Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study
The use of PDT in prosthodontics as a disinfection protocol can eradicate bacteria from tooth surfaces by causing the death of the microorganisms to which the photosensitizer binds, absorbing the energy of laser light during irradiation. The aim of the study was to investigate the capacity of PDT to...
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| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/1/144 |
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| author | Corina Elena Tisler Radu Chifor Mindra Eugenia Badea Marioara Moldovan Doina Prodan Rahela Carpa Stanca Cuc Ioana Chifor Alexandru Florin Badea |
| author_facet | Corina Elena Tisler Radu Chifor Mindra Eugenia Badea Marioara Moldovan Doina Prodan Rahela Carpa Stanca Cuc Ioana Chifor Alexandru Florin Badea |
| author_sort | Corina Elena Tisler |
| collection | DOAJ |
| description | The use of PDT in prosthodontics as a disinfection protocol can eradicate bacteria from tooth surfaces by causing the death of the microorganisms to which the photosensitizer binds, absorbing the energy of laser light during irradiation. The aim of the study was to investigate the capacity of PDT to increase the bond strength of full ceramic restorations. In this study, 45 extracted human teeth were prepared for veneers, crowns, and inlays and contaminated with <i>Streptococcus mutans</i>. Tooth surfaces decontamination was performed using a diode laser and methylene blue as a photosensitizer. The disinfection effect and the impact on tensile bond strength were evaluated by scanning electron microscopy (SEM) and pull-out tests of the cemented ceramic prosthesis. Results show that the number of bacteria was reduced from colonized prepared tooth surfaces, and the bond strength was increased when PDT was used. In conclusion, the present study indicates that using PDT as a protocol before the final adhesive cementation of ceramic restorations could be a promising approach, with outstanding advantages over conventional methods. |
| first_indexed | 2024-03-10T01:52:00Z |
| format | Article |
| id | doaj.art-7607fbb1da794f13acf1153139e66669 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T01:52:00Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-7607fbb1da794f13acf1153139e666692023-11-23T13:04:32ZengMDPI AGBiomedicines2227-90592022-01-0110114410.3390/biomedicines10010144Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro StudyCorina Elena Tisler0Radu Chifor1Mindra Eugenia Badea2Marioara Moldovan3Doina Prodan4Rahela Carpa5Stanca Cuc6Ioana Chifor7Alexandru Florin Badea8Department of Prosthetic Dentistry and Dental Materials, Iuliu Hatieganu University of Medicine and Pharmacy, 32 Clinicilor Street, 400006 Cluj-Napoca, RomaniaDepartment of Preventive Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Avram Iancu 31, 400083 Cluj-Napoca, RomaniaDepartment of Preventive Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Avram Iancu 31, 400083 Cluj-Napoca, RomaniaDepartment of Polymer Composites, Institute of Chemistry “Raluca Ripan”, University Babes-Bolyai, 400294 Cluj-Napoca, RomaniaDepartment of Polymer Composites, Institute of Chemistry “Raluca Ripan”, University Babes-Bolyai, 400294 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeș Bolyai University, 1 M. Kogălniceanu Street, 400084 Cluj-Napoca, RomaniaDepartment of Polymer Composites, Institute of Chemistry “Raluca Ripan”, University Babes-Bolyai, 400294 Cluj-Napoca, RomaniaDepartment of Preventive Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Avram Iancu 31, 400083 Cluj-Napoca, RomaniaDepartment of Morphological Sciences, Discipline of Anatomy and Embryology, Faculty of General Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 3–5 Clinicilor Street, 400006 Cluj-Napoca, RomaniaThe use of PDT in prosthodontics as a disinfection protocol can eradicate bacteria from tooth surfaces by causing the death of the microorganisms to which the photosensitizer binds, absorbing the energy of laser light during irradiation. The aim of the study was to investigate the capacity of PDT to increase the bond strength of full ceramic restorations. In this study, 45 extracted human teeth were prepared for veneers, crowns, and inlays and contaminated with <i>Streptococcus mutans</i>. Tooth surfaces decontamination was performed using a diode laser and methylene blue as a photosensitizer. The disinfection effect and the impact on tensile bond strength were evaluated by scanning electron microscopy (SEM) and pull-out tests of the cemented ceramic prosthesis. Results show that the number of bacteria was reduced from colonized prepared tooth surfaces, and the bond strength was increased when PDT was used. In conclusion, the present study indicates that using PDT as a protocol before the final adhesive cementation of ceramic restorations could be a promising approach, with outstanding advantages over conventional methods.https://www.mdpi.com/2227-9059/10/1/144photodynamic therapybiofilmceramicadhesionpull-out testSEM |
| spellingShingle | Corina Elena Tisler Radu Chifor Mindra Eugenia Badea Marioara Moldovan Doina Prodan Rahela Carpa Stanca Cuc Ioana Chifor Alexandru Florin Badea Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study Biomedicines photodynamic therapy biofilm ceramic adhesion pull-out test SEM |
| title | Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study |
| title_full | Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study |
| title_fullStr | Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study |
| title_full_unstemmed | Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study |
| title_short | Photodynamic Therapy (PDT) in Prosthodontics: Disinfection of Human Teeth Exposed to <i>Streptococcus mutans</i> and the Effect on the Adhesion of Full Ceramic Veneers, Crowns, and Inlays: An In Vitro Study |
| title_sort | photodynamic therapy pdt in prosthodontics disinfection of human teeth exposed to i streptococcus mutans i and the effect on the adhesion of full ceramic veneers crowns and inlays an in vitro study |
| topic | photodynamic therapy biofilm ceramic adhesion pull-out test SEM |
| url | https://www.mdpi.com/2227-9059/10/1/144 |
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