Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation
The efficacy of pyriproxyfen, a juvenile hormone analog (JHA), was evaluated using third instar larvae of Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae). Various doses of the compound, ranging from 0.01 to 2 ng/larva, were applied topically to larvae (12 h before pupariation). Treatme...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2015-11-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201504-0007_Evaluation_of_pyriproxyfen_a_juvenile_hormone_analog_on_Drosophila_melanogaster_Diptera_Drosophilidae_Ins.php |
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| author | Fethi BENSEBAA Samira KILANI-MORAKCHI Nadia ARIBI Noureddine SOLTANI |
| author_facet | Fethi BENSEBAA Samira KILANI-MORAKCHI Nadia ARIBI Noureddine SOLTANI |
| author_sort | Fethi BENSEBAA |
| collection | DOAJ |
| description | The efficacy of pyriproxyfen, a juvenile hormone analog (JHA), was evaluated using third instar larvae of Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae). Various doses of the compound, ranging from 0.01 to 2 ng/larva, were applied topically to larvae (12 h before pupariation). Treatment did not prevent pupariation but inhibited adult emergence at all the doses tested. In a second series of experiments the ecdysteroid content of pupae was determined following application of pyriproxyfen at two doses, 0.108 and 0.29 ng/larva, corresponding to ID25 and ID50, the doses required for 25 and 50% inhibition of adult emergence, respectively. Pyriproxyfen treatment increased the duration of pupal development. In addition, enzyme immunoassay measurements of ecdysteroids in whole body extracts of pupae indicated that pyriproxyfen decreased the ecdysteroid content in a dose-dependent manner. Finally, the effects on the cuticle of pyriproxyfen (ID50) were studied histologically, which revealed that this compound increased the thickness of the new adult cuticle and suppressed the formation of bristles. Biochemical analyzes revealed that an increase in chitin content of the cuticle is only recorded at the highest dose. Thus, a topical application of pyriproxyfen to third instar larvae interfered with the molting hormone and disrupted the normal development of this insect. |
| first_indexed | 2024-12-21T10:52:52Z |
| format | Article |
| id | doaj.art-76091ed8bedb49e5bda428605fd28bf6 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-21T10:52:52Z |
| publishDate | 2015-11-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-76091ed8bedb49e5bda428605fd28bf62022-12-21T19:06:37ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292015-11-01112462563110.14411/eje.2015.084eje-201504-0007Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formationFethi BENSEBAA0Samira KILANI-MORAKCHI1Nadia ARIBI2Noureddine SOLTANI3Laboratory of Applied Animal Biology, Department of Biology, Faculty of Sciences, Badji Mokhtar University of Annaba, 23000-Annaba, Algeria; e-mails: fethi.bensebaa@gmail.com; samira.morakchi@univ-annaba.dz; nadia.aribi@univ-annaba.dz; noureddine.soltani@univ-annaba.dzLaboratory of Applied Animal Biology, Department of Biology, Faculty of Sciences, Badji Mokhtar University of Annaba, 23000-Annaba, Algeria; e-mails: fethi.bensebaa@gmail.com; samira.morakchi@univ-annaba.dz; nadia.aribi@univ-annaba.dz; noureddine.soltani@univ-annaba.dzLaboratory of Applied Animal Biology, Department of Biology, Faculty of Sciences, Badji Mokhtar University of Annaba, 23000-Annaba, Algeria; e-mails: fethi.bensebaa@gmail.com; samira.morakchi@univ-annaba.dz; nadia.aribi@univ-annaba.dz; noureddine.soltani@univ-annaba.dzLaboratory of Applied Animal Biology, Department of Biology, Faculty of Sciences, Badji Mokhtar University of Annaba, 23000-Annaba, Algeria; e-mails: fethi.bensebaa@gmail.com; samira.morakchi@univ-annaba.dz; nadia.aribi@univ-annaba.dz; noureddine.soltani@univ-annaba.dzThe efficacy of pyriproxyfen, a juvenile hormone analog (JHA), was evaluated using third instar larvae of Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae). Various doses of the compound, ranging from 0.01 to 2 ng/larva, were applied topically to larvae (12 h before pupariation). Treatment did not prevent pupariation but inhibited adult emergence at all the doses tested. In a second series of experiments the ecdysteroid content of pupae was determined following application of pyriproxyfen at two doses, 0.108 and 0.29 ng/larva, corresponding to ID25 and ID50, the doses required for 25 and 50% inhibition of adult emergence, respectively. Pyriproxyfen treatment increased the duration of pupal development. In addition, enzyme immunoassay measurements of ecdysteroids in whole body extracts of pupae indicated that pyriproxyfen decreased the ecdysteroid content in a dose-dependent manner. Finally, the effects on the cuticle of pyriproxyfen (ID50) were studied histologically, which revealed that this compound increased the thickness of the new adult cuticle and suppressed the formation of bristles. Biochemical analyzes revealed that an increase in chitin content of the cuticle is only recorded at the highest dose. Thus, a topical application of pyriproxyfen to third instar larvae interfered with the molting hormone and disrupted the normal development of this insect.https://www.eje.cz/artkey/eje-201504-0007_Evaluation_of_pyriproxyfen_a_juvenile_hormone_analog_on_Drosophila_melanogaster_Diptera_Drosophilidae_Ins.phpdipteradrosophilidaepyriproxyfendrosophila melanogastertoxicityecdysteroidscuticlechitin |
| spellingShingle | Fethi BENSEBAA Samira KILANI-MORAKCHI Nadia ARIBI Noureddine SOLTANI Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation European Journal of Entomology diptera drosophilidae pyriproxyfen drosophila melanogaster toxicity ecdysteroids cuticle chitin |
| title | Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation |
| title_full | Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation |
| title_fullStr | Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation |
| title_full_unstemmed | Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation |
| title_short | Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation |
| title_sort | evaluation of pyriproxyfen a juvenile hormone analog on drosophila melanogaster diptera drosophilidae insecticidal activity ecdysteroid contents and cuticle formation |
| topic | diptera drosophilidae pyriproxyfen drosophila melanogaster toxicity ecdysteroids cuticle chitin |
| url | https://www.eje.cz/artkey/eje-201504-0007_Evaluation_of_pyriproxyfen_a_juvenile_hormone_analog_on_Drosophila_melanogaster_Diptera_Drosophilidae_Ins.php |
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