Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida
Aeromonas salmonicida is an ancient fish pathogen. Lysozymes are important molecules in the innate immune system that fight bacterial infections. The expression characteristics of C-type lysozyme in crucian carp infected with A. salmonicida and its antibacterial effect against A. salmonicida had not...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024000756 |
| _version_ | 1797336896718766080 |
|---|---|
| author | Xiao-dong Ling Jianshu Lv Fu-ju Chen Xiao-tong Qin Mei-si Wu Feng Bai Hui-qiong Luo |
| author_facet | Xiao-dong Ling Jianshu Lv Fu-ju Chen Xiao-tong Qin Mei-si Wu Feng Bai Hui-qiong Luo |
| author_sort | Xiao-dong Ling |
| collection | DOAJ |
| description | Aeromonas salmonicida is an ancient fish pathogen. Lysozymes are important molecules in the innate immune system that fight bacterial infections. The expression characteristics of C-type lysozyme in crucian carp infected with A. salmonicida and its antibacterial effect against A. salmonicida had not been investigated. Thus, we used bioinformatics to analyze the gene and protein characteristics of C-type lysozymes in crucian carp. Changes in C-type lysozyme expression before and after crucian carp infection with A. salmonicida were detected, and the in vitro antibacterial effect of recombinant carp C-type lysozyme on A. salmonicida was validated. The results showed that the coding DNA sequence region of the lysozyme gene sequence was 438 bp long, encoding 145 amino acids and containing two conserved catalytic sites: Glu53 and Asp69. Phylogenetic analysis revealed that crucian carp C-type lysozymes clustered with Cyprinus carpio lysozyme C. After crucian carp were infected with A. salmonicida, the gene and protein expression of C-type lysozymes in the liver, spleen, kidney, and hindgut were significantly upregulated, with the liver showing the highest upregulation that was 15 times higher than that in the uninfected group. In addition, recombinant C-type lysozyme exhibited significant antibacterial activity against A. salmonicida, with an average inhibition zone radius of 0.92 cm when using 40 μg recombinant lysozyme. In conclusion, this study reveals the important role of C-type lysozymes in the innate immune response of crucian carp and provides a theoretical basis for preventing crucian carp infection with A. salmonicida. |
| first_indexed | 2024-03-08T09:01:34Z |
| format | Article |
| id | doaj.art-760cd49ec87e4e2293b44a1b8a38847e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:01:34Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-760cd49ec87e4e2293b44a1b8a38847e2024-02-01T06:34:30ZengElsevierHeliyon2405-84402024-01-01101e24044Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicidaXiao-dong Ling0Jianshu Lv1Fu-ju Chen2Xiao-tong Qin3Mei-si Wu4Feng Bai5Hui-qiong Luo6Corresponding author. College of Agriculture and Animal Husbandry, Qinghai University, 251# Ningda Road, Xining 810000, China.; College of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaAeromonas salmonicida is an ancient fish pathogen. Lysozymes are important molecules in the innate immune system that fight bacterial infections. The expression characteristics of C-type lysozyme in crucian carp infected with A. salmonicida and its antibacterial effect against A. salmonicida had not been investigated. Thus, we used bioinformatics to analyze the gene and protein characteristics of C-type lysozymes in crucian carp. Changes in C-type lysozyme expression before and after crucian carp infection with A. salmonicida were detected, and the in vitro antibacterial effect of recombinant carp C-type lysozyme on A. salmonicida was validated. The results showed that the coding DNA sequence region of the lysozyme gene sequence was 438 bp long, encoding 145 amino acids and containing two conserved catalytic sites: Glu53 and Asp69. Phylogenetic analysis revealed that crucian carp C-type lysozymes clustered with Cyprinus carpio lysozyme C. After crucian carp were infected with A. salmonicida, the gene and protein expression of C-type lysozymes in the liver, spleen, kidney, and hindgut were significantly upregulated, with the liver showing the highest upregulation that was 15 times higher than that in the uninfected group. In addition, recombinant C-type lysozyme exhibited significant antibacterial activity against A. salmonicida, with an average inhibition zone radius of 0.92 cm when using 40 μg recombinant lysozyme. In conclusion, this study reveals the important role of C-type lysozymes in the innate immune response of crucian carp and provides a theoretical basis for preventing crucian carp infection with A. salmonicida.http://www.sciencedirect.com/science/article/pii/S2405844024000756Crucian carpLysozymeAeromonas salmonicidaAntibacterial activityImmune response |
| spellingShingle | Xiao-dong Ling Jianshu Lv Fu-ju Chen Xiao-tong Qin Mei-si Wu Feng Bai Hui-qiong Luo Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida Heliyon Crucian carp Lysozyme Aeromonas salmonicida Antibacterial activity Immune response |
| title | Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida |
| title_full | Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida |
| title_fullStr | Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida |
| title_full_unstemmed | Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida |
| title_short | Expression characteristics and in vitro antibacterial properties of C-type lysozyme in crucian carp infected with Aeromonas salmonicida |
| title_sort | expression characteristics and in vitro antibacterial properties of c type lysozyme in crucian carp infected with aeromonas salmonicida |
| topic | Crucian carp Lysozyme Aeromonas salmonicida Antibacterial activity Immune response |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024000756 |
| work_keys_str_mv | AT xiaodongling expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida AT jianshulv expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida AT fujuchen expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida AT xiaotongqin expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida AT meisiwu expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida AT fengbai expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida AT huiqiongluo expressioncharacteristicsandinvitroantibacterialpropertiesofctypelysozymeincruciancarpinfectedwithaeromonassalmonicida |