Establishment and maintenance of thyroid organoids from human cancer cells

Establishment and maintenance of thyroid organoids from human cancer cells

Summary: Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification...

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Bibliographic Details
Main Authors: Valeria Pecce, Marialuisa Sponziello, Simone Bini, Giorgio Grani, Cosimo Durante, Antonella Verrienti
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cancer
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002738

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