Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias

Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias

Previously we showed that 2D template matching (2DTM) can be used to localize macromolecular complexes in images recorded by cryogenic electron microscopy (cryo-EM) with high precision, even in the presence of noise and cellular background (Lucas et al., 2021; Lucas et al., 2022). Here, we show that...

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Bibliographic Details
Main Authors: Bronwyn A Lucas, Benjamin A Himes, Nikolaus Grigorieff
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2023-11-01
Series:eLife
Subjects:
electron microscopy
image analysis
ribosome
cryo-EM
3D structure
in situ
Online Access:https://elifesciences.org/articles/90486

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