<i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes
Most studies of <i>Fusarium</i> head blight (FHB) focused on wheat infection at anthesis. Less is known about infections at later stages. In this study, the effect of infection timing on the development of FHB and the distribution of fungal biomass and deoxynivalenol (DON) along wheat sp...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/79 |
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| author | Elias Alisaac Anna Rathgeb Petr Karlovsky Anne-Katrin Mahlein |
| author_facet | Elias Alisaac Anna Rathgeb Petr Karlovsky Anne-Katrin Mahlein |
| author_sort | Elias Alisaac |
| collection | DOAJ |
| description | Most studies of <i>Fusarium</i> head blight (FHB) focused on wheat infection at anthesis. Less is known about infections at later stages. In this study, the effect of infection timing on the development of FHB and the distribution of fungal biomass and deoxynivalenol (DON) along wheat spikes was investigated. Under greenhouse conditions, two wheat varieties were point-inoculated with <i>Fusarium graminearum</i> starting from anthesis until 25 days after anthesis. The fungus and fungal DNA were isolated from the centers and the bases of all the spikes but not from the tips for all inoculation times and both varieties. In each variety, the amount of fungal DNA and the content of DON and deoxynivalenol-3-glucoside (DON-3-G) were higher in the center than in the base for all inoculation times. A positive correlation was found between the content of fungal DNA and DON in the centers as well as the bases of both varieties. This study showed that <i>F. graminearum</i> grows downward within infected wheat spikes and that the accumulation of DON is largely confined to the colonized tissue. Moreover, <i>F. graminearum</i> was able to infect wheat kernels and cause contamination with mycotoxins even when inoculated 25 days after anthesis. |
| first_indexed | 2024-03-10T13:39:43Z |
| format | Article |
| id | doaj.art-760cda2ab231425abc60e2b5413e05de |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:39:43Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-760cda2ab231425abc60e2b5413e05de2023-11-21T03:11:16ZengMDPI AGMicroorganisms2076-26072020-12-01917910.3390/microorganisms9010079<i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat SpikesElias Alisaac0Anna Rathgeb1Petr Karlovsky2Anne-Katrin Mahlein3Institute of Crop Science and Resource Conservation (INRES), Plant Diseases and Plant Protection, University of Bonn, 53115 Bonn, GermanyMolecular Phytopathology and Mycotoxin Research, University of Goettingen, 37077 Goettingen, GermanyMolecular Phytopathology and Mycotoxin Research, University of Goettingen, 37077 Goettingen, GermanyInstitute of Sugar Beet Research (IfZ), 37079 Goettingen, GermanyMost studies of <i>Fusarium</i> head blight (FHB) focused on wheat infection at anthesis. Less is known about infections at later stages. In this study, the effect of infection timing on the development of FHB and the distribution of fungal biomass and deoxynivalenol (DON) along wheat spikes was investigated. Under greenhouse conditions, two wheat varieties were point-inoculated with <i>Fusarium graminearum</i> starting from anthesis until 25 days after anthesis. The fungus and fungal DNA were isolated from the centers and the bases of all the spikes but not from the tips for all inoculation times and both varieties. In each variety, the amount of fungal DNA and the content of DON and deoxynivalenol-3-glucoside (DON-3-G) were higher in the center than in the base for all inoculation times. A positive correlation was found between the content of fungal DNA and DON in the centers as well as the bases of both varieties. This study showed that <i>F. graminearum</i> grows downward within infected wheat spikes and that the accumulation of DON is largely confined to the colonized tissue. Moreover, <i>F. graminearum</i> was able to infect wheat kernels and cause contamination with mycotoxins even when inoculated 25 days after anthesis.https://www.mdpi.com/2076-2607/9/1/79FHB<i>Triticum aestivum</i>wheat scab<i>Fusarium graminearum</i>fungal biomassdeoxynivalenol |
| spellingShingle | Elias Alisaac Anna Rathgeb Petr Karlovsky Anne-Katrin Mahlein <i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes Microorganisms FHB <i>Triticum aestivum</i> wheat scab <i>Fusarium graminearum</i> fungal biomass deoxynivalenol |
| title | <i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes |
| title_full | <i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes |
| title_fullStr | <i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes |
| title_full_unstemmed | <i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes |
| title_short | <i>Fusarium</i> Head Blight: Effect of Infection Timing on Spread of <i>Fusarium graminearum</i> and Spatial Distribution of Deoxynivalenol within Wheat Spikes |
| title_sort | i fusarium i head blight effect of infection timing on spread of i fusarium graminearum i and spatial distribution of deoxynivalenol within wheat spikes |
| topic | FHB <i>Triticum aestivum</i> wheat scab <i>Fusarium graminearum</i> fungal biomass deoxynivalenol |
| url | https://www.mdpi.com/2076-2607/9/1/79 |
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