Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat
Background: Developing effective therapeutic strategies to delay the progression of chronic kidney disease (CKD) remains a significant challenge. Low-intensity pulsed ultrasound (LIPUS) has demonstrated potential for treating CKD, but the underlying molecular mechanisms are still elusive. This study...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402308739X |
| _version_ | 1797429853263233024 |
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| author | Zhiqiang Ouyang Guodong Zhang Weipeng Wang Lishi Shao Xiaolan Du Guocheng Li Na Tan Xinyan Zhou Jun Yang Lin Huang Chengde Liao |
| author_facet | Zhiqiang Ouyang Guodong Zhang Weipeng Wang Lishi Shao Xiaolan Du Guocheng Li Na Tan Xinyan Zhou Jun Yang Lin Huang Chengde Liao |
| author_sort | Zhiqiang Ouyang |
| collection | DOAJ |
| description | Background: Developing effective therapeutic strategies to delay the progression of chronic kidney disease (CKD) remains a significant challenge. Low-intensity pulsed ultrasound (LIPUS) has demonstrated potential for treating CKD, but the underlying molecular mechanisms are still elusive. This study aimed to evaluate the therapeutic efficacy of LIPUS and to elucidate the involved genes and signaling pathways. Methods: The CKD model was established in rats using Adriamycin (ADR). The bilateral kidneys of CKD rats were continuously stimulated with LIPUS for a period of four weeks. The therapeutic efficacy was defined by renal function and histopathological evaluation. RNA sequencing was employed to profile the transcriptome of rat kidneys in each group. Cluster analysis was utilized to identify differentially expressed genes (DEGs), followed by enrichment analysis of their associated pathways using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Results: LIPUS treatment improved ADR-induced renal dysfunction in the CKD group. Renal fibrosis and pathological damages were also alleviated in the ADR + LIPUS group compared to the ADR group. Cluster analysis identified 844 DEGs. GO enrichment analysis revealed enrichment in inflammatory response terms, while KEGG enrichment analysis highlighted the nuclear factor kappa B (NF-κB) signaling and ferroptosis-related pathways. Conclusion: Continuous LIPUS treatment improved ADR-induced renal fibrosis and dysfunction. The therapeutic effect of LIPUS was primarily due to its ability to suppress the CKD-related inflammation, which was associated with the modulation of the NF-κB and ferroptosis signaling pathways. These findings provide a new insight into the potential molecular mechanisms of LIPUS in treating CKD. Further research is necessary to confirm these findings and to identify potential therapeutic targets within these pathways. |
| first_indexed | 2024-03-09T09:18:53Z |
| format | Article |
| id | doaj.art-76101440deeb44b5be5dc849254eef5b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:18:53Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-76101440deeb44b5be5dc849254eef5b2023-12-02T07:02:40ZengElsevierHeliyon2405-84402023-11-01911e21531Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease ratZhiqiang Ouyang0Guodong Zhang1Weipeng Wang2Lishi Shao3Xiaolan Du4Guocheng Li5Na Tan6Xinyan Zhou7Jun Yang8Lin Huang9Chengde Liao10Department of Radiology, Yan′an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming 650051, ChinaDepartment of Resource Management, Yunnan Cancer Hospital (The Third Affiliated Hospital of Kunming Medical University), Kunming 650100, ChinaSchool of Electronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu 611731, ChinaDepartment of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650033, ChinaDepartment of Radiology, Yan′an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming 650051, ChinaDepartment of Radiology, Yan′an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming 650051, ChinaDepartment of Radiology, Yan′an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming 650051, ChinaDepartment of Radiology, Yan′an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming 650051, ChinaDepartment of Radiology, Yunnan Cancer Hospital (The Third Affiliated Hospital of Kunming Medical University), Kunming 650100, ChinaSchool of Electronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu 611731, China; Corresponding author.Department of Radiology, Yan′an Hospital of Kunming City (Yanan Hospital Affiliated to Kunming Medical University), Kunming 650051, China; Corresponding author.Background: Developing effective therapeutic strategies to delay the progression of chronic kidney disease (CKD) remains a significant challenge. Low-intensity pulsed ultrasound (LIPUS) has demonstrated potential for treating CKD, but the underlying molecular mechanisms are still elusive. This study aimed to evaluate the therapeutic efficacy of LIPUS and to elucidate the involved genes and signaling pathways. Methods: The CKD model was established in rats using Adriamycin (ADR). The bilateral kidneys of CKD rats were continuously stimulated with LIPUS for a period of four weeks. The therapeutic efficacy was defined by renal function and histopathological evaluation. RNA sequencing was employed to profile the transcriptome of rat kidneys in each group. Cluster analysis was utilized to identify differentially expressed genes (DEGs), followed by enrichment analysis of their associated pathways using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Results: LIPUS treatment improved ADR-induced renal dysfunction in the CKD group. Renal fibrosis and pathological damages were also alleviated in the ADR + LIPUS group compared to the ADR group. Cluster analysis identified 844 DEGs. GO enrichment analysis revealed enrichment in inflammatory response terms, while KEGG enrichment analysis highlighted the nuclear factor kappa B (NF-κB) signaling and ferroptosis-related pathways. Conclusion: Continuous LIPUS treatment improved ADR-induced renal fibrosis and dysfunction. The therapeutic effect of LIPUS was primarily due to its ability to suppress the CKD-related inflammation, which was associated with the modulation of the NF-κB and ferroptosis signaling pathways. These findings provide a new insight into the potential molecular mechanisms of LIPUS in treating CKD. Further research is necessary to confirm these findings and to identify potential therapeutic targets within these pathways.http://www.sciencedirect.com/science/article/pii/S240584402308739XChronic kidney diseaselow intensity pulsed ultrasoundTranscriptomicsNuclear factor kappa BFerroptosis |
| spellingShingle | Zhiqiang Ouyang Guodong Zhang Weipeng Wang Lishi Shao Xiaolan Du Guocheng Li Na Tan Xinyan Zhou Jun Yang Lin Huang Chengde Liao Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat Heliyon Chronic kidney disease low intensity pulsed ultrasound Transcriptomics Nuclear factor kappa B Ferroptosis |
| title | Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat |
| title_full | Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat |
| title_fullStr | Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat |
| title_full_unstemmed | Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat |
| title_short | Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat |
| title_sort | transcriptome profile analysis revealed the potential mechanism of lipus treatment for adriamycin induced chronic kidney disease rat |
| topic | Chronic kidney disease low intensity pulsed ultrasound Transcriptomics Nuclear factor kappa B Ferroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S240584402308739X |
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