Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan
Plasmodium vivax and Plasmodium falciparum are becoming resistant to drugs including antifolates, sulphonamides and chloroquine. This study was focused at sequence analysis of resistant genes of these parasites against sulphadoxine–pyrimethamine and chloroquine, from Bannu, Pakistan. Known mutations...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-09-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867013001499 |
| _version_ | 1818145610688102400 |
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| author | Lubna Khatoon Frederick N. Baliraine Salman A. Malik Guiyun Yan |
| author_facet | Lubna Khatoon Frederick N. Baliraine Salman A. Malik Guiyun Yan |
| author_sort | Lubna Khatoon |
| collection | DOAJ |
| description | Plasmodium vivax and Plasmodium falciparum are becoming resistant to drugs including antifolates, sulphonamides and chloroquine. This study was focused at sequence analysis of resistant genes of these parasites against sulphadoxine–pyrimethamine and chloroquine, from Bannu, Pakistan. Known mutations were detected at codons 57, 58 and 117 of pvdhfr gene of P. vivax, while none of the isolates had any pvdhps mutation. Similarly P. falciparum isolates exhibited double 59R + 108N mutations in pfdhfr, and single 437G in pfdhps thus demonstrating the existance of triple mutant 59R + 108N + 437G haplotype in this region. The key chloroquine resistance mutation, 76T in pfcrt was observed in 100% of the P. falciparum isolates, with haplotype SVMNT which is also associated with resistance to amodiaquine. Some novel mutations were also observed in pvdhfr and pfdhfr genes. Keywords: Plasmodium vivax, Plasmodium falciparum, Sequence analysis, Pakistan |
| first_indexed | 2024-12-11T12:06:12Z |
| format | Article |
| id | doaj.art-760cc3130ff84ce3a82527a7c2055a7a |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-11T12:06:12Z |
| publishDate | 2013-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-760cc3130ff84ce3a82527a7c2055a7a2022-12-22T01:07:56ZengElsevierBrazilian Journal of Infectious Diseases1413-86702013-09-01175596600Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of PakistanLubna Khatoon0Frederick N. Baliraine1Salman A. Malik2Guiyun Yan3Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Corresponding author at: Quaid-i-Azam University, PO Box 1090, Islamabad 45320, Pakistan.Department of Biology, LeTourneau University, Longview, TX, USADepartment of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, PakistanCollege of Health Sciences, University of California, Irvine, CA, USAPlasmodium vivax and Plasmodium falciparum are becoming resistant to drugs including antifolates, sulphonamides and chloroquine. This study was focused at sequence analysis of resistant genes of these parasites against sulphadoxine–pyrimethamine and chloroquine, from Bannu, Pakistan. Known mutations were detected at codons 57, 58 and 117 of pvdhfr gene of P. vivax, while none of the isolates had any pvdhps mutation. Similarly P. falciparum isolates exhibited double 59R + 108N mutations in pfdhfr, and single 437G in pfdhps thus demonstrating the existance of triple mutant 59R + 108N + 437G haplotype in this region. The key chloroquine resistance mutation, 76T in pfcrt was observed in 100% of the P. falciparum isolates, with haplotype SVMNT which is also associated with resistance to amodiaquine. Some novel mutations were also observed in pvdhfr and pfdhfr genes. Keywords: Plasmodium vivax, Plasmodium falciparum, Sequence analysis, Pakistanhttp://www.sciencedirect.com/science/article/pii/S1413867013001499 |
| spellingShingle | Lubna Khatoon Frederick N. Baliraine Salman A. Malik Guiyun Yan Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan Brazilian Journal of Infectious Diseases |
| title | Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan |
| title_full | Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan |
| title_fullStr | Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan |
| title_full_unstemmed | Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan |
| title_short | Sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in P. falciparum and P. vivax isolates from the Bannu district of Pakistan |
| title_sort | sequence analysis of genes associated with resistance to chloroquine and sulphadoxine pyrimethamine in p falciparum and p vivax isolates from the bannu district of pakistan |
| url | http://www.sciencedirect.com/science/article/pii/S1413867013001499 |
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