<i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital
An impaired coagulation process has been described in patients with severe or critical coronavirus disease (COVID-19). Nevertheless, the implication of coagulation-related genes has not been explored. We aimed to evaluate the impact of <i>F5</i> rs6025 and <i>SERPINE1</i> rs6...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/4/595 |
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| author | Ingrid Fricke-Galindo Ivette Buendia-Roldan Leslie Chavez-Galan Gloria Pérez-Rubio Rafael de Jesús Hernández-Zenteno Espiridión Ramos-Martinez Armando Zazueta-Márquez Felipe Reyes-Melendres Aimé Alarcón-Dionet Javier Guzmán-Vargas Omar Andrés Bravo-Gutiérrez Teresa Quintero-Puerta Ilse Adriana Gutiérrez-Pérez Alejandro Ortega-Martínez Enrique Ambrocio-Ortiz Karol J. Nava-Quiroz José Luis Bañuelos-Flores María Esther Jaime-Capetillo Mayra Mejía Jorge Rojas-Serrano Ramcés Falfán-Valencia |
| author_facet | Ingrid Fricke-Galindo Ivette Buendia-Roldan Leslie Chavez-Galan Gloria Pérez-Rubio Rafael de Jesús Hernández-Zenteno Espiridión Ramos-Martinez Armando Zazueta-Márquez Felipe Reyes-Melendres Aimé Alarcón-Dionet Javier Guzmán-Vargas Omar Andrés Bravo-Gutiérrez Teresa Quintero-Puerta Ilse Adriana Gutiérrez-Pérez Alejandro Ortega-Martínez Enrique Ambrocio-Ortiz Karol J. Nava-Quiroz José Luis Bañuelos-Flores María Esther Jaime-Capetillo Mayra Mejía Jorge Rojas-Serrano Ramcés Falfán-Valencia |
| author_sort | Ingrid Fricke-Galindo |
| collection | DOAJ |
| description | An impaired coagulation process has been described in patients with severe or critical coronavirus disease (COVID-19). Nevertheless, the implication of coagulation-related genes has not been explored. We aimed to evaluate the impact of <i>F5</i> rs6025 and <i>SERPINE1</i> rs6092 on invasive mechanical ventilation (IMV) requirement and the levels of coagulation proteins among patients with severe COVID-19. Four-hundred fifty-five patients with severe COVID-19 were genotyped using TaqMan assays. Coagulation-related proteins (P-Selectin, D-dimer, P-selectin glycoprotein ligand-1, tissue plasminogen activator [tPA], plasminogen activator inhibitor-1, and Factor IX) were assessed by cytometric bead arrays in one- and two-time determinations. Accordingly, <i>SERPINE1</i> rs6092, P-Selectin (GG 385 pg/mL vs. AG+AA 632 pg/mL, <i>p</i> = 0.0037), and tPA (GG 1858 pg/mL vs. AG+AA 2546 pg/mL, <i>p</i> = 0.0284) levels were different. Patients carrying the CT <i>F5</i>-rs6025 genotype exhibited lower levels of factor IX (CC 17,136 pg/mL vs. CT 10,247 pg/mL, <i>p</i> = 0.0355). Coagulation proteins were also different among IMV patients than non-IMV. PSGL-1 levels were significantly increased in the late stage of COVID-19 (>10 days). The frequencies of <i>F5</i> rs6025 and <i>SERPINE1</i> rs6092 variants were not different among IMV and non-IMV. The <i>SERPINE1</i> rs6092 variant is related to the impaired coagulation process in patients with COVID-19 severe. |
| first_indexed | 2024-03-09T11:08:09Z |
| format | Article |
| id | doaj.art-760edf645b574469a20cae9ad5a80b9e |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T11:08:09Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-760edf645b574469a20cae9ad5a80b9e2023-12-01T00:52:05ZengMDPI AGBiology2079-77372022-04-0111459510.3390/biology11040595<i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care HospitalIngrid Fricke-Galindo0Ivette Buendia-Roldan1Leslie Chavez-Galan2Gloria Pérez-Rubio3Rafael de Jesús Hernández-Zenteno4Espiridión Ramos-Martinez5Armando Zazueta-Márquez6Felipe Reyes-Melendres7Aimé Alarcón-Dionet8Javier Guzmán-Vargas9Omar Andrés Bravo-Gutiérrez10Teresa Quintero-Puerta11Ilse Adriana Gutiérrez-Pérez12Alejandro Ortega-Martínez13Enrique Ambrocio-Ortiz14Karol J. Nava-Quiroz15José Luis Bañuelos-Flores16María Esther Jaime-Capetillo17Mayra Mejía18Jorge Rojas-Serrano19Ramcés Falfán-Valencia20HLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoTranslational