Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus
Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.9.1125 |
| _version_ | 1828259800227512320 |
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| author | Rene van Wezel Huangting Liu Po Tien John Stanley Yiguo Hong |
| author_facet | Rene van Wezel Huangting Liu Po Tien John Stanley Yiguo Hong |
| author_sort | Rene van Wezel |
| collection | DOAJ |
| description | Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized. |
| first_indexed | 2024-04-13T03:15:17Z |
| format | Article |
| id | doaj.art-7610242e3fcf4c609da0e4eeb007d38d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:15:17Z |
| publishDate | 2001-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-7610242e3fcf4c609da0e4eeb007d38d2022-12-22T03:04:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-09-011491125112810.1094/MPMI.2001.14.9.1125Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the NucleusRene van WezelHuangting LiuPo TienJohn StanleyYiguo HongExpression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.9.1125BegomovirusGeminiviridaeputative transcriptional activator protein |
| spellingShingle | Rene van Wezel Huangting Liu Po Tien John Stanley Yiguo Hong Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus Molecular Plant-Microbe Interactions Begomovirus Geminiviridae putative transcriptional activator protein |
| title | Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus |
| title_full | Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus |
| title_fullStr | Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus |
| title_full_unstemmed | Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus |
| title_short | Gene C2 of the Monopartite Geminivirus Tomato yellow leaf curl virus-China Encodes a Pathogenicity Determinant That Is Localized in the Nucleus |
| title_sort | gene c2 of the monopartite geminivirus tomato yellow leaf curl virus china encodes a pathogenicity determinant that is localized in the nucleus |
| topic | Begomovirus Geminiviridae putative transcriptional activator protein |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.9.1125 |
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