BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma
Background: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. Objective: The goal of this research was to determine the prevalence of the BRAF V...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2016-04-01
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| Series: | Case Reports in Oncology |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/445939 |
| _version_ | 1818878323847069696 |
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| author | Priscilla Denise Zepeda-Lopez Julio Cesar Salas-Alanis Sonia Toussaint-Caire Daniela Gutierrez-Mendoza Elisa Vega-Memije Saúl Lino Silva Oscar Raul Fajardo-Ramírez Gregorio Alcazar María Guadalupe Moreno-Treviño Hugo Alberto Barrera Saldaña |
| author_facet | Priscilla Denise Zepeda-Lopez Julio Cesar Salas-Alanis Sonia Toussaint-Caire Daniela Gutierrez-Mendoza Elisa Vega-Memije Saúl Lino Silva Oscar Raul Fajardo-Ramírez Gregorio Alcazar María Guadalupe Moreno-Treviño Hugo Alberto Barrera Saldaña |
| author_sort | Priscilla Denise Zepeda-Lopez |
| collection | DOAJ |
| description | Background: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. Objective: The goal of this research was to determine the prevalence of the BRAF V600E mutation in patients from Central Mexico diagnosed with primary melanoma. Methods: Skin biopsies from 47 patients with melanoma were obtained from the dermatology department of the Hospital General ‘Dr. Manuel Gea González' in Mexico City. For BRAF mutation determination, after DNA isolation, the gene region where the mutation occurs was amplified by PCR. Subsequently, the presence or absence of the V600E mutation was detected by Sanger sequencing performed at the private molecular diagnostic laboratory Vitagénesis in Monterrey, Mexico. Results: Of the 47 patients sampled, 6.4% harbored the V600E mutation. No statistical significance was found between mutations and the type of tumor. |
| first_indexed | 2024-12-19T14:12:22Z |
| format | Article |
| id | doaj.art-760ab20b304344f0ac64d01142ad6440 |
| institution | Directory Open Access Journal |
| issn | 1662-6575 |
| language | English |
| last_indexed | 2024-12-19T14:12:22Z |
| publishDate | 2016-04-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Case Reports in Oncology |
| spelling | doaj.art-760ab20b304344f0ac64d01142ad64402022-12-21T20:18:05ZengKarger PublishersCase Reports in Oncology1662-65752016-04-019124124510.1159/000445939445939BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with MelanomaPriscilla Denise Zepeda-LopezJulio Cesar Salas-AlanisSonia Toussaint-CaireDaniela Gutierrez-MendozaElisa Vega-MemijeSaúl Lino SilvaOscar Raul Fajardo-RamírezGregorio AlcazarMaría Guadalupe Moreno-TreviñoHugo Alberto Barrera SaldañaBackground: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. Objective: The goal of this research was to determine the prevalence of the BRAF V600E mutation in patients from Central Mexico diagnosed with primary melanoma. Methods: Skin biopsies from 47 patients with melanoma were obtained from the dermatology department of the Hospital General ‘Dr. Manuel Gea González' in Mexico City. For BRAF mutation determination, after DNA isolation, the gene region where the mutation occurs was amplified by PCR. Subsequently, the presence or absence of the V600E mutation was detected by Sanger sequencing performed at the private molecular diagnostic laboratory Vitagénesis in Monterrey, Mexico. Results: Of the 47 patients sampled, 6.4% harbored the V600E mutation. No statistical significance was found between mutations and the type of tumor.http://www.karger.com/Article/FullText/445939Mexican patientsMelanomaBRAFV600E mutation |
| spellingShingle | Priscilla Denise Zepeda-Lopez Julio Cesar Salas-Alanis Sonia Toussaint-Caire Daniela Gutierrez-Mendoza Elisa Vega-Memije Saúl Lino Silva Oscar Raul Fajardo-Ramírez Gregorio Alcazar María Guadalupe Moreno-Treviño Hugo Alberto Barrera Saldaña BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma Case Reports in Oncology Mexican patients Melanoma BRAF V600E mutation |
| title | BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma |
| title_full | BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma |
| title_fullStr | BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma |
| title_full_unstemmed | BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma |
| title_short | BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma |
| title_sort | braf mutation v600e prevalence in mexican patients diagnosed with melanoma |
| topic | Mexican patients Melanoma BRAF V600E mutation |
| url | http://www.karger.com/Article/FullText/445939 |
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