Research Laboratory on Aging and Pulmonary Fibrosis, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City 14080, MexicoLaboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoCOPD Clinic, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoUnidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 06720, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoTranslational Research Laboratory on Aging and Pulmonary Fibrosis, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoClinical Laboratory Service, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoClinical Laboratory Service, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoInterstitial Pulmonary Diseases and Rheumatology Unit, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City 06720, MexicoInterstitial Pulmonary Diseases and Rheumatology Unit, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City 06720, MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoAn impaired coagulation process has been described in patients with severe or critical coronavirus disease (COVID-19). Nevertheless, the implication of coagulation-related genes has not been explored. We aimed to evaluate the impact of <i>F5</i> rs6025 and <i>SERPINE1</i> rs6092 on invasive mechanical ventilation (IMV) requirement and the levels of coagulation proteins among patients with severe COVID-19. Four-hundred fifty-five patients with severe COVID-19 were genotyped using TaqMan assays. Coagulation-related proteins (P-Selectin, D-dimer, P-selectin glycoprotein ligand-1, tissue plasminogen activator [tPA], plasminogen activator inhibitor-1, and Factor IX) were assessed by cytometric bead arrays in one- and two-time determinations. Accordingly, <i>SERPINE1</i> rs6092, P-Selectin (GG 385 pg/mL vs. AG+AA 632 pg/mL, <i>p</i> = 0.0037), and tPA (GG 1858 pg/mL vs. AG+AA 2546 pg/mL, <i>p</i> = 0.0284) levels were different. Patients carrying the CT <i>F5</i>-rs6025 genotype exhibited lower levels of factor IX (CC 17,136 pg/mL vs. CT 10,247 pg/mL, <i>p</i> = 0.0355). Coagulation proteins were also different among IMV patients than non-IMV. PSGL-1 levels were significantly increased in the late stage of COVID-19 (>10 days). The frequencies of <i>F5</i> rs6025 and <i>SERPINE1</i> rs6092 variants were not different among IMV and non-IMV. The <i>SERPINE1</i> rs6092 variant is related to the impaired coagulation process in patients with COVID-19 severe.https://www.mdpi.com/2079-7737/11/4/595COVID-19coagulationgenetics<i>F5</i><i>SERPINE1</i>PSGL-1 |
| spellingShingle | Ingrid Fricke-Galindo Ivette Buendia-Roldan Leslie Chavez-Galan Gloria Pérez-Rubio Rafael de Jesús Hernández-Zenteno Espiridión Ramos-Martinez Armando Zazueta-Márquez Felipe Reyes-Melendres Aimé Alarcón-Dionet Javier Guzmán-Vargas Omar Andrés Bravo-Gutiérrez Teresa Quintero-Puerta Ilse Adriana Gutiérrez-Pérez Alejandro Ortega-Martínez Enrique Ambrocio-Ortiz Karol J. Nava-Quiroz José Luis Bañuelos-Flores María Esther Jaime-Capetillo Mayra Mejía Jorge Rojas-Serrano Ramcés Falfán-Valencia <i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital Biology COVID-19 coagulation genetics <i>F5</i> <i>SERPINE1</i> PSGL-1 |
| title | <i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital |
| title_full | <i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital |
| title_fullStr | <i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital |
| title_full_unstemmed | <i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital |
| title_short | <i>SERPINE1</i> rs6092 Variant Is Related to Plasma Coagulation Proteins in Patients with Severe COVID-19 from a Tertiary Care Hospital |
| title_sort | i serpine1 i rs6092 variant is related to plasma coagulation proteins in patients with severe covid 19 from a tertiary care hospital |
| topic | COVID-19 coagulation genetics <i>F5</i> <i>SERPINE1</i> PSGL-1 |
| url | https://www.mdpi.com/2079-7737/11/4/595 |
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