Effect of Extremely Low-Frequency (50 Hz) Field on Proliferation Rate of Human Adipose-Derived Mesenchymal Stem Cells
Background: The effects of non-ionizing extremely low-frequency electromagnetic fields (ELF-EMF) chronic exposure on human beings due to its potential health hazards has become a focus of interest since many years ago. Stem cells are useful models for assessment of effects of ELF-EMF on other cell l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2013-06-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2724 |
| Summary: | Background: The effects of non-ionizing extremely low-frequency electromagnetic fields (ELF-EMF) chronic exposure on human beings due to its potential health hazards has become a focus of interest since many years ago. Stem cells are useful models for assessment of effects of ELF-EMF on other cell lines and human beings. Adipose tissue has been known source of multipotent stromal cells (MSCs), which can be obtained by a less invasive method and in large amounts compared with bone marrow stromal cells (BMSCs); so this study was done on human adipose-derived stem cells (hADSCs). The effect of ELF-EMF with intensity of 0.5 and 1 mT and 50 Hz on proliferation rates of hADSCs at 20 and 40 min/day for 7 days was assessed.
Methods: MTT assay was used to determine the growth and metabolism of cells and Trypan blue test was also done for cell viability.
Findings: The proliferation rate and growth of hADSCs in all exposure groups was significantly higher than that in sham groups except in group of 1 mT, 40 min/day (P < 0.05).
Conclusion: The results showed that 0.5 and 1 mT magnetic strength fields can promote the proliferation rates of the hMSCs derived adipose tissue regarding the duration of exposure. |
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| ISSN: | 1027-7595 1735-854X |