil Veterinary GroupIHAP, Université de Toulouse, INRA, ENVTPorc. Spective, Chene Vert Conseil Veterinary GroupPorc. Spective, Chene Vert Conseil Veterinary GroupIHAP, Université de Toulouse, INRA, ENVTAbstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for reproductive disorders in sows and respiratory problems in pigs, and has a major economic impact. Controlling PRRSV is therefore a priority for the swine industry. Stabilization of a herd, defined as the production of PRRSV-negative pigs at weaning from seropositive sows, is a common method of control, and different protocols have been described in the literature to achieve this stabilization. Context and purpose The objective of this study was to evaluate wether the combination of mass vaccination of sows and their piglets with a Genotype I modified live virus (MLV) vaccine, with temporal closure to the introduction of replacement animals and unidirectional pig and human flow can result in the production of PRRSV-negative pigs at weaning. The study took place in French farrow-to-finish farms located in a high-density swine area where the disease concerns over 60% of farms and only closely related strains of genotype I have been reported. Twenty-three 100-to-700 sow farrow-to-finish farms were selected prospectively between 2005 and 2014, regardless of their biosecurity level. Those farms adopted a stabilization protocol characterized by the following standardized measures: vaccination of sows, gilts, and piglets with the Genotype I MLV vaccine PORCILIS®PRRS, temporary herd closure, and strict internal biosecurity measures. Monitoring of herd status was then performed using a combination of 3 diagnostic tools: Real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and Open reading frame (ORF) 5 and ORF7 sequencing. The status of finishing units (either active or inactive, meaning PRRSV-positive or PRRSV-negative, respectively) was not considered in this study. Results and conclusions At the end of the monitoring period, considering the results of all the analyses, clinical signs, and epidemiology, 19 farms were considered stable and 1 remained unstable. In 3 farms it was commonly agreed to extend the number of vaccinated batches of piglets, which enabled them to be considered stable at the end of a second round of monitoring. The combination of vaccination of sows and their piglets with a Genotype I MLV vaccine, together with the closure of the farm and a unidirectional pig and human flow, seems to be effective for farrow–to-finish farms even in high-density swine area, even with French PRRSV strains closely related to one another. This research is the first European study examining such a large number of farms, and increased confidence in the results stems from the added value of using the ORF7 and ORF5 sequencing tool.http://link.springer.com/article/10.1186/s40813-017-0058-1PRRSVStabilizationFranceFarrow-to-finishMLV vaccineGenotype I |
| spellingShingle | Pauline Berton Valérie Normand Guy-Pierre Martineau Franck Bouchet Arnaud Lebret Agnès Waret-Szkuta Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area Porcine Health Management PRRSV Stabilization France Farrow-to-finish MLV vaccine Genotype I |
| title | Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area |
| title_full | Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area |
| title_fullStr | Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area |
| title_full_unstemmed | Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area |
| title_short | Evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 French Farrow-to-finish farms located in a high-density swine area |
| title_sort | evaluation of porcine reproductive and respiratory syndrome stabilization protocols in 23 french farrow to finish farms located in a high density swine area |
| topic | PRRSV Stabilization France Farrow-to-finish MLV vaccine Genotype I |
| url | http://link.springer.com/article/10.1186/s40813-017-0058-1 |
